Objective: To present a case of ovarian cyst rupture during transvaginal Doppler ultrasound examination. Case: A 46-year-old woman with abdominal pain referring to right groin was evaluated with abdominal ultrasonography and presented at our clinic due to a hypoechoic, nonseptated ovarian cyst with regular borders of approximately 7 cm in diameter. Following abrupt and intense pelvic pain starting with the transvaginal scan, the color Doppler revealed high velocity and irregular flow from the posterior cervicouterine wall toward the Pouch of Douglas; then, the patient's pain rapidly regressed. Conclusion: To the best of our knowledge, this is the first report revealing real-time spontaneous rupture of a "simple" ovarian cyst during Doppler sonography.