Postnatal Administration of <i>Lactobacillus rhamnosus</i> HN001 Ameliorates Perinatal Broad‐Spectrum Antibiotic‐Induced Reduction in Myelopoiesis and T Cell Activation in Mouse Pups

Fuglsang, Eva; Krych, Łukasz; Lundsager, Mia Thorup; Nielsen, Dennis Sandris; Frøkiær, Hanne

doi:10.1002/mnfr.201800510

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…Exposure to oral antibiotics (ABX) can alter GM diversity and this can differ depending on the particular ABX treatment [6]. In mouse models, reduced GM diversity during bacterial or viral challenge is known to exacerbate diseases e verify [2,3,[6][7][8][13][14][15]. Modulation of GM following pre-treatment of adult mice with ABX resulted in exacerbated disease following subsequent IAV challenge, characterized by delayed virus clearance, reduced titers of virus-specific antibodies and impaired inflamma some-dependent responses by innate and adaptive immune cells [6,7].…”

Section: Introductionmentioning

confidence: 99%

Influenza A Virus Infection Alters Gut Microbiota Composition in Juvenile Mice

Fuglsang¹

2018

JIDTM

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The gut microbiota (GM) stimulates the immune system to facilitate appropriate immune responses at local and distal sites of virus infection. However, the impact of distal virus infections in modulating the composition of GM is less clear.Here, a mouse model was used to examine the effect of influenza a virus (IAV) infection on the composition of GM in mice that did or did not receive oral treatment with broad spectrum antibiotics (ABX). We focused on ABX treatment of 3 weeks old juvenile mice, as this represents a dynamic time in the establishment of GM and immunity, followed by mockor IAV-infection 2 weeks later. Mice were subsequently assessed for changes in body weight, viral load in the respiratory tract and the composition of GM. As anticipated, oral ABX treatment reduced the diversity of GM and ABX-treated mice showed exacerbated disease, characterized by enhanced weight loss and increased virus titers in the upper and lower airways. Surprisingly, IAV infection also increased the diversity of GM in ABX-treated mice, resulting in a microbial composition qualitatively and quantitatively different to mock-infected ABX-treated mice and IAV-infected mice that did not receive ABX treatment. Specifically, IAV infection increased the relative abundance of eight different bacteria in GM in ABX mice compared to the GM in mock-infected ABX mice. These findings confirm an important role for GM in functional immune defense against IAV infection in juvenile mice, consistent with previous studies in adult animals. Moreover, we report for the first time that distal IAV infection of the airways can enhance the diversity of an ABX-compromised GM.

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Section: Introductionmentioning

confidence: 99%

Influenza A Virus Infection Alters Gut Microbiota Composition in Juvenile Mice

Fuglsang¹

2018

JIDTM

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“…These results had good agreement with the findings of Fuglsang et al in which perinatal broad-spectrum antibiotics treatment decreased the proportions of bone marrow granulocyte-macrophage progenitor cells, mature granulocytes, spleen granulocytes and B cells in newborn mice. Furthermore, these damages induced by perinatal antibiotics administration in offspring immunity could not be restored with decreased splenic T helper cells and cytotoxic T cells in the later life[32]. On other hand, Nyangahu et al reported that oral administration of vancomycin during pregnancy and nursing not only altered the maternal immunity, as demonstrated by significantly higher levels of both total IgG and IgM in breastmilk, but also altered the immunity of pups, as demonstrated with the significant increased lymphocyte numbers and the elevated numbers of both CD4 + T cells and B cells, most notable Follicular B cells[19].…”

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confidence: 94%

“…However, the altered fecal microbial communities in our study were entirely different from other studies. Fuglsang et al reported that Cyanobacteria became the dominate phylum, but not Tenericutes, in the fecal microbiota of pregnant mice treated with a mix of ampicillin trihydrate, gentamicin sulfate and metronidazole for 7 days before delivery[32]. Nyangahu et al reported that Firmicutes became the most dominate phylum in the maternal fecal microbiota treated with vancomycin 5 days prior…”

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Impacts of ceftriaxone exposure during pregnancy on maternal gut and placental microbiota and its influence on maternal and offspring immunity in mice

et al. 2021

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This study aimed to investigate the association between microbiota found in the maternal gut and placenta, and whether ceftriaxone exposure during pregnancy could alter these microbiota, and consequently affect the immunity of the mothers and their offspring. The microbiota in the feces and placenta of the dams were comprehensively analyzed using16S rRNA sequencing. Furthermore, viable bacteria in the placentas and blood of pups were also isolated by plate cultivation then taxonomically identified in detail by clone sequencing. Serum cytokines collected from dams and pups were quantitatively profiled using Luminex. The spleen organ index of dams was significantly lower and the offspring serum interleukin-6 levels were significantly higher in ceftriaxone-treated mice compared with the control group. The maternal fecal microbiota community was drastically altered in ceftriaxone-treated mice with significantly decreased diversity, depletion of Bacteroidetes and the blooming of Tenericutes. However, the placenta microbiota was dominated by Proteobacteria especially characteristically by Ralstonia, which was distinct from the maternal gut microbiota, regardless of whether ceftriaxone treatment or not. Viable bacteria have been found in placenta and blood cultures. These results indicated that ceftriaxone exposure in pregnancy could dramatically alter maternal intestinal microbiota, which affected the immunity of the mothers and their offspring at least partly, characteristically by enhanced pro-inflammatory responses. This study also indicated that the placenta might harbor its own microbes and the microbes were distinct from maternal gut microbiota, which may not be affected by oral administration of ceftriaxone during pregnancy.

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Probiotic Lactobacillus rhamnosus species: considerations for female reproduction and offspring health

Tas,

Sati

2024

J Assist Reprod Genet

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Postnatal Administration of Lactobacillus rhamnosus HN001 Ameliorates Perinatal Broad‐Spectrum Antibiotic‐Induced Reduction in Myelopoiesis and T Cell Activation in Mouse Pups

Abstract: Postnatal administration of a single bacterial strain efficiently restores granulopoiesis and most T cell activation in neonatal mice that suffer from a perinatal antibiotic-induced compromised gut microbiota at birth.

Cited by 5 publications

References 48 publications

Influenza A Virus Infection Alters Gut Microbiota Composition in Juvenile Mice

Influenza A Virus Infection Alters Gut Microbiota Composition in Juvenile Mice

Impacts of ceftriaxone exposure during pregnancy on maternal gut and placental microbiota and its influence on maternal and offspring immunity in mice

Probiotic Lactobacillus rhamnosus species: considerations for female reproduction and offspring health

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