Post-translational modifications of Trypanosoma cruzi histone H4

Cunha, Júlia Pinheiro Chagas da; Nakayasu, Ernesto; Almeida, Igor C.; Schenkman, Sérgio

doi:10.1016/j.molbiopara.2006.08.012

Cited by 67 publications

(88 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells were treated with DNAse again, and the second round of supernatants were saved again as a DNAse-released fraction (DNAse released 2). Samples were resolved by SDS-PAGE and analyzed by western blot using anti-anti-hsp70 (1:2,000), 28 antihistone H4 (1:100), 29 and anti-TcPCNA antibodies as described above.…”

Section: Methodsmentioning

confidence: 99%

Trypanosoma cruziDNA replication includes the sequential recruitment of pre-replication and replication machineries close to nuclear periphery

Calderano¹,

Godoy²,

Motta³

et al. 2011

Nucleus

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Trypanosoma cruziDNA replication includes the sequential recruitment of pre-replication and replication machineries close to nuclear periphery

Calderano¹,

Godoy²,

Motta³

et al. 2011

Nucleus

Self Cite

View full text Add to dashboard Cite

“…To further test the specificity of the antibodies, a peptide was designed corresponding to the 23 N-terminal amino acids of T. cruzi histone H4 containing the identified acetylated lysines (Lys-4, Lys-10, Lys-14) (17). This peptide was recognized by ␣-acH4 antibodies and competed for H4 binding in Western blot when antibodies were preincubated with the peptide (supplemental Fig.…”

Section: Methodsmentioning

confidence: 99%

Histone Acetylation and Methylation at Sites Initiating Divergent Polycistronic Transcription in Trypanosoma cruzi

Respuela

Ferella

Rada-Iglesias

et al. 2008

Journal of Biological Chemistry

View full text Add to dashboard Cite

Trypanosomes are ancient eukaryotic parasites in which the protein-coding genes, organized in large polycistronic clusters on both strands, are transcribed from as yet unidentified promoters. In an effort to reveal transcriptional initiation sites, we examined the Trypanosoma cruzi genome for histone modification patterns shown to be linked to active genes in various organisms. Here, we show that acetylated and methylated histones were found to be enriched at strand switch regions of divergent gene arrays, not at convergent clusters or intra-and intergenic regions within clusters. The modified region showed a bimodular profile with two peaks centered over the 5-regions of the gene pair flanking the strand switch region. This pattern, which demarcates polycistronic transcription units originating from bidirectional initiation sites, is likely to be common in kinetoplastid parasites as well as in other organisms with polycistronic transcription. In contrast, no acetylation was found at promoters of the highly expressed rRNA and spliced leader genes or satellite DNA or at tested retrotransposonal elements. These results reveal, for the first time, the presence of specific epigenetic marks in T. cruzi with potential implications for transcriptional regulation; they indicate that both histone modifications and bidirectional transcription are evolutionarily conserved.

show abstract

“…They also contain variants of H2, H3, and H4, namely H2Az, H2BV, and H3V. Like most trypanosome genes that are orthologous to those defined in characterized systems, the trypanosome histones also diverge substantially from those in well-studied organisms (8,23,28,36). Small group modifications, mainly acetylation and methylation that occur on lysines and arginines, are present and reflect the importance of the histone code throughout evolution.…”

Section: Epigenetic Control Of Transcriptionmentioning

confidence: 99%

RNA Polymerase Transcription Machinery in Trypanosomes

2008

View full text Add to dashboard Cite

Transcription is a fundamental biological process employed by all living organisms to decode their genetic information. The information stored in genomic DNA is copied into RNA molecules by polymerization of ribonucleotide building blocks, which ultimately gives rise to different classes of transcripts. mRNAs encode polypeptides, rRNAs drive the macromolecular protein-synthesis machinery, and tRNAs act as adaptor molecules to assemble amino acids into proteins. Synthesis of specific transcripts is influenced by environmental and internal cell signals, which in turn are pivotal for the control of cellular regulatory networks.Trypanosomes are unicellular parasitic protozoa, members of the order Kinetoplastidae, which diverged early during evolution. They cause a wide range of debilitating diseases in humans and domestic animals. Trypanosoma brucei, known as the African trypanosome, is transmitted by tsetse flies in subSaharan Africa (15). Infection fulminates into African sleeping sickness in humans and nagana in animals (3). T. brucei is a digenetic parasite that cycles as a procyclic form in the digestive tract of the tsetse vector and as an extracellular bloodstream form in its mammalian host. During its complex life cycle, the parasite passes through five successive morphologically distinct forms (39). Parasites change from the procyclic form, which is characterized by a procyclic-specific surface coat, through two morphologically distinct forms in the fly and then they emerge as long, slender bloodstream forms, covered with a variant surface glycoprotein coat. Once inside the mammalian host, the long slender bloodstream form actively divides and establishes parasitemia. In the late phases of infection, the morphology of the parasite changes to nondividing short stumpy forms, which are ready to be taken up by the insect during a blood meal. The bloodstream form, with a rudimentary mitochondrion, is perfectly adapted to utilize the abundant supply of glucose from the mammalian blood and generate sufficient energy by glycolysis. The insect form, on the other hand, has a functional mitochondrion and generates most of its energy by respiration. These necessary metabolic adaptations depend upon a precise orchestration of numerous metabolic and cell biological activities.Studies of trypanosomes have uncovered several unusual biological phenomena (6). Notable among them are trans splicing and RNA editing (reviewed in references 7, 35, 46, 48, and 59). Protein-coding genes in trypanosomes are transcribed as long polycistronic precursor RNAs. Individual mature mRNAs are formed by trans splicing of a 39-nucleotide spliced leader (SL) RNA at the 5Ј end and subsequent 3Ј end maturation. RNA editing, used to produce mitochondrial mRNA, requires extensive alterations of primary transcripts by guide RNAs. Although guide RNA-dependent RNA editing is uniquely observed in trypanosomes, trans splicing has subsequently been observed in several other lower eukaryotes, including nematodes, trematodes, euglenoids, and chordates. The...

show abstract

Post-translational modifications of Trypanosoma cruzi histone H4

Cited by 67 publications

References 29 publications

Trypanosoma cruziDNA replication includes the sequential recruitment of pre-replication and replication machineries close to nuclear periphery

Trypanosoma cruziDNA replication includes the sequential recruitment of pre-replication and replication machineries close to nuclear periphery

Histone Acetylation and Methylation at Sites Initiating Divergent Polycistronic Transcription in Trypanosoma cruzi

RNA Polymerase Transcription Machinery in Trypanosomes

Contact Info

Product

Resources

About