Post-translational Modifications of bZIP Transcription Factors in Abscisic Acid Signaling and Drought Responses

Joo, Hyunhee; Baek, Woonhee; Lim, Chae Woo; Lee, Sung Chul

doi:10.2174/1389202921999201130112116

Cited by 26 publications

(16 citation statements)

References 115 publications

(156 reference statements)

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“…This was consistent with results of studies of polar [7] and Malus halliana [45]. Both gene Several studies have demonstrated the roles of plant bZIP proteins in numerous developmental processes and in responses to biotic and abiotic stresses [8,[49][50][51][52]. However, little is known about their functions in C. paliurus.…”

Section: Discussionsupporting

confidence: 88%

Genome-wide identification and analysis of bZIP gene family reveal their roles during development and drought stress in Wheel Wingnut (Cyclocarya paliurus)

et al. 2022

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Background The bZIP gene family has important roles in various biological processes, including development and stress responses. However, little information about this gene family is available for Wheel Wingnut (Cyclocarya paliurus). Results In this study, we identified 58 bZIP genes in the C. paliurus genome and analyzed phylogenetic relationships, chromosomal locations, gene structure, collinearity, and gene expression profiles. The 58 bZIP genes could be divided into 11 groups and were unevenly distributed among 16 C. paliurus chromosomes. An analysis of cis-regulatory elements indicated that bZIP promoters were associated with phytohormones and stress responses. The expression patterns of bZIP genes in leaves differed among developmental stages. In addition, several bZIP members were differentially expressed under drought stress. These expression patterns were verified by RT-qPCR. Conclusions Our results provide insights into the evolutionary history of the bZIP gene family in C. paliurus and the function of these genes during leaf development and in the response to drought stress. In addition to basic genomic information, our results provide a theoretical basis for further studies aimed at improving growth and stress resistance in C. paliurus, an important medicinal plant.

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Section: Discussionsupporting

confidence: 88%

Genome-wide identification and analysis of bZIP gene family reveal their roles during development and drought stress in Wheel Wingnut (Cyclocarya paliurus)

et al. 2022

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“…Numerous transcription factors, including HD‐ZIP and bZIP, have been found to undergo post‐translational modification during the responses of plants to hormones and environmental stimuli (Himmelbach et al ., 2002; Hill, 2015; Skelly et al ., 2016; Joo et al ., 2021). In our previous study, we demonstrated that CaDRHB1, a member of the HD‐ZIP I subfamily, functions as a positive modulator for drought tolerance via ABA‐mediated stomatal closure (Lim et al ., 2018).…”

Section: Resultsmentioning

confidence: 99%

Pepper SUMO E3 ligase CaDSIZ1 enhances drought tolerance by stabilizing the transcription factor CaDRHB1

2022

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Summary Small ubiquitin‐like modifier (SUMO) conjugation (SUMOylation) is a reversible post‐translational modification associated with protein stability and activity, and modulates hormone signaling and stress responses in plants. Previously, we reported that the pepper dehydration‐responsive homeobox domain transcription factor CaDRHB1 acts as a positive modulator of drought response. Here, we show that CaDRHB1 protein stability is enhanced by SUMO E3 ligase Capsicum annuum DRHB1‐interacting SAP and Miz domain (SIZ1) (CaDSIZ1)‐mediated SUMOylation in response to drought, thereby positively modulating abscisic acid (ABA) signaling and drought responses. Substituting lysine (K) 138 of CaDRHB1 with arginine reduced CaDSIZ1‐mediated SUMOylation, indicating that K138 is the principal site for SUMO conjugation. Virus‐induced silencing of CaDSIZ1 promoted CaDRHB1 degradation, suggesting that CaDSIZ1 is involved in drought‐induced SUMOylation of CaDRHB1. CaDSIZ1 interacted with and facilitated SUMO conjugation of CaDRHB1. CaDRHB1, mainly localized in the nucleus, but also in the cytoplasm in the SUMOylation mimic state, suggesting that SUMOylation of CaDRHB1 promotes its nuclear export, leading to cytoplasmic accumulation. Moreover, CaDSIZ1‐silenced pepper plants were less sensitive to ABA and considerably sensitive to drought stress, whereas CaDSIZ1‐overexpressing plants displayed ABA‐hypersensitive and drought‐tolerant phenotypes. Collectively, our data indicate that CaDSIZ1‐mediated SUMOylation of CaDRHB1 functions in ABA‐mediated drought tolerance.

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“…Among the differentially expressed TF-coding genes, TF families with known functions were represented; however, the exact functions of most of these genes remain unknown. The role of some members of TF families, such as ARF, bZIP, MYB-related, and WRKY, which exhibited differential regulation among potato cultivars, are well known in root responses to drought stress ( Jiang et al., 2017 ; Joo et al., 2021 ; Wei et al., 2021 ; Yang et al., 2021 ). Differential regulation of the same gene family members in different potato cultivars may result in different regulatory networks that can determine cultivar-specific rooting depth and root response to drought stress.…”

Section: Discussionmentioning

confidence: 99%

Global transcriptome and coexpression network analyses reveal cultivar-specific molecular signatures associated with different rooting depth responses to drought stress in potato

Qin

Ali²,

Wang³

et al. 2022

Front. Plant Sci.

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Potato is one of the most important vegetable crops worldwide. Its growth, development and ultimately yield is hindered by drought stress condition. Breeding and selection of deep-rooted and drought-tolerant potato varieties has become a prime approach for improving the yield and quality of potato (Solanum tuberosum L.) in arid and semiarid areas. A comprehensive understanding of root development-related genes has enabled scientists to formulate strategies to incorporate them into breeding to improve complex agronomic traits and provide opportunities for the development of stress tolerant germplasm. Root response to drought stress is an intricate process regulated through complex transcriptional regulatory network. To understand the rooting depth and molecular mechanism, regulating root response to drought stress in potato, transcriptome dynamics of roots at different stages of drought stress were analyzed in deep (C119) and shallow-rooted (C16) cultivars. Stage-specific expression was observed for a significant proportion of genes in each cultivar and it was inferred that as compared to C16 (shallow-rooted), approximately half of the genes were differentially expressed in deep-rooted cultivar (C119). In C16 and C119, 11 and 14 coexpressed gene modules, respectively, were significantly associated with physiological traits under drought stress. In a comparative analysis, some modules were different between the two cultivars and were associated with differential response to specific drought stress stage. Transcriptional regulatory networks were constructed, and key components determining rooting depth were identified. Through the results, we found that rooting depth (shallow vs deep) was largely determined by plant-type, cell wall organization or biogenesis, hemicellulose metabolic process, and polysaccharide metabolic process. In addition, candidate genes responding to drought stress were identified in deep (C119) and shallow (C16) rooted potato varieties. The results of this study will be a valuable source for further investigations on the role of candidate gene(s) that affect rooting depth and drought tolerance mechanisms in potato.

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Post-translational Modifications of bZIP Transcription Factors in Abscisic Acid Signaling and Drought Responses

Cited by 26 publications

References 115 publications

Genome-wide identification and analysis of bZIP gene family reveal their roles during development and drought stress in Wheel Wingnut (Cyclocarya paliurus)

Genome-wide identification and analysis of bZIP gene family reveal their roles during development and drought stress in Wheel Wingnut (Cyclocarya paliurus)

Pepper SUMO E3 ligase CaDSIZ1 enhances drought tolerance by stabilizing the transcription factor CaDRHB1

Global transcriptome and coexpression network analyses reveal cultivar-specific molecular signatures associated with different rooting depth responses to drought stress in potato

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