Post-traumatic moderate systemic hyperthermia worsens behavioural outcome after spinal cord injury in the rat

Urdzíková, Lucia Machová; Vanický, Ivo

doi:10.1038/sj.sc.3101792

Cited by 12 publications

(6 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The animals treated with MSCs scored, on average, 3.9 points higher than untreated controls [1]. However, there was extremely high variability between the studies (improvement from 0.3 to 10 points, when compared to controls), which is connected to the different types and severities of lesion models, as well as the varying methods of MSC application [17,18,19]. In our previous studies, we applied rat MSCs directly into the lesion that had been isolated from either bone marrow [20] or adipose tissue [21].…”

Section: Resultsmentioning

confidence: 99%

Human Mesenchymal Stem Cells Modulate Inflammatory Cytokines after Spinal Cord Injury in Rat

Urdzíková

Růžička

LaBagnara

et al. 2014

IJMS

105

View full text Add to dashboard Cite

Transplantation of mesenchymal stem cells (MSC) improves functional recovery in experimental models of spinal cord injury (SCI); however, the mechanisms underlying this effect are not completely understood. We investigated the effect of intrathecal implantation of human MSC on functional recovery, astrogliosis and levels of inflammatory cytokines in rats using balloon-induced spinal cord compression lesions. Transplanted cells did not survive at the lesion site of the spinal cord; however, functional recovery was enhanced in the MSC-treated group as was confirmed by the Basso, Beattie, and Bresnahan (BBB) and the flat beam test. Morphometric analysis showed a significantly higher amount of remaining white matter in the cranial part of the lesioned spinal cords. Immunohistochemical analysis of the lesions indicated the rearrangement of the glial scar in MSC-treated animals. Real-time PCR analysis revealed an increased expression of Irf5, Mrc1, Fgf2, Gap43 and Gfap. Transplantation of MSCs into a lesioned spinal cord reduced TNFα, IL-4, IL-1β, IL-2, IL-6 and IL-12 and increased the levels of MIP-1α and RANTES when compared to saline-treated controls. Intrathecal implantation of MSCs reduces the inflammatory reaction and apoptosis, improves functional recovery and modulates glial scar formation after SCI, regardless of cell survival. Therefore, repeated applications may prolong the beneficial effects induced by MSC application.

show abstract

Section: Resultsmentioning

confidence: 99%

Human Mesenchymal Stem Cells Modulate Inflammatory Cytokines after Spinal Cord Injury in Rat

Urdzíková

Růžička

LaBagnara

et al. 2014

IJMS

105

View full text Add to dashboard Cite

show abstract

“…In models of global and focal ischemia, for example, induced periods of hyperthermia during or following the insult significantly aggravated histopathological damage and worsen behavioral outcomes (Chopp et al, 1988; Dietrich et al, 1990; Wang et al, 2009). In traumatic spinal cord injury, exacerbated structural damage and behavioral outcomes with hyperthermia has also been reported (Urdzikova and Vanicky, 2006; Yu et al, 2001). The underlying mechanisms responsible for the worsened histopathological and behavioral outcomes observed with hyperthermia remain to be delineated in these various CNS injuries (Campos et al, 2013; Dietrich and Bramlett, 2007).…”

Section: Discussionmentioning

confidence: 99%

Emergence of cognitive deficits after mild traumatic brain injury due to hyperthermia

Titus

Furones

Atkins

et al. 2015

Experimental Neurology

View full text Add to dashboard Cite

Mild elevations in core temperature can occur in individuals involved in strenuous activities that are risky for potentially sustaining a mild traumatic brain injury (mTBI) or concussion. Recently, we have discovered that mild elevations in brain temperature can significantly aggravate the histopathological consequences of mTBI. However, whether this exacerbation of brain pathology translates into behavioral deficits is unknown. Therefore, we investigated the behavioral consequences of elevating brain temperature to mildly hyperthermic levels prior to mTBI. Adult male Sprague Dawley rats underwent mild fluid-percussion brain injury or sham surgery while normothermic (37 °C) or hyperthermic (39 °C) and were allowed to recover for 7 days. Animals were then assessed for cognition using the water maze and cue and contextual fear conditioning. We found that mTBI alone at normothermia had no effect on long-term cognitive measures whereas mTBI animals that were hyperthermic for 15 min prior to and for 4 h after brain injury were significantly impaired on long-term retention for both the water maze and fear conditioning. In contrast, hyperthermic mTBI animals cooled within 15 min to normothermia demonstrated no significant long-term cognitive deficits. Mild TBI irrespective of temperature manipulations resulted in significant short-term working memory deficits. Cortical atrophy and contusions were detected in all mTBI treatment groups and contusion volume was significantly less in hyperthermic mTBI animals that were cooled as compared to hyperthermic mTBI animals that remained hyperthermic. These results indicate that brain temperature is an important variable for mTBI outcome and that mildly elevated temperatures at the time of injury result in persistent cognitive deficits. Importantly, cooling to normothermia after mTBI prevents the development of long-term cognitive deficits caused by hyperthermia. Reducing temperature to normothermic levels soon after mTBI represents a rational approach to potentially mitigate the long-term consequences of mTBI.

show abstract

“…The animals were perioperatively treated with antibiotics (ATB) prophylaxis by intramuscular injection of gentamicin sulfate (Lek Pharmaceutical, 5 mg/kg). The rat was placed onto a heating pad, which was set for 37 • C to prevent hyper/hypothermia [47]. The back of the animal was shaved and the skin colored with disinfectant.…”

Section: Spinal Cord Injury Proceduresmentioning

confidence: 99%

New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats

et al. 2020

View full text Add to dashboard Cite

Despite the variety of experimental models of spinal cord injury (SCI) currently used, the model of the ventral compression cord injury, which is commonly seen in humans, is very limited. Ventral balloon compression injury reflects the common anatomical mechanism of a human lesion and has the advantage of grading the injury severity by controlling the inflated volume of the balloon. In this study, ventral compression of the SCI was performed by the anterior epidural placement of the balloon of a 2F Fogarty’s catheter, via laminectomy, at the level of T10. The balloon was rapidly inflated with 10 or 15 μL of saline and rested in situ for 5 min. The severity of the lesion was assessed by behavioral and immunohistochemical tests. Compression with the volume of 15 μL resulted in severe motor and sensory deficits represented by the complete inability to move across a horizontal ladder, a final Basso, Beattie and Bresnahan (BBB) score of 7.4 and a decreased withdrawal time in the plantar test (11.6 s). Histology and immunohistochemistry revealed a significant loss of white and gray matter with a loss of motoneuron, and an increased size of astrogliosis. An inflation volume of 10 μL resulted in a mild transient deficit. There are no other balloon compression models of ventral spinal cord injury. This study provided and validated a novel, easily replicable model of the ventral compression SCI, introduced by an inflated balloon of Fogarty´s catheter. For a severe incomplete deficit, an inflated volume should be maintained at 15 μL.

show abstract

Post-traumatic moderate systemic hyperthermia worsens behavioural outcome after spinal cord injury in the rat

Cited by 12 publications

References 39 publications

Human Mesenchymal Stem Cells Modulate Inflammatory Cytokines after Spinal Cord Injury in Rat

Human Mesenchymal Stem Cells Modulate Inflammatory Cytokines after Spinal Cord Injury in Rat

Emergence of cognitive deficits after mild traumatic brain injury due to hyperthermia

New Model of Ventral Spinal Cord Lesion Induced by Balloon Compression in Rats

Contact Info

Product

Resources

About