Purpose To evaluate results of 23-gauge transconjunctival sutureless vitrectomy in various indications of vitreoretinal diseases. Design Prospective, non-comparative study. Materials and methods Fifty-seven consecutive eyes of 57 patients underwent vitreoretinal surgery by one surgeon using the 23-gauge, two-step instrumentation developed by Dutch Ophthalmic Research Center Inc., for epiretinal membranes (n ¼ 24), macular holes (n ¼ 10), diabetic retinopathy (n ¼ 4), rhegmatogenous retinal detachment (n ¼ 3), silicone oil removal (n ¼ 10), and other procedures (n ¼ 4). Mean operative time and intraoperative and postoperative complications were recorded. At 1 month, postoperative visual acuity, anatomical results, and induced astigmatism measured by Orbscan (BAUSCH and LOMB laboratories Inc., Rochester, NY, USA) were analysed. Results Mean operative time was 32.6±21.5 min. At 1 month, mean visual acuity improved from 1.09±0.82 to 0.80±0.79 logMar. Transient postoperative hypotony was the most common complication (21.1%), resolving without treatment. There was no choroidal effusion; and no postoperative endophthalmitis was observed. There were no significant changes in mean keratometry (P ¼ 0.957), mean axis (P ¼ 0.818), and power of astigmatism (P ¼ 0.860) at 1 month. Conclusions A 23-gauge vitrectomy seems to be safe and effective for a wide range of indications for vitreoretinal surgery. Further studies are needed to compare it with 25-gauge and 20-gauge vitrectomy in larger series.