Postoperative Wound-Freshening and Delayed Skin Grafting for Deep Wounds

Rkein, Ali M.; Rorex, Jennifer N.; Litak, Jason; McGowan, Joseph P.; Donnelly, Heidi B.

doi:10.1097/dss.0000000000002089

Cited by 2 publications

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two-step skin grafting in a patient requires a long time in the hospital and has high costs. Moreover, the lack of natural skin accessory structures, such as hair follicles, sebaceous glands, and sensory nerve receptors, is also a problem for clinicians and patients [ 11 , 34 – 36 ]. A lack of early and appropriate vascularization causes graft necrosis or loosening of implanted skin substitutes, which ultimately leads to poor skin implantation [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Single-stage transplantation combined with epidermal stem cells promotes the survival of tissue-engineered skin by inducing early angiogenesis

Wang

Yang

et al. 2023

Stem Cell Res Ther

View full text Add to dashboard Cite

Background The composite transplantation of a split-thickness skin graft (STSG) combined with an acellular dermal matrix (ADM) is a promising repair method for full-thickness skin defects. Due to delayed vascularization of the ADM, no currently available engineered skin tissue is able to permanently cover full-thickness skin defects via a single-stage procedure. Epidermal stem cells (EpSCs) have been found to promote angiogenesis in the wound bed. Whether EpSCs can induce early angiogenesis of dermal substitutes and promote the survival of single-stage tissue-engineered skin transplantation needs to be further studied. Methods In vitro, rat vascular endothelial cells (RVECs) were treated with the supernatant of EpSCs cultured in ADM and stimulated for 48 h. RVECs were analysed by RNA sequencing and tube formation assays. For the in vivo experiment, 75 rats were randomly divided into five groups: ADM, ADM + EpSCs (AE), STSG, ADM + STSG (AS), and ADM + STSG + EpSCs (ASE) groups. The quality of wound healing was estimated by general observation and H&E and Masson staining. The blood perfusion volume was evaluated using the LDPI system, and the expression of vascular markers was determined by immunohistochemistry (IHC). Results The active substances secreted by EpSCs cultured in ADM promoted angiogenesis, as shown by tube formation experiments and RNA-seq. EpSCs promoted epithelialization of the ADM and vascularization of the ADM implant. The ASE group showed significantly increased skin graft survival, reduced skin contraction, and an improved cosmetic appearance compared with the AS group and the STSG control group. Conclusions In summary, our findings suggest that EpSCs promote the formation of new blood vessels in dermal substitutes and support one-step transplantation of tissue-engineered skin, and thereby provide new ideas for clinical application.

show abstract