Postirradiation volatile secretions of mice: Syngeneic and allogeneic immune and behavioral effects

Surinov, B. P.; Isaeva, V. G.; Dukhova, N. N.

doi:10.1007/s10517-005-0106-8

Cited by 6 publications

(5 citation statements)

References 5 publications

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“…In the past we have successfully used an approach where nonirradiated companion animals are placed in close proximity to irradiated animals. Our group have conducted several experiments with irradiated fish [25e27] sharing aquarium water with unirradiated fish, and Surinov's group in Russia [28,29] and our group [7] have also demonstrated communication between irradiated mice and their cage mates with subsequent signal expression in the nonirradiated animals. These experiments parallel in vitro "medium transfer" experiments in that the unirradiated animals receiving signals from irradiated animals were never anywhere near the radiation source and never had any part of their bodies exposed to Xrays.…”

Section: Introductionmentioning

confidence: 99%

Use of synchrotron medical microbeam irradiation to investigate radiation-induced bystander and abscopal effects in vivo

et al. 2015

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The question of whether bystander and abscopal effects are the same is unclear. Our experimental system enables us to address this question by allowing irradiated organisms to partner with unexposed individuals. Organs from both animals and appropriate sham and scatter dose controls are tested for expression of several endpoints such as calcium flux, role of 5HT, reporter assay cell death and proteomic profile. The results show that membrane related functions of calcium and 5HT are critical for true bystander effect expression. Our original inter-animal experiments used fish species whole body irradiated with low doses of X-rays, which prevented us from addressing the abscopal effect question. Data which are much more relevant in radiotherapy are now available for rats which received high dose local irradiation to the implanted right brain glioma. The data were generated using quasi-parallel microbeams at the biomedical beamline at the European Synchrotron Radiation Facility in Grenoble France. This means we can directly compare abscopal and "true" bystander effects in a rodent tumour model. Analysis of right brain hemisphere, left brain and urinary bladder in the directly irradiated animals and their unirradiated partners strongly suggests that bystander effects (in partner animals) are not the same as abscopal effects (in the irradiated animal). Furthermore, the presence of a tumour in the right brain alters the magnitude of both abscopal and bystander effects in the tissues from the directly irradiated animal and in the unirradiated partners which did not contain tumours, meaning the type of signal was different.

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Section: Introductionmentioning

confidence: 99%

Use of synchrotron medical microbeam irradiation to investigate radiation-induced bystander and abscopal effects in vivo

et al. 2015

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“…During the last decade, evidence has been accumulating that bystander signals can be transmitted from irradiated animals to non-irradiated animals (Surinov et al, 2004, Mothersill et al, 2007, Isaeva and Surinov, 2007, Isaeva and Surinov, 2011, Audette-Stuart, (2011) Woenckhaus (1930. It occurred to us that this model might be useful to exclude the possibility that the effects we saw were due to systemic factors because as the rat was never exposed to radiation and merely shared a cage with the irradiated rat, any effects had to be due to transmitted signals and not intra-animal signalling.…”

Section: Introductionmentioning

confidence: 99%

Transmission of Signals from Rats Receiving High Doses of Microbeam Radiation to Cage Mates: An Inter-Mammal Bystander Effect

et al. 2013

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Inter-animal signaling from irradiated to non-irradiated organisms has been demonstrated for whole body irradiated mice and also for fish. The aim of the current study was to look at radiotherapy style limited exposure to part of the body using doses relevant in preclinical therapy. High dose homogenous field irradiation and the use of irradiation in the microbeam radiation therapy mode at the European Synchrotron Radiation Facility (ESRF) at Grenoble was tested by giving high doses to the right brain hemisphere of the rat. The right and left cerebral hemispheres and the urinary bladder were later removed to determine whether abscopal effects could be produced in the animals and also whether effects occurred in cage mates housed with them. The results show strong bystander signal production in the contra-lateral brain hemisphere and weaker effects in the distant bladder of the irradiated rats. Signal strength was similar or greater in each tissue in the cage mates housed for 48hrs with the irradiated rats. Our results support the hypothesis that proximity to an irradiated animal induces signalling changes in an unirradiated partner. If similar signaling occurs between humans, the results could have implications for caregivers and hospital staff treating radiotherapy patients.

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“…Recently the transmission of signals between animals has been documented. Both mice (Surinov et al 2004) and fish Mothersill et al 2007;Mothersill et al 2009) are proven models where one group can be irradiated and placed in proximity to a naïve group, the latter group will display the same or similar biological end points as the former. Examples of end points include: increases in sister chromatid exchange, changes in both protein and gene expression leading to mutations, cell death, micronucleus formation, neoplastic transformation (Hamada et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Injection of Resperpine into Zebrafish, Prevents Fish to Fish Communication of Radiation-Induced Bystander Signals: Confirmation in vivo of a Role for Serotonin in the Mechanism

Saroya¹,

Smith²,

Seymour³

et al. 2009

Dose-Response

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ᮀ Serotonin (5-HT) has been implicated as a potential modulator of the bystander effect in cell cultures. To assess the relevance of serotonin in vivo experiments were done with the zebrafish (Danio rerio). This species, when irradiated, transmits bystander signals to non-irradiated fish. The animals were injected with reserpine, an inhibitor of serotonin at a dose of 80mg/kg of body mass. The results show that reserpine treated fish had only 27% of the serotonin in non-treated fish. Skin tissue samples were collected from the fish and assayed for bystander signal production using a reporter bioassay. Reserpine prevented the production and communication of signals between fish. Intracellular calcium flux, identified as a bystander response in the reporter cells confirmed this. Medium harvested from tissues of X-rayed fish and their bystanders, showed an oscillating pattern of calcium flux. Samples from X-rayed fish pretreated with reserpine produced a chaotic pattern of random fluctuations in the reporter cells, while their bystander fish led to increased calcium, but no oscillations. These results suggest that 5-HT is involved in bystander signalling in zebrafish, and by decreasing the amount of available 5-HT the bystander effect can be blocked.

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Postirradiation volatile secretions of mice: Syngeneic and allogeneic immune and behavioral effects

Cited by 6 publications

References 5 publications

Use of synchrotron medical microbeam irradiation to investigate radiation-induced bystander and abscopal effects in vivo

Use of synchrotron medical microbeam irradiation to investigate radiation-induced bystander and abscopal effects in vivo

Transmission of Signals from Rats Receiving High Doses of Microbeam Radiation to Cage Mates: An Inter-Mammal Bystander Effect

Injection of Resperpine into Zebrafish, Prevents Fish to Fish Communication of Radiation-Induced Bystander Signals: Confirmation in vivo of a Role for Serotonin in the Mechanism

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