Post-weaning effects of milk and milk components on the intestinal mucosa in inflammation

Russ, Anna E; Barnett, Matthew P. G.; McNabb, Warren C.; Anderson, Rachel C.; Reynolds, Gordon; Roy, Nicole C.

doi:10.1016/j.mrfmmm.2009.12.006

Cited by 8 publications

(4 citation statements)

References 94 publications

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“…Although some HMOs can be chemically synthesised, an alternative source of natural lactose-derived oligosaccharides is from ruminant milk. The composition of ruminant milk varies between different species and breeds, and also throughout lactation [ 5 ]. Caprine milk contains substantially less oligosaccharides than human milk (3–20 g/L) [ 6 , 7 ] at 0.25–0.30 g/L but has 5–8 times more than bovine milk (0.03–0.06 g/L) and 10 times more than ovine milk (0.02–0.04 g/L) [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

et al. 2016

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Caprine milk contains the highest amount of oligosaccharides among domestic animals, which are structurally similar to human milk oligosaccharides (HMOs). This suggests caprine milk oligosaccharides may offer similar protective and developmental effects to that of HMOs. However, to date, studies using oligosaccharides from caprine milk have been limited. Thus, this study aimed to examine the impact of a caprine milk oligosaccharide-enriched fraction (CMOF) on barrier function of epithelial cell co-cultures of absorptive enterocytes (Caco-2 cells) and mucus-secreting goblet cells (HT29-MTX cells), that more closely simulate the cell proportions found in the small (90:10) and large intestine (75:25). Treatment of epithelial co-cultures with 0.4, 1.0, 2.0 and 4.0 mg/mL of CMOF was shown to have no effect on metabolic activity but did enhance cell epithelial barrier integrity as measured by trans-epithelial electrical resistance (TEER), in a dose-dependent manner. The CMOF at the maximum concentration tested (4.0 mg/mL) enhanced TEER, mucin gene expression and mucin protein abundance of epithelial co-cultures, all of which are essential components of intestinal barrier function.

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Section: Introductionmentioning

confidence: 99%

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

et al. 2016

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“…Previous studies showed that weaning is often accompanied by the disturbance of enteric bacteria and inflammatory response (22)(23)(24) . Prolonged enteric inflammation owing to the presence of pathogens can damage intestinal epithelia, compromise nutrient absorption and subsequently lead to malnutrition and growth retardation in early childhood (25,26) . Weaning changes Etn availability to both host intestinal epithelial cells and gut microflora.…”

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confidence: 99%

Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats

et al. 2018

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Ethanolamine (Etn) contained in milk is the base constituent of phosphatidylethanolamine and is required for the proliferation of intestinal epithelial cells and bacteria, which is important for maintenance of the gut microbiome and intestinal development. The present study investigated the effect of Etn on intestinal function and microbiome using 21-d-old Sprague–Dawley rats treated with 0, 250, 500 and 1000 μmEtn in drinking water for 2 weeks immediately after weaning. Growth performance, intestinal morphology, antioxidant capacity and mucosal immunity, as well as gut microbiota community composition, were evaluated. Metagenomic prediction and metabolic phenotype analysis based on 16S RNA sequencing were also carried out to assess changes in metabolic functions. We found that weaned rats administered 500 μmEtn enhanced mucosal antioxidant capacity, as evidenced by higher superoxide dismutase and glutathione peroxidase levels in the jejunum (P<0·05) compared with those in the control group. Predominant microbes including Bacteroidetes, Proteobacteria, Elusimicrobia and Tenericutes were altered by different levels of Etn compared with the control group. An Etn concentration of 500 µmshifted colonic microbial metabolic functions that are in favour of lipid- and sugar-related metabolism and biosynthesis. Etn also altered the metabolic phenotypes such as anaerobic microbial counts, and oxidative stress tolerance at over 250 µm. This is the first report for a role of Etn in modifying gut microbiota and intestinal functions. Our findings highlighted the important role of Etn in shaping gut microbial community and promotes intestinal functions, which may provide a better insight of breast-feeding to infant’s gut health.

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“…Anti-inflammatory effects and improvements in IBD symptoms in humans have been reported for fermented milks as well as milk components (53,54). In rodent mod- …”

Section: Discussionmentioning

confidence: 99%

Attenuation of Colitis by Lactobacillus casei BL23 Is Dependent on the Dairy Delivery Matrix

Lee

Yin

Griffey

et al. 2015

Appl Environ Microbiol

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The role of the food delivery matrix in probiotic performance in the intestine is not well understood. Because probiotics are often provided to consumers in dairy products, we investigated the contributions of milk to the health-benefiting performance of Lactobacillus casei BL23 in a dextran sulfate sodium (DSS)-induced murine model of ulcerative colitis. L. casei BL23 protected against the development of colitis when ingested in milk but not in a nutrient-free buffer simulating consumption as a nutritional supplement. Consumption of (acidified) milk alone also provided some protection against weight loss and intestinal inflammation but was not as effective as L. casei and milk in combination. In contrast, L. casei mutants deficient in DltD (lipoteichoic acid D-alanine transfer protein) or RecA (recombinase A) were unable to protect against DSS-induced colitis, even when consumed in the presence of milk. Mice fed either L. casei or milk contained reduced quantities of colonic proinflammatory cytokines, indicating that the L. casei DltD ؊ and RecA ؊ mutants as well as L. casei BL23 in nutrient-free buffer were effective at modulating immune responses. However, there was not a direct correlation between colitis and quantities of these cytokines at the time of sacrifice. Identification of the cecal microbiota by 16S rRNA gene sequencing showed that L. casei in milk enriched for Comamonadaceae and Bifidobacteriaceae; however, the consumption of neither L. casei nor milk resulted in the restoration of the microbiota to resemble that of healthy animals. These findings strongly indicate that probiotic strain efficacy can be influenced by the food/supplement delivery matrix. C ertain strains of Lactobacillus and Bifidobacterium are used extensively as probiotics intended to confer health benefits upon delivery to the digestive tract in foods, beverages, or dietary supplements. Dairy products are the most popular food matrices for probiotic strains, and numerous clinical trials examining probiotic efficacy have used yogurt or (fermented) milks as carriers of probiotics to the intestine (1-3). Comparisons of data from human studies have indicated that probiotic delivery matrices, including dairy products, can influence probiotic intestinal survival and persistence (1). Remarkably, however, only a single human study and a few animal studies have compared the impacts of different carrier matrices or food product formulations on the capacity of probiotics to influence health (1, 2). Evidence from preclinical animal studies indicates that fermented milks might augment probiotic efficacy, although this has yet to be systematically investigated.Probiotics are useful for the prevention and treatment of a variety of acute and chronic diseases (4-6). Prevention and reduction of symptoms of inflammatory bowel diseases (IBDs) are among the most extensively evaluated benefits (7). Although the pathogenesis of IBDs is unclear, these diseases are characterized by chronic intestinal inflammation and excessive accumulation of reactive ox...

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Post-weaning effects of milk and milk components on the intestinal mucosa in inflammation

Cited by 8 publications

References 94 publications

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium

Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats

Attenuation of Colitis by Lactobacillus casei BL23 Is Dependent on the Dairy Delivery Matrix

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