Post‐Treatment of Acid Mine Drainage Using Laboratory‐Extracted Common Mushroom Chitosan

Abstract

A post-treatment approach using common mushroom (Agaricus bisporus)derived chitosan was done to treat and purify a synthetic pretreated acid mine drainage (AMD) solution. Commercially available chitosan and laboratory-prepared chitosan were used as coagulants for post-treatment of AMD. Both chitosans were characterized by Fourier transform infrared (FTIR) and scanning electron microscopy energy-dispersive X-ray spectroscopy (SEM-EDX). The treatment underwent a batch testing with parameters of mixing speed, mixing time, and chitosan loading. The removal of heavy metals focused on copper, iron, and nickel. The optimum parameters of the commercially available chitosan were then employed for the performance of the laboratory-prepared chitosan to evaluate its capability as a post-treatment agent for AMD.

Characterization Chitosan Nanoparticles Prepared from the Stalks of Agaricus bisporus Brown and its Effect on Inhibition of Aspergillus flavus and Reducing Aflatoxin B1 Production

“…The size and shape of chitosan and chitosan nanoparticles that are dried and prepared from the stalks of A. bisporus brown are tested by scanning electron microscope (model Inspect F50) with a magnifying force (250 -120000) [17].…”

“…x Dilution factor The electronic microscope picture shows that extracted chitosan does not have heterogeneity in the surface of chitosan's molecules, as well as its roughness and containing many layers of irregularshaped flakes (chips) with cracks on some of the chitosan parts, while the shape of chitosan nanoparticles is found to be spherical. While [27], was testing the chitosan produced from A. bisporus by SEM, he found that chitosan's shape was porous when magnified 500 times, while it appeared as nano fibers when magnified 2000 times.…”