Postprandial Plasma Phospholipids in Men Are Influenced by the Source of Dietary Fat

Meikle, Peter J.; Barlow, Christopher K.; Mellett, Natalie A.; Mundra, Piyushkumar A.; Bonham, Maxine P.; Larsen, Amy E.; Cameron‐Smith, David; Sinclair, Andrew J.; Nestel, Paul J.; Wong, Gerard

doi:10.3945/jn.115.210104

Cited by 55 publications

(49 citation statements)

References 43 publications

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“…Several structurally different ceramides are among the lipid metabolites profiled by current metabolomics platforms. Recent evidence from two small short-term intervention studies found that ceramide concentration could be transiently decreased by adopting a healthy dietary pattern 15 and changes in primary dietary sources of fat 16 . The MedDiet might also exert its effect through decreasing ceramide concentration.…”

Section: Introductionmentioning

confidence: 99%

Plasma Ceramides, Mediterranean Diet, and Incident Cardiovascular Disease in the PREDIMED Trial (Prevención con Dieta Mediterránea)

et al. 2017

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Background Although in vitro studies and investigations in animal models and small clinical populations have suggested that ceramides may represent an intermediate link between over-nutrition and certain pathological mechanisms underlying cardiovascular disease (CVD), no prospective studies have investigated the association between plasma ceramides and risk of CVD. Methods The study population consisted of 980 participants from the PREDIMED trial, including 230 incident cases of CVD and 787 randomly selected participants at baseline (including 37 overlapping cases), followed for up to 7.4 years. Participants were randomized to a Mediterranean diet (MedDiet) supplemented with extra-virgin olive oil, a MedDiet supplemented with nuts, or a control diet. Plasma ceramide concentrations were measured on a liquid chromatography tandem mass spectrometry metabolomics platform. The primary outcome was a composite of non-fatal acute myocardial infarction, non-fatal stroke, or cardiovascular death. Hazard Ratios (HRs) were estimated with weighted Cox regression models, using Barlow weights to account for the case-cohort design. Results The multivariable HRs [95% confidence interval (CI)] comparing the extreme quartiles of plasma concentrations of C16:0, C22:0, C24:0 and C24:1 ceramides were 2.39 (1.49–3.83, P trend <0.001), 1.91 (1.21–3.01, P trend =0.003), 1.97 (1.21–3.01, P trend =0.004), and 1.73 (1.09–2.74, P trend =0.011), respectively. The ceramide score, calculated as a weighted sum of concentrations of four ceramides, was associated with a 2.18-fold higher risk of CVD across extreme quartiles (HR =2.18, 95% CI, 1.36–3.49, P trend <0.001). The association between baseline ceramide score and incident CVD varied significantly by treatment groups (P interaction =0.010). Participants with a higher ceramide score and assigned to either of the two active intervention arms of the trial showed similar CVD risk to those with a lower ceramide score, whereas participants with a higher ceramide score and assigned to the control arm presented significantly higher CVD risk. Changes in ceramide concentration were not significantly different between MedDiet and control groups during the first year of follow-up. Conclusions Our study documented a novel positive association between baseline plasma ceramide concentrations and incident CVD. In addition, a Mediterranean dietary intervention may mitigate potential deleterious effects of elevated plasma ceramide concentrations on CVD. Clinical Trial Registration Controlled-Trials.com number, ISRCTN35739639. http://www.isrctn.com/ISRCTN35739639

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Section: Introductionmentioning

confidence: 99%

Plasma Ceramides, Mediterranean Diet, and Incident Cardiovascular Disease in the PREDIMED Trial (Prevención con Dieta Mediterránea)

et al. 2017

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“…GTT was assessed using a Mouse Insulin ELISA kit (ALPCO Diagnostics, Salem, New Hampshire). Lipids were extracted from ~10 μL of plasma obtained from cardiac puncture using 20 volumes of chloroform:methanol (2:1) in a single‐phase extraction process, recovering all lipids in a single phase suitable for liquid chromatography–mass spectrometry analysis, as previously described …”

