Postlabeling analysis of carcinogen-DNA adducts in mussel, Mytilus galloprovincialis

Kurelec, Branko; Chacko, Mary; Gupta, Ramesh C.

doi:10.1016/0141-1136(88)90328-5

Cited by 39 publications

(10 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, the consistent pathological changes observed in the present study in the lysosomal system of Mytilus edulis collected from urban-associated sites in Puget Sound could be attributed to the high tissue concentrations of contaminants, particularly organic contaminants, although some toxic elements such as copper and zinc could be involved as well. The above pathological changes in the digestive cells of mussels could result from: (1) a direct interaction of contaminants with lysosomal membranes, (2) biotransformation of organic contaminants into more cytotoxic products and (3) oxyradicals generated within a n d outside the lysosomal membrane a s a result of redoxcycling of organic contaminants (Anderson 1985, Stegeman 1985, Nott & Moore 1987, Varanasi et al 1987, Kurelec et al 1988, Livingstone et al 1989, Winston et al 1991. Although tissue burdens of PAHs, PCBs, and selected toxic metals in mussels were significantly correlated with impaired lysosomal function, detoxification enzyme activity (Krishnakumar unpubl.…”

mentioning

confidence: 99%

Effect of environmental contaminants on the health of Mytilus edulis from Puget Sound, Washington, USA. I. Cytochemical measures of lysosomal responses in the digestive cells using automatic image analysis

Krishnakumar¹,

Casillas²,

Varanasi³

1994

Mar. Ecol. Prog. Ser.

132

View full text Add to dashboard Cite

Lysosomal responses in the digestive cells of Mytilus eduljs from 9 sites in Puget Sound, Washington. USA, with varying degrees of sediment contamination were measured using automatic image analysis. Mussels from urban-associated sltes (areas with elevated sediment concentration of anthropogenic contaminants such as PAHs, PCBs. DDTs, and toxic elements) showed pathological changes in the lysosomes (reduced lysosomal labilization period, reduced lysosomal stability and increased N-acetyl P-hexosaminidase activity), enhanced lipofuscin deposition and increased accumulat~on of lysosomal and cytoplasmic unsaturated neutral lipids. Morphometric measures of growth showed that mussels were generally smaller in size and that somatic tissue weight relative to shell length was lower in mussels from urban-associated sites relative to those from minimally contaminated sites. Highly significant correlations were observed between concentrations of tissue PAH, PCB, and selected toxic elements (measures of anthropogenic exposure) and lysosomal labil~zation period. Pathological alterations in the lysosomal system of mussels from the urban-associated sites were probably induced by the high tissue concentrations of organic and inorganic contaminants rather than as a result of potential confounding biotic (spawning stress) or abiotic (temperature, salinity and food availability) factors. The results show that cytochemical measurement of lysosomal responses combined with automatic image analysis have the potential to be used as sensitive, accurate and rapid techniques for assessing the biological impact of environmental contaminants in mussels in the near coastal environment.

show abstract

mentioning

confidence: 99%

Effect of environmental contaminants on the health of Mytilus edulis from Puget Sound, Washington, USA. I. Cytochemical measures of lysosomal responses in the digestive cells using automatic image analysis

Krishnakumar¹,

Casillas²,

Varanasi³

1994

Mar. Ecol. Prog. Ser.

132

View full text Add to dashboard Cite

show abstract

“…It has been suggested that the extent of binding of carcinogenic xenobiotics, such as metabolites of PAHs, to DNA in organisms may serve as a dosimeter at the targetsite level. Determination of PAH-DNA adducts has been successfully applied by means of 32p postlabeling in environmentally exposed humans (15,16), fish (17)(18)(19)(20), earthworms (21), mussels (22), and plants (23). Moreover, wild woodchucks living in PAH-contaminated areas exhibited higher benzo[a]pyrene-protein adducts in blood compared to animals living in control areas (24 Other heavy metals significantly contribute to air pollution as well.…”

mentioning

confidence: 99%

Possible Relevance of Pigeons as an Indicator Species for Monitoring Air Pollution

Schilderman¹,

Hoogewerff²,

Schooten³

et al. 1997

Environmental Health Perspectives

View full text Add to dashboard Cite

“…This view is also expressed by Ray et al (1992) and is reflected in the findings of Kurelec et al (1989) who reported similarities in adduct profiles (when comparing polluted versus relatively clean sites) in the Livers of whales and various fish respectively. There was no evidence of 'endogenous' adducts as reported by Kurelec for some (but not all) aquatic species (Kurelec et al 1988(Kurelec et al , 1989. This difference may be due to minor differences in the assay protocol which will affect both sensitivity and the nature of the adducts detected.…”

Section: Discussionmentioning

confidence: 68%

Genetic toxicity in dab Limanda limanda from the North Sea

Chipman¹,

Marsh²,

Livingstone³

et al. 1992

Mar. Ecol. Prog. Ser.

View full text Add to dashboard Cite

During the Bremerhaven Workshop, a battery of assays was employed aimed at the detection of genetic damage in dab Limanda limanda and at an understanding of the influence of pollution on the metabolism of chemical pro-mutagens. The presence of chernicaVDNA adducts was investigated by 32P-postlabelling (for bulky hydrophobic adducts) and by HPLC with electrochemical detection (for oxidised deoxyguanosine). No significant differences were seen between the various sites analysed along the transect. Bacterial mutagenicity assays (Salmonella typhimurium strains TA100 and TA98) were also unable to detect frame-shift or base-pair mutagens in liver extracts or in bile samples following treatment with the deconjugating enzyme, P-glucuronidase. There was no evidence of pollution-related unscheduled DNA synthesis in dab hepatocytes on the day after sampling. Unscheduled DNA synthesis was, however, induced by nitroquinoline oxide (but not 2-acetylaminofluorene) in dab hepatocytes from 2 stations of different pollution status. The extent of excision repair was similar at the 2 stations. Regarding the metabolism of pro-mutagens, liver enzymes were capable of converting 2-acetylaminofluorene to bacterial mutagens; a process requiring N-hydroxylation and deacetylation.

show abstract

Postlabeling analysis of carcinogen-DNA adducts in mussel, Mytilus galloprovincialis

Cited by 39 publications

References 5 publications

Effect of environmental contaminants on the health of Mytilus edulis from Puget Sound, Washington, USA. I. Cytochemical measures of lysosomal responses in the digestive cells using automatic image analysis

Effect of environmental contaminants on the health of Mytilus edulis from Puget Sound, Washington, USA. I. Cytochemical measures of lysosomal responses in the digestive cells using automatic image analysis

Possible Relevance of Pigeons as an Indicator Species for Monitoring Air Pollution

Genetic toxicity in dab Limanda limanda from the North Sea

Contact Info

Product

Resources

About