Postfabrication encapsulation of model protein drugs in a negatively thermosensitive hydrogel

Zhang, Ying; Zhu, Wen; Wang, Biaobing; Yu, Lin; Ding, Jiandong

doi:10.1002/jps.20310

Cited by 26 publications

(14 citation statements)

References 31 publications

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“…The rate of protein release in hydrogels mainly depends on the pore size of the hydrogel, the structure and composition of the matrix and the size of the protein, as well as protein–network interactions . When the pH 7.4 buffer solution is above the PI of SS (4.3) and BSA (4.7), there is an electrostatic interaction between BSA and SS which contributes to the faster release and higher release amount of SS.…”

Section: Resultsmentioning

confidence: 99%

Controlled release of bovine serum albumin from stimuli‐sensitive silk sericin based interpenetrating polymer network hydrogels

Wang

Lian

2013

Polymer International

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In recent years, the use of stimuli‐sensitive interpenetrating polymer network (IPN) hydrogels for the encapsulation and controlled release of protein drugs has received significant attention. The purpose of the present study was to investigate the release of bovine serum albumin (BSA), a model drug, from a series of thermosensitive silk sericin (SS) poly(N‐isopropylacrylamide) and pH‐responsive SS poly(methacrylic acid) IPN hydrogels. The effect of the chemical architecture of the IPN was investigated on the percentage loading of BSA and its subsequent release from the loaded devices. The temperature and pH of the release medium were studied for their impact on the release of BSA. The pulsatile releasing behavior of IPN hydrogels revealed that they can be made into microcapsules or thermo‐valves, which act as an on–off release control. © 2013 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Controlled release of bovine serum albumin from stimuli‐sensitive silk sericin based interpenetrating polymer network hydrogels

Wang

Lian

2013

Polymer International

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“…Postfabrication encapsulation via soaking a preformed hydrogel in a drug‐containing solution is a feasible technique for loading protein drugs 37–39. The postfabrication technique23 used in this study depends on swollen hydrogels at low temperatures. Figure 3 demonstrates the thermosensitivity of the microgels prepared using the two different methods.…”

Section: Resultsmentioning

confidence: 99%

“…In contrast to conventional drug encapsulation approaches, protein drugs were, in this study, entrapped into the microgel by soaking the swollen hydrogel in an aqueous protein solution at low temperatures. Because drug loading was performed after preparation of microgels, this approach was termed as postfabrication encapsulation approach as suggested and confirmed in our previous article23 based on similar hydrogel films. BSA was used as the model protein to investigate loading and release behaviors.…”

Section: Methodsmentioning

confidence: 96%

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Preparation of thermosensitive microgels via suspension polymerization using different temperature protocols

Zhang

Zhu

Ding

2005

J Biomedical Materials Res

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A thermosensitive and biodegradable microgel for protein drug release was synthesized from a thermosensitive macromer via inverse suspension polymerization. Preparation was made under a constant temperature or under variable temperatures. In the latter protocol, dispersion was performed at a low temperature below lower critical solution temperature of the macromer aqueous solution and polymerization was performed at a high temperature above lower critical solution temperature. According to the experiments, the constant-temperature method was not suitable for preparation of microgels when the macromer concentration was high, because early physical gelation at the preparation temperature seriously influenced formation of dispersed droplets. If the macromer concentration was low, both temperature protocols resulted in spherical hydrogel microparticles, but the properties of the resulting microgels were different to a certain degree. In both cases, the model protein bovine serum albumin was loaded into microgels by a postfabrication encapsulation technique, which takes advantage of the microgels' negative thermosensitivity. The results demonstrate that, in microgel preparation, the variable-temperature protocol is useful in suspension polymerization of negatively thermosensitive macromers at a wide rage of monomer concentrations.

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“…For unambiguous, sensitive detection of analytes, it is highly desirable and sometimes required, that the analyte extraction process be specific, i.e., only the analyte and ideally no impurities are retained by the solid-phase (Chen et al 2006;Handley 1999;Zhang et al 2005;D'Orazio 2003). Unfortunately, the microfluidic devices mentioned above do not possess this attribute.…”

Section: Introductionmentioning

confidence: 99%

An aptamer-based microfluidic device for thermally controlled affinity extraction

Nguyen

Pei

Stojanović

et al. 2008

Microfluid Nanofluid

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We present a microfluidic device for specific extraction and thermally activated release of analytes using nucleic acid aptamers. The device primarily consists of a microchamber that is packed with aptamer-functionalized microbeads as a stationary phase, and integrated with a micro heater and temperature sensor. We demonstrate the device operation by performing the extraction of a metabolic analyte, adenosine monophosphate coupled with thiazole orange (TO-AMP), with high selectivity to an RNA aptamer. Controlled release of TO-AMP from the aptamer surface is then conducted at low temperatures using on-chip thermal activation. This allows isocratic analyte elution, which eliminates the use of potentially harsh reagents, and enables efficient regeneration of the aptamer surfaces when device reusability is desired.

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Postfabrication encapsulation of model protein drugs in a negatively thermosensitive hydrogel

Cited by 26 publications

References 31 publications

Controlled release of bovine serum albumin from stimuli‐sensitive silk sericin based interpenetrating polymer network hydrogels

Controlled release of bovine serum albumin from stimuli‐sensitive silk sericin based interpenetrating polymer network hydrogels

Preparation of thermosensitive microgels via suspension polymerization using different temperature protocols

An aptamer-based microfluidic device for thermally controlled affinity extraction

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