Section: Methodsmentioning

confidence: 99%

Scriptaid enhances skeletal muscle insulin action and cardiac function in obese mice

Gaur

Connor

Venardos

et al. 2017

Diabetes Obesity Metabolism

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Aim:To determine the effect of Scriptaid, a compound that can replicate aspects of the exercise adaptive response through disruption of the class IIa histone deacetylase (HDAC) corepressor complex, on muscle insulin action in obesity. Materials and methods:Diet-induced obese mice were administered Scriptaid (1 mg/kg) via daily intraperitoneal injection for 4 weeks. Whole-body and skeletal muscle metabolic phenotyping of mice was performed, in addition to echocardiography, to assess cardiac morphology and function.Results: Scriptaid treatment had no effect on body weight or composition, but did increase energy expenditure, supported by increased lipid oxidation, while food intake was also increased. Scriptaid enhanced the expression of oxidative genes and proteins, increased fatty acid oxidation and reduced triglycerides and diacylglycerides in skeletal muscle. Furthermore, ex vivo insulin-stimulated glucose uptake by skeletal muscle was enhanced. Surprisingly, heart weight was reduced in Scriptaid-treated mice and was associated with enhanced expression of genes involved in oxidative metabolism in the heart. Scriptaid also improved indices of both diastolic and systolic cardiac function. Conclusion:These data show that pharmacological targeting of the class IIa HDAC corepressor complex with Scriptaid could be used to enhance muscle insulin action and cardiac function in obesity. K E Y W O R D Sanimal pharmacology

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“…Of interest, although PE and PC plasmalogens have been shown to be negatively associated with many diseases, such as coronary artery disease and diabetes (Meikle et al, 2011(Meikle et al, , 2013Sutter et al, 2016), our results indicate that some plasmalogen species (PE(P-18-1/22:4)(a), PE(P-16:0/22:4), and PC(P-18:1/22:6); Figure 5) are positively associated with pathological cardiac remodeling. Finally, when considering the potential of any lipids as biomarkers, it is important to note that some lipids can be altered by different diets, and this should be considered in future studies (Meikle et al, 2015).…”

Section: Opposing Changes In Individual Lipids In Physiological Versumentioning

confidence: 99%

Lipidomic Profiles of the Heart and Circulation in Response to Exercise versus Cardiac Pathology: A Resource of Potential Biomarkers and Drug Targets

et al. 2018

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Exercise-induced heart growth provides protection against cardiovascular disease, whereas disease-induced heart growth leads to heart failure. These distinct forms of growth are associated with different molecular profiles (e.g., mRNAs, non-coding RNAs, and proteins), and targeting differentially regulated genes has therapeutic potential for heart failure. The effects of exercise on the cardiac and circulating lipidomes in comparison to disease are unclear. Lipidomic profiling was performed on hearts and plasma of mice subjected to swim endurance training or a cardiac disease model (moderate or severe pressure overload). Several sphingolipid species and phospholipids containing omega-3/6 fatty acids were distinctly altered in heart and/or plasma with exercise versus pressure overload. A subset of lipids was validated in an independent mouse model with heart failure and atrial fibrillation. This study highlights the adaptations that occur to lipid profiles in response to endurance training versus pathology and provides a resource to investigate potential therapeutic targets and biomarkers.

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Postprandial Plasma Phospholipids in Men Are Influenced by the Source of Dietary Fat

Cited by 55 publications

References 43 publications

Plasma Ceramides, Mediterranean Diet, and Incident Cardiovascular Disease in the PREDIMED Trial (Prevención con Dieta Mediterránea)

Plasma Ceramides, Mediterranean Diet, and Incident Cardiovascular Disease in the PREDIMED Trial (Prevención con Dieta Mediterránea)

Scriptaid enhances skeletal muscle insulin action and cardiac function in obese mice

Lipidomic Profiles of the Heart and Circulation in Response to Exercise versus Cardiac Pathology: A Resource of Potential Biomarkers and Drug Targets

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