bass and biogenic amine-forming bacteria in canned anchovies [10][11][12]. Recently, we determined that the Sherlock MIS could confirm and update the taxonomic classification of bacteria from our culture collection in a comparison study with strains from the American Type Culture Collection [13].The purpose of this study was to rapidly identify bacteria associated with the fresh salmon microflora found on iced fish skin, gills and belly cavity by using the Sherlock MIS and to follow the spoilage microflora developing during fish storage.
Materials and Methods
Fish processingFresh seine-caught pink salmon (Oncorhynchusgorbuscha) in the Gulf of Alaska and held in chilled seawater for <24 h were procured during summer from a seafood processor in Kodiak, AK. Fish without physical damage were iced and brought to the Kodiak Seafood and Marine Science Center's pilot plant, deiced, washed and maintained under iced condition till further processing. Fish were brought on two separate occasions and used for experiments to imitate commercial conditions of processing and handling of fillets.
Iced skin-on fillet storageWithin one hour, the first batch of fish (n = 3) were headed, gutted, trimmed to remove the fins and washed briefly in tap water. Dressed fish were hand-filleted into boneless, skin-on fillets and rinsed with chilled tap water to remove bloodstains and slime. These fillets were placed individually in plastic bags closed with rubber bands to prevent contact with melting ice water. The bags were placed in a tote having a loose fitting lid on layer of flaked ice, then covered with more ice and kept in a walkin cold room (4 ± 1 °C). The melting ice was replenished every 48 h for 14 days. The fish fillets had an average temperature
Abbreviations