Post-treatment with N-acetylcysteine ameliorates endotoxin shock-induced organ damage in conscious rats

Hsu, Bang‐Gee; Lee, Ru‐Ping; Yang, Fwu-Lin; Harn, Horng‐Jyh; Chen, Hsing I.

doi:10.1016/j.lfs.2006.06.040

Cited by 93 publications

(75 citation statements)

References 34 publications

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“…The rat awakened soon after the operation and waited overnight later for intravenous TAA administration in conscious state. We have developed a simple technique to maintain the rats in a conscious state without restrain and anaesthesia as described previously (Lee et al 2002;Hsu et al 2006).…”

Section: Preparation Of Animalsmentioning

confidence: 99%

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Single dose intravenous thioacetamide administration as a model of acute liver damage in rats

Chen

Subeq

Lee

et al. 2008

Int J Experimental Path

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1Contributed equally to this study. SummaryThioacetamide (TAA) has been used extensively in the development of animal models of acute liver injury. Frequently, TAA is administered intraperitoneally to induce liver damage under anaesthesia. However, it is rarely administered by intravenous injection in conscious rats. The experiments in this study were designed to induce acute liver damage by single intravenous injection of TAA (0, 70 and 280 mg ⁄ kg) in unrestrained rats. Biochemical parameters and cytokines measured during the 60-h period following TAA administration, included white blood cells (WBC), haemoglobulin (Hb), platelet, aspartate transferase (GOT), alanine transferase (GPT), total bilirubin (TBIL), direct bilirubin (DBI), albumin, ammonia (NH3), r-glutamyl transpeptidase (r-GT), tumour necrosis factor-a (TNF-a) and interleukin-6 (IL-6). Rats were sacrificed by decapitation 60 h after TAA administration and livers were removed immediately for pathology and immunohistochemical (IHC) examination. Another group of rats were sacrificed by decapitation 1, 6 and 24 h after TAA administration and livers were removed immediately for time course change of pathology and IHC examination. TAA significantly increased blood WBC, GOT, GPT, TBIL, DBIL, NH3, r-GT, TNF-a and IL-6 levels but decreased the blood Hb, platelet and albumin level. The levels of histopathological damage in the liver after intravenous TAA administration were also increased with a dose-dependent trend and more increased at 60 h after TAA administration. The levels of inducible nitric oxide synthase (iNOS) and nuclear factor-jB (NF-jB) detected by IHC in the liver after intravenous TAA administration were also increased with a dose-dependent trend and more increased at 1 h after TAA administration. Single intravenous TAA administration without anaesthesia is a restorable animal model which may be used to investigate acute liver damage.

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Section: Preparation Of Animalsmentioning

confidence: 99%

“…Another part was stored at )80°C for later analysis of TNF-a and IL-6 concentration (Hsu et al 2006). …”

Section: Blood Sample Analysesmentioning

confidence: 99%

Single dose intravenous thioacetamide administration as a model of acute liver damage in rats

Chen

Subeq

Lee

et al. 2008

Int J Experimental Path

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“…[10] Tang et al [11] demonstrated that treatment with a GSH precursor, NAC, had prevented Group B streptococcus growth in the lung, liver and spleen in an experimental rat model induced with ethanol. In the study of Hsu et al [12] in rats, they concluded that NAC treatment suppressed the release of inflammation markers TNF-α, IL-6 and IL-10 in organ insufficiency related to endotoxin shock. It was claimed that liver, heart and kidney injuries were minimized with this useful effect of NAC.…”

Section: Discussionmentioning

confidence: 99%

The effect of N-acetyl cysteine on serum glutathione, TNF-α and tissue malondialdehyde levels in the treatment of sepsis

Gül¹,

Ayan²,

Seydanoğlu³

et al. 2011

Ulus Travma Acil Cerrahi Derg

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“…21,24,25 Tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were measured with antibody enzyme-linked immunosorbent assays (ELISA) using a commercial antibody pair, recombinant standards, and a biotin-streptavidin-peroxidase detection system (Endogen, Rockford, Illinois). All agents, samples and working standards were prepared at room temperature according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

Histopathological and biochemical changes following fat embolism with administration of corn oil micelles

Liu

Hsieh

Chen

2008

The Journal of Bone and Joint Surgery. British volume

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Several experimental models have been used to produce intravascular fat embolism. We have developed a simple technique to induce fat embolism using corn oil emulsified with distilled water to form fatty micelles. Fat embolism was produced by intravenous administration of these fatty micelles in anaesthetised rats, causing alveolar oedema, haemorrhage and increased lung weight.Histopathological examination revealed fatty droplets and fibrin thrombi in the lung, kidney and brain. The arteriolar lumen was filled with fatty deposits. Following fat embolism, hypoxia and hypercapnia occurred. The plasma phospholipase A 2 , nitrate/nitrite, methylguidanidine and proinflammatory cytokines were significantly increased. Mass spectrometry showed that the main ingredient of corn oil was oleic acid.This simple technique may be applied as a new animal model for the investigation of the mechanisms involved in the fat embolism syndrome.

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Post-treatment with N-acetylcysteine ameliorates endotoxin shock-induced organ damage in conscious rats

Cited by 93 publications

References 34 publications

Single dose intravenous thioacetamide administration as a model of acute liver damage in rats

Single dose intravenous thioacetamide administration as a model of acute liver damage in rats

The effect of N-acetyl cysteine on serum glutathione, TNF-α and tissue malondialdehyde levels in the treatment of sepsis

Histopathological and biochemical changes following fat embolism with administration of corn oil micelles

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