Postmortem blood concentrations of organophosphorus pesticides

Determination of pesticides in postmortem blood and bone marrow of pesticide treated rabbits

“…Therefore, SPE was preferred for both endosulfan and diazinon analyses in the present study. The recovery ratios obtained were 93-100% for diazinon and 85-100% for endosulfan, in agreement with the literature [14,17]. Recovery ratios of certain organophosphorus pesticides such as parathion or malathion were reported to be lower compared to paraquat, whereas recovery of organochlorine compounds was higher than 72% in samples from putrefactive materials [7,21].…”

“…In previous studies dealing with fatal organophosphorus poisoning cases, investigation of blood samples revealed a range of diazinon levels of 0.4-2.77 mg/l in Poklis et al and 0.24-2.82 mg/l in Park et al [14,23]. In our study, the concentrations of diazinon in blood samples ranged between 0.1 and 0.8 mg/l, with a mean of 0.4 mg/l.…”

“…On the other hand, it has been stated that SPE was more reliable for lipophilic organophosphorus compounds and organochlorines. The recovery ratios were reported to be 70% and 87.92% in previous studies investigating diazinon and certain organophosphorus pesticides in human blood using SPE [14,17]. Therefore, SPE was preferred for both endosulfan and diazinon analyses in the present study.…”

“…A great number of methodologies exist for sample preparation prior to analysis of pesticides from biological and environmental samples. In many cases, the methods employ complex extraction protocols that can be time and solvent consuming and prone to experimental errors [14,15]. In our study, methods that were implemented in the literature were overviewed and modified in order to create more efficient and quicker methods for tissue extraction of both diazinon and endosulfan.…”

Optimization and Validation of a novel Nebulizer-assisted Liquid Phase Microextraction Followed by HPLC-DAD for Diazinon Analysis in Plasma Samples

“…Accordingly, determining diazinon in biological fluids in acute poisoning cases is the major issue in clinical and forensic toxicology [7,9]. Several analytical methods have been demonstrated for diazinon analysis in biosamples for clinical and forensic toxicological settings [10][11][12][13]. For example, Park et al developed a Solid-Phase Extraction (SPE) and Gas Chromatography/Mass Spectrometry (GC/MS) for analyzing diazinon, chlorpyrifos, malathion, and parathion in postmortem blood samples [10].…”