Postharvest Highbush Blueberry Fruit Antimicrobial Volatile Profiles in Relation to Anthracnose Fruit Rot Resistance

Polashock, James J.; Saftner, Robert A.; Kramer, Matthew

doi:10.21273/jashs.132.6.859

Cited by 20 publications

(9 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The evaluation of plants for anthracnose fruit rot resistance can only be accomplished when they bear fruit in sufficient quantities for inoculation, usually at 2–3 years of age. In previous studies, anthracnose fruit rot resistance in blueberries was not correlated with resistance to foliar infection (Ehlenfeldt et al ., 2006) or the production of antimicrobial fruit volatiles (Polashock et al ., 2007). The identification of anthracnose fruit rot resistance mechanisms and associated molecular markers could improve resistance screening procedures.…”

Section: Introductionsupporting

confidence: 60%

Identification of differentially expressed genes in a resistant versus a susceptible blueberry cultivar after infection by Colletotrichum acutatum

Miles¹,

Day

Schilder

2011

Molecular Plant Pathology

View full text Add to dashboard Cite

Anthracnose fruit rot, caused by the fungus Colletotrichum acutatum, is an important disease of blueberry worldwide. The cultivar Elliott is resistant, severely restricting fungal growth and sporulation relative to the susceptible cultivar Jersey. The objectives of this research were as follows: (i) to analyse pre-penetration events in 'Elliott' and 'Jersey' at different stages of fruit development; (ii) to identify putative defence genes in 'Elliott' fruit; and (iii) to monitor the timing of the oxidative burst in both cultivars. Light microscopy revealed no differences in the pre-penetration activities of C. acutatum on the immature fruit of both cultivars. However, at fruit ripening, conidia germinated and formed appressoria more rapidly on 'Jersey' than on 'Elliott' fruit. Using suppression subtractive hybridization, 37 differentially expressed sequence tags (ESTs) were detected in 'Elliott' versus 'Jersey' upon infection. Several of the ESTs had homology to known plant defence genes, such as a class II chitinase, pathogenesis-related protein 10 (PR10) and β-1,3-glucanase. Two putative genes involved in oxidative stress were identified: a metallothionein-like protein and monodehydroascorbate reductase. ESTs of fungal origin were also detected. Many ESTs had no homology to known genes. Using semi-quantitative and quantitative reverse transcription-polymerase chain reaction (RT-PCR), the expression of most of the candidate genes was detected earlier in 'Elliott' than in 'Jersey', some within 24 h post inoculation (hpi). Monitoring of the oxidative burst showed that the overall H(2) O(2) concentration was two to three times higher in 'Elliott' than in 'Jersey' at 24 hpi. The elucidation of the basis of resistance to C. acutatum in blueberry will facilitate the development of new anthracnose fruit rot-resistant cultivars.

show abstract

Section: Introductionsupporting

confidence: 60%

Identification of differentially expressed genes in a resistant versus a susceptible blueberry cultivar after infection by Colletotrichum acutatum

Miles¹,

Day

Schilder

2011

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…In previous studies, resistance to fruit infection by C. acutatum was not correlated with resistance to foliar infection (Ehlenfeldt et al. , 2006) or with the production of antimicrobial fruit volatiles (Polashock et al. , 2007).…”

Section: Introductionmentioning

confidence: 87%

Evaluation of screening methods and fruit composition in relation to anthracnose fruit rot resistance in blueberries

et al. 2011

View full text Add to dashboard Cite

Anthracnose fruit rot is an important disease of blueberries, and losses are common in humid growing regions. Most commercial cultivars are susceptible and the disease is usually managed with fungicides. However, a few cultivars are considered resistant. The objectives of this study were to: (i) compare different inoculation techniques for anthracnose fruit rot resistance screening, (ii) screen ripe fruit from a range of blueberry cultivars using selected techniques, and (iii) investigate the role of fruit characteristics in anthracnose fruit rot resistance. The following inoculation methods were evaluated on ripe fruit of a susceptible and resistant cultivar using a conidial suspension: spray, droplet, and injection inoculation of whole fruit; and droplet inoculation of the open surface of cut fruit. All whole-fruit inoculations yielded similar results. Despite the removal of the epidermis, resistance was also expressed in cut fruit but relatively fewer conidia were produced. The cut-fruit assay required substantially less time and half the amount of fruit to accomplish than whole-fruit assays. Detached ripe fruit from 24 cultivars in 2008 and 26 cultivars in 2009 were screened for resistance. Results from the cut-fruit assay correlated best with published resistance ratings. To determine the possible role of fruit characteristics in resistance, fruit pH, titratable acidity, sugar content and firmness were regressed against various fruit rot resistance measures. Fruit rot resistance was positively correlated with sugar content. On defined media, mycelial growth was restricted as sugar concentration increased and pH decreased, suggesting that fruit composition may play a role in the resistance phenotype.

show abstract

“…This compound altered the structure of the conidial cell wall and plasma membrane, causing disorganization and lysis of organelles, and eventually, cell death (1). In blueberries, many of these compounds were also identified but the quantity of the volatile compounds was not correlated with anthracnose fruit rot resistance in the various blueberry cultivars (38,39).…”

Section: Passive Host Defensesmentioning

confidence: 99%

Host Defenses Associated with Fruit Infection by Colletotrichum Species with an Emphasis on Anthracnose of Blueberries

Miles

Schilder

2013

Plant Health Progress

View full text Add to dashboard Cite

Defense mechanisms in fruit against invasion by fungal pathogens fall into several categories, including fruit physiological factors, passive defense responses, and active defense responses. Colletotrichum spp. are common fruit rot pathogens on a variety of crops. The infection strategy of Colletotrichum pp. varies depending upon the host and the tissue type being colonized and range from hemibiotrophy to necrotrophy. Anthracnose fruit rot caused by C. acutatum is the most important postharvest disease of blueberries (Vaccinium corymbosum). In order to better understand host resistance mechanisms in blueberry fruits, the resistant cultivar Elliott was compared with the susceptible cultivar Jersey. Higher levels of hydrogen peroxide and differentially expressed defense-related genes were found in ‘Elliott’ than in ‘Jersey’ fruit after inoculation. Furthermore, higher levels of anthocyanins and flavonols, including two distinctive compounds, were observed in Elliott fruits and two unique flavonols were present in Elliott. Additionally, pH and sugar content were implicated as being involved in host resistance in blueberry fruits. The results from these studies provide a significant contribution to the understanding of the multiple factors that contribute to anthracnose fruit rot resistance in blueberry. Accepted for publication 15 August 2013. Published 25 November 2013.

show abstract

Postharvest Highbush Blueberry Fruit Antimicrobial Volatile Profiles in Relation to Anthracnose Fruit Rot Resistance

Cited by 20 publications

References 49 publications

Identification of differentially expressed genes in a resistant versus a susceptible blueberry cultivar after infection by Colletotrichum acutatum

Identification of differentially expressed genes in a resistant versus a susceptible blueberry cultivar after infection by Colletotrichum acutatum

Evaluation of screening methods and fruit composition in relation to anthracnose fruit rot resistance in blueberries

Host Defenses Associated with Fruit Infection by Colletotrichum Species with an Emphasis on Anthracnose of Blueberries

Contact Info

Product

Resources

About