Postpneumonectomy lung growth: a model of reinitiation of tropoelastin and type I collagen production in a normal pattern in adult rat lung.

Koh, Daniel; Roby, Jill D.; Starcher, Barry; Senior, Robert M.; Pierce, RA

doi:10.1165/ajrcmb.15.5.8918368

Cited by 35 publications

(25 citation statements)

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“…This is in accord with in situ hybridisation data, which demonstrated tropoelastin and procollagen-I mRNA expression to be uniformly distributed throughout the gas exchange area of rat lungs 3, 7 and 14 days after PNX [37]. Studies in young and adult dogs undergoing right lung PNX revealed that growth of alveolar septa is characterised by an initial disproportionate increase in interstitial cells and matrix by nearly 3.6-fold, whereas epithelial and endothelial cells increase ,2-fold compared with control lungs [14,38].…”

Section: Discussionsupporting

confidence: 90%

Neoalveolarisation contributes to compensatory lung growth following pneumonectomy in mice

Fehrenbach¹,

Voswinckel²,

Michl³

et al. 2007

Eur Respir J

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Regeneration of the gas exchange area by induction of neoalveolarisation would greatly improve therapeutic options in destructive pulmonary diseases. Unilateral pneumonectomy is an established model to remove defined portions of gas exchange area and study mechanisms of compensatory lung growth. The question of whether new alveoli are added to the residual lung after pneumonectomy in mice was addressed.Left-sided pneumonectomy was performed in 11 adult C57BL/6 mice. Alveolar numbers were analysed in lungs fixed at days 6 and 20 after pneumonectomy and in 10 age-matched controls using design-based stereology based on a physical fractionator. Post-fixation lung volume was determined by fluid displacement.Complete restoration of lung volume was observed 20 days after pneumonectomy. Alveolar numbers were significantly increased by 33% in residual right lungs at day 20 in comparison with control right lungs. In control left lungs, an average of 471¡162610 3 alveoli was estimated, 49% of which were regenerated by residual lungs at day 20. Of the newly formed alveoli seen at day 20, 74% were already present at day 6. The present data demonstrate that, in addition to growth in size of existing alveoli, neoalveolarisation contributes to restoration of the gas exchange area in adult mice and is induced early after pneumonectomy.

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Section: Discussionsupporting

confidence: 90%

Neoalveolarisation contributes to compensatory lung growth following pneumonectomy in mice

Fehrenbach¹,

Voswinckel²,

Michl³

et al. 2007

Eur Respir J

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“…ELN, which provides important structural properties to the lung, is upregulated by mechanical ventilation in premature neonatal lambs (59), baboons (56,59), and mice (11). ELN is normally expressed in the lung during development and is quiescent in healthy adult lung (52), but aberrant ELN expression is induced in fibrotic disorders of the lung (72), in pulmonary hypertension (78), and during lung growth in response to pneumonectomy (47). GPC3, which has not been previously associated with VILI, was repressed in our samples and is repressed by TGF-␤ (66).…”

Section: Discussionmentioning

confidence: 99%

Highly conserved transcriptional responses to mechanical ventilation of the lung

Kompass¹,

Deslée

Moore

et al. 2010

Physiological Genomics

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Cross-species analysis of microarray data has shown improved discriminating power between healthy and diseased states. Computational approaches have proven effective in deciphering the complexity of human disease by identifying upstream regulatory elements and the transcription factors that interact with them. Here we used both methods to identify highly conserved transcriptional responses during mechanical ventilation, an important therapeutic treatment that has injurious side effects. We generated control and ventilated whole lung samples from the premature baboon model of bronchopulmonary dysplasia (BPD), processed them for microarray, and combined them with existing whole lung oligonucleotide microarray data from 85 additional control samples from mouse, rat, and human and 19 additional ventilated samples from mouse and rat. Of the 2,531 orthologs shared by all 114 samples, 60 were modulated by mechanical ventilation [false discovery rate (FDR)-adjusted q value ( qFDR) = 0.005, ANOVA]. These included transcripts encoding the transcription factors ATF3 and FOS. Because of compelling known roles for these transcription factors, we used computational methods to predict their targets in the premature baboon model of BPD, which included elastin (ELN), gastrin-releasing polypeptide (GRP), and connective tissue growth factor (CTGF). This approach identified highly conserved transcriptional responses to mechanical ventilation and may facilitate identification of therapeutic targets to reduce the side effects of this valuable treatment.

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“…Because elastin and collagen have been shown to play an integral part in the development of alveoli [19, 20, 21]as well as in the growth of the residual lung after lobectomy [22], we examined the lungs for evidence of resynthesis of these connective tissue elements in the presence or absence of ATRA treatment. As previously reported in hamsters [23], elastase administration to mice was followed by elastin mRNA expression in the alveolar walls, as seen by in situ hybridization (fig.…”

Section: Discussionmentioning

confidence: 99%

Retinoic Acid Does Not Affect Alveolar Septation in Adult FVB Mice with Elastase-Induced Emphysema

Lucey¹,

Goldstein²,

Breuer³

et al. 2003

Respiration

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Background: Administration of all-trans retinoic acid (ATRA) to adult Sprague-Dawley rats with emphysema induced by porcine pancreatic elastase (PPE) reversed the emphysema perhaps by inducing new alveolar formation. Objective: A study was conducted to determine whether ATRA can induce new alveolar septa and reverse the airspace enlargement caused in adult mice by PPE treatment. Methods: 48 FVB mice were divided into 6 groups. Three groups received 15 µg of PPE in 0.1 ml of 0.9% saline and 3 groups received 0.1 ml of saline, intratracheally. Starting at day 22, the mice received 12 daily intraperitoneal injections of cottonseed oil, with or without ATRA (12.5 µg or 50 µg). The mice were killed for study 1 day after the last injection. Results: Measurements of plasma and lung tissue ATRA levels showed statistically significant elevated levels after the 50-µg but not after the 12.5-µg doses of ATRA. In situ hybridization studies of elastin and α₁(I) collagen mRNA expression in pulmonary parenchyma as well as in airways and blood vessels showed no effect of ATRA. Airspace size was determined by the mean linear intercept (Lm) method. The Lm of the groups receiving PPE and ATRA (46.2 ± 4.1 µm, mean ± SD) was not significantly different from the group receiving PPE and oil (47.8 ± 6.0 µm). The Lm for groups receiving saline and ATRA (40.6 ± 2.5 µm) were not significantly different from the group receiving saline and oil (41.0 ± 2.7 µm). Comparison of the fixed lung volume data and calculated internal surface area also showed no differences between the control and ATRA-treated groups. Conclusion: ATRA treatment does not affect airspace size or expression of elastin or α₁(I) collagen mRNA in adult FVB mice with PPE-induced emphysema.

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Postpneumonectomy lung growth: a model of reinitiation of tropoelastin and type I collagen production in a normal pattern in adult rat lung.

Cited by 35 publications

References 0 publications

Neoalveolarisation contributes to compensatory lung growth following pneumonectomy in mice

Neoalveolarisation contributes to compensatory lung growth following pneumonectomy in mice

Highly conserved transcriptional responses to mechanical ventilation of the lung

Retinoic Acid Does Not Affect Alveolar Septation in Adult FVB Mice with Elastase-Induced Emphysema

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