“…Convert the pollutants into less harmful products, being cost-competitive, producing less secondary pollutant and being eco-friendly are the merits of biological system over the others (Saratale et al, 2011). Many researchers evaluated the anaerobic or aerobic biodegradation of azo dye (Brás et al, 2005;Is ßik and Sponza, 2005;Buitrón et al, 2004), whereas some effort has been done on the mineralization of azo dye in sequential anaerobic-aerobic environment or integrated anaerobic-aerobic environment (Sponza and Is ßik, 2002;Ong et al, 2005;Supaka et al, 2004;Jonstrup et al, 2011;Hosseini Koupaie et al, 2011, 2013. Sequential anaerobic-aerobic system facilitated the mineralization of azo dye (Sponza and Is ßik, 2002;Hosseini Koupaie et al, 2011).…”