Postmortem biochemistry performed on vitreous humor after postmortem CT-angiography

Abstract

a b s t r a c tPostmortem angiography is becoming increasingly essential in forensic pathology as an adjunct to con-ventional autopsy. Despite the numerous advantages of this technique, some questions have been raised regarding the influence of the contrast agent injected on the results of toxicological and biochemical anal-yses. The aim of this study was to investigate the effect of the injection of the contrast agent Angiofil Ò , mixed with paraffin oil, on the results of postmortem biochemical investigations performed on vitreous humor. Postmortem biochemical investigations were performed on vitreous samples collected from bodies that had undergone postmortem angiography (n = 50) and from a control group (n = 50). Two vit-reous samples were analyzed for each group and the results compared. Glucose, urea, creatinine, 3-b-hydroxybutyrate, sodium and chloride were tested. Different values were observed between the first and second samples in each group. However, these differences were not clinically relevant, suggesting that the injection of this contrast agent mixture does not modify the concentration of the analyzed sub-stances in the vitreous humor.

Vitreous humour as an alternative material for the determination of alcohol concentration in human corpses

“…W ostatnich latach wysoką przydatność VH w pośmiertnych badaniach biochemicznych potwierdzili Graphher i wsp., którzy po zastosowaniu angiografii metodą tomografii komputerowej (angio-CT) wykazali brak wpływu środków kontrastujących na rozkład czy zmianę ilościową substancji chemicznych, takich jak: sód, chlor, glukoza, kreatynina, mocznik [21]. Z perspektywy diagnostycznej fakt ten wydaje się bardzo obiecujący.…”

Postmortem computed tomography angiography, contrast medium administration and toxicological analyses in urine

“…Nevertheless, postmortem interval length may markedly influence the concentration of numerous molecules in many biological fluids collected after death. We therefore recommended sampling vitreous for postmortem biochemistry as soon as possible following corpse arrival at the morgue in order to prevent changes in analyte concentration from occurring [3]. The results of the study presented herein seem to indicate that differences may be observed between urine samples collected prior to and post angiography in the number of identified molecules in relation to specific cases.…”

“…It has been demonstrated that the injection of a specific contrast agent mixture consisting of Angiofil™ (a mixture of esters, mainly ethyl esters, of polyiodinated fatty acids) mixed with paraffin oil (paraffinum liquidum) failed to influence vitreous humor composition and hence specific molecule measurements, such as vitreous glucose and beta-hydroxybutyrate [3].…”

Valores de magnésio no humor vítreo de bovinos abatidos para consumo no sul do Estado Do Rio De Janeiro

“…Aproximadamente 1,0 ml do líquido foi transferido para um frasco estéril e centrifugado a 3000g por 15 minutos (Grabherr et al, 2012). O sobrenadante foi aspirado com pipeta descartável, acondicionado em frascos sem conservantes e mantido a -20 °C até o momento das análises.…”