Postnatal differentiation and regional histological variations in the ductus epididymidis of the Congjiang Xiang pig

Meng, Lijie; Wang, Weiyong; Xu, Yiqing; Gong, Ting; Yang, Yi

doi:10.1016/j.tice.2020.101411

Cited by 5 publications

(7 citation statements)

References 36 publications

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“…The minipig is considered a convenient animal model for preclinical studies because they rapidly achieve sexual maturity (before the age of six months) and the duration of the spermatogenic cycle is short (i.e., 35 day) [36]. Although there are structural variations at the genome level between relatively large litter sizes (average litter size: 13 piglets) and small litter sizes (average litter size: 8 piglets) in Congjiang Xiang pigs for long-term grazing and inbreeding [37,38], the breed is clearly characterized by early sexual maturity [39,40]. According to our study, Congjiang Xiang pigs exhibit the onset of puberty at approximately 2 months [40], similar to Gottingen pigs, and earlier than Yucatan minipigs (3.6-4 months) and Yorkshire (around 6 months) [41].…”

Section: Discussionmentioning

confidence: 99%

“…Although there are structural variations at the genome level between relatively large litter sizes (average litter size: 13 piglets) and small litter sizes (average litter size: 8 piglets) in Congjiang Xiang pigs for long-term grazing and inbreeding [37,38], the breed is clearly characterized by early sexual maturity [39,40]. According to our study, Congjiang Xiang pigs exhibit the onset of puberty at approximately 2 months [40], similar to Gottingen pigs, and earlier than Yucatan minipigs (3.6-4 months) and Yorkshire (around 6 months) [41]. The testes of Yorkshire pigs are remarkably active in steroidogenesis with a peak at 14-28 days (about 5-10 nmol/L) after birth, remain at low activity from 60 to 150 day (2-5 nmol/L) and increase steroidogenic activity thereafter (6 months: 10-20 nmol/L) [42].…”

Section: Discussionmentioning

confidence: 99%

“…The PCR amplification was performed in a total volume of 10 µL containing 5 µL of 2 × Es Taq Master Mix, 2 µL of RNase-free dH 2 O, 1 µL of cDNA, and 1 µL each of forward and reverse primers (10 pmol/µL). Each PCR cycle included a denaturation step at 95 • C for 3 min, followed by 40 Figure S2 and Table S2) and expressed as 2 −∆∆CT [20] using samples on day 30 for calibration. The standard curve for each primer pair was run in one plate for q-PCR.…”

Section: Quantitative Real-time Pcr (Q-pcr)mentioning

confidence: 99%

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Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs

Gong

Wang

et al. 2021

Animals

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Testicular expression of taste receptor type 1 subunit 3 (T1R3), a sweet/umami taste receptor, has been implicated in spermatogenesis and steroidogenesis in mice. We explored the role of testicular T1R3 in porcine postnatal development using the Congjiang Xiang pig, a rare Chinese miniature pig breed. Based on testicular weights, morphology, and testosterone levels, four key developmental stages were identified in the pig at postnatal days 15–180 (prepuberty: 30 day; early puberty: 60 day; late puberty: 90 day; sexual maturity: 120 day). During development, testicular T1R3 exhibited stage-dependent and cell-specific expression patterns. In particular, T1R3 levels increased significantly from prepuberty to puberty (p < 0.05), and expression remained high until sexual maturity (p < 0.05), similar to results for phospholipase Cβ2 (PLCβ2). The strong expressions of T1R3/PLCβ2 were observed at the cytoplasm of elongating/elongated spermatids and Leydig cells. In the eight-stage cycle of the seminiferous epithelium in pigs, T1R3/PLCβ2 levels were higher in the spermatogenic epithelium at stages II–VI than at the other stages, and the strong expressions were detected in elongating/elongated spermatids and residual bodies. The message RNA (mRNA) levels of taste receptor type 1 subunit 1 (T1R1) in the testis showed a similar trend to levels of T1R3. These data indicate a possible role of T1R3 in the regulation of spermatid differentiation and Leydig cell function.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Quantitative Real-time Pcr (Q-pcr)mentioning

confidence: 99%

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Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs

Gong

Wang

et al. 2021

Animals

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“…In recent years, studies with Congjiang Xiang pigs have focused on the processes of their testis development, fertility, and germplasm resources (Tang et al, 2018;Meng et al, 2020;Gong et al, 2021). Carcass traits have always been an economic concern in pig breeding.…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphisms in SIRT5 gene and their association with carcass traits in Congjiang Xiang pigs

Zhang,

Wang,

Zhao

et al. 2024

Revista Brasileira De Zootecnia

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This study was conducted to investigate the polymorphism of the silence information regulator 5 (SIRT5) gene in 103 Congjiang Xiang pigs from Southwest China. We searched for SNP (single nucleotide polymorphism) loci of SIRT5 gene through sequence alignment and PCR. We obtained nine SNP loci: g.14135 A>C (intron 6), g.14247 C>A (intron 6), g.14305 C>T (exon 7), g.14335 C>T (exon 7), g.16603 T>C (intron 7), g.16613 T>C (intron 7), g.16800 G>A (intron 7), g.16812 C>G (intron 7), and g.16916 A>G (exon 8). Further analysis of SNP genotypes associated with the carcass traits of skin thickness, backfat thickness, and eye muscle area was carried out in pigs. We found that the genotypes g.14305 C>T (CC) and g.16812 C>G (CG) had certain advantages for improving the carcass traits of Congjiang Xiang pigs. The haplotype combination of the SIRT5 gene that improved skin thickness was H2H3:CCGGCTCA. These results may provide empirical support for molecular-based breeding of carcass traits in Congjiang Xiang pigs.

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“…The Congjiang Xiang pig is known for its early sexual maturity, mini size. It also has the physiology and other traits which was similarity to humans (Meng et al, 2020). Although they display promising potential as an experimental model and top-grade pork source, there are limited studies on the reproductive profile of the boars, particularly on sperm quality.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Congjiang Xiang Pig’s Sperm Quality by Flow Cytometry

Huang,

Gong,

Meng

et al. 2024

IJAR

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Background: Sperm quality plays an important role in the animal industry. The study of the sperm quality of Congjiang Xiang pigs is limited and the Rh123/PI double staining has not been used to detect the sperm quality of boars. Thus, the study aimed to evaluate the sperm quality of the Congjiang Xiang pigs and the feasibility of the flow cytometry (FCM) method in assessing sperm quality in pigs. Methods: Semen samples were evaluated for routine indicators of semen quality and using FCM with single staining and double staining to analyze the integrity of membrane and acrosome, the mitochondrial function. The sperm quality of the Congjiang Xiang pigs was compared with the Large White pigs. Result: The semen of the Congjiang Xiang pigs had a lower sperm concentration than that of the Large White pigs (P less than 0.01). There were no significant differences in sperm vitality, sperm motility and abnormality between the two breeds (P greater than 0.05). Single sperm staining revealed a lower sperm acrosomal integrity in the semen of the Congjiang Xiang pigs than that of the Large White pigs (P less than 0.01). No significant difference between the two breeds in membrane integrity and mitochondrial function was observed (P greater than 0.05). Rh123/PI double staining demonstrated that the rate of live sperm with a normal mitochondrial function of the Congjiang Xiang pigs was significantly higher than that of the Large White pigs (P less than 0.05). The rate of necrotic sperms in the semen of the Congjiang Xiang pigs was significantly lower than that in the semen of the Large White pigs (P less than 0.05). Correlation analysis revealed a positive association between the rates of live sperm in the semen of the congjiang xiang pigs and their sperm motility (P=0.051). Our results suggest that Rh123/PI double staining with flow cytometry can provide more reliable information to assess the boar sperm quality.

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Postnatal differentiation and regional histological variations in the ductus epididymidis of the Congjiang Xiang pig

Cited by 5 publications

References 36 publications

Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs

Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs

Genetic polymorphisms in SIRT5 gene and their association with carcass traits in Congjiang Xiang pigs

Evaluation of the Congjiang Xiang Pig’s Sperm Quality by Flow Cytometry

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