Postsynaptic actions of neurotensin on preoptic-anterior hypothalamic neurons in vitro

Baldino, Frank; Wolfson, B.

doi:10.1016/0006-8993(85)90312-9

Cited by 33 publications

(12 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The response to NT observed in the present study was similar to the response observed from neurons in the hypothalamus (Baldino and Wolfson, 1985) and in the bed nucleus of the stria terminalis (Sawada et al, 1980). These responses are, (n turn, different from the effect of neurotensin on the guinea pig ileum (Williams et al, 1979) in that we never observed neurons that responded by inhibition followed by excitation.…”

Section: Discussionsupporting

confidence: 77%

“…Injection of NT into the brain or the ventricular system produces hypothermia (Martin et al, 1980;Chandra et al, 198 1;Kalivas et al, 1982a, b), changes in blood pressure (Rioux et al,198 l), and analgesia (Clineschmidt and McGuffin, 1977;Kalivas et al, 1982b;Behbehani and Pert, 1984). Iontophoretic or pressure injection of NT can increase (Young et al, 1978;Miletic and Randic, 1979;Marwaha et al, 1980;Stanzione and Zieglgansberger, 1983;Behbehani and Pert, 1984;Baldino and Wolfson, 1985) or decrease (Guyenet and Aghajanian, 1977) firing rates of neurons in several regions of the brain. Immunohistochemical and receptor binding studies have shown that NT is heterogeneously distributed throughout the CNS (Uhl and Snyder, 1976;Kobayashi et al, 1977;Lazarus et al, 1977;Sanders et al, 1980;Young and Kuhar, 1981;Jennes et al, 1982;Quirion et al, 1982).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of neurotensin on neurons in the periaqueductal gray: an in vitro study

Behbehani¹,

Shipley²,

McLean³

1987

J. Neurosci.

View full text Add to dashboard Cite

The effect of neurotensin (NT) on periaqueductal gray (PAG) neurons was measured with extra- and intracellular recording methods in an in vitro preparation. Neurons excited by NT were heterogeneously distributed, being preferentially located in a region of PAG rich in NT-like fiber immunoreactivity. The majority of the responsive neurons were located in regions surrounding the aqueduct and the medial part of PAG. In 90% of the neurons, application of NT produced excitation that lasted for more than 2 min, while in the remaining cells, the excitatory effect lasted for less than 1 min. An inhibitory action of NT was rarely observed. Intracellular recordings showed that NT produced a depolarization leading to an increase in the spontaneous activity and multiple spiking with only a slight decrease in membrane resistance. The excitatory effect of NT was observed in neurons that were maintained in a solution containing cobalt. These results support the hypothesis that NT action on PAG neurons is due to the depolarization of the membrane and that this effect is mediated through a postsynaptic mechanism.

show abstract

Section: Discussionsupporting

confidence: 77%

mentioning

confidence: 99%

Effect of neurotensin on neurons in the periaqueductal gray: an in vitro study

Behbehani¹,

Shipley²,

McLean³

1987

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…This may be supported by an in vitro electrophysiologic experiment which has demonstrated that perfu sion of NT-containing medium produced an excitation in most of preoptic neurons in cultured preparations [2], This explanation, however, seems to be discordant with the ap parent absence of an effect of NT microinjection on LH secretion in estrogen-unprimed animals.…”

Section: Discussionmentioning

confidence: 98%

“…A putative neurotransmitter role of this peptide has been suggested on the basis of its presence in hypothalamic neurons [7,26], its release from hypothalamic tissue [15], an alteration of neuronal activity [2] and a modulation of various hypotha lamic functions such as body temperature [17] and anterior pituitary regulation [14,25], As for the control of gonadotropin secretion, Vijayan and McCann [24] reported an inhibition of luteinizing hor mone (LH) secretion in unanesthetized ovariectomized rats after injection of the peptide into the 3rd ventricle. Stimu lation of the hormone release has also been reported after NT injection into the preoptic area of anesthetized ovariec tomized rats [5], suggesting that this brain area, which com prises neurons containing immunoreactive LH-releasing hormone (LHRH) [19], may be at least one of the sites of NT action.…”

mentioning

confidence: 99%

Effects of Preoptic Microinjection of Neurotensin on Luteinizing Hormone Secretion in Unanesthetized Ovariectomized Rats with or without Estrogen Priming

Akema¹,

Praputpittaya²,

Kimura³

1987

Neuroendocrinology

View full text Add to dashboard Cite

Effects of microinjection of neurotensin (NT) into the medial preoptic area on luteinizing hormone (LH) release were examined in unanesthetized ovariectomized rats with or without estrogen priming. Microinjection of NT (100 ng) into the medial preoptic area of ovariectomized estrogen-primed rats significantly facilitated the circadian afternoon rise of LH secretion as compared to the hormone values in control animals microinjected with physiological saline. The timing of the afternoon LH rise was not altered by the peptide. In ovariectomized estrogen-unprimed rats, on the other hand, NT did not affect the existing pulsatile LH secretion nor induced an afternoon rise of the hormone secretion. These results show that NT can stimulate the preoptic LH release mechanism which undergoes the circadian clock under the influence of estrogen.

show abstract

“…In addition to its well‐documented effects on dopamine (DA) transmission (Nemeroff and Cain, 1985; Deutch and Zahm, 1992; Fuxe et al, 1992; Rostène et al, 1992; Sotty et al, 1998), the tridecapeptide neurotensin (NT; Carraway and Leeman, 1973) has been shown to increase in vivo glutamate release in discrete rat brain regions, such as the striatum, globus pallidus, frontal cortex, and substantia nigra (Sanz et al, 1993; Ferraro et al, 1995, 1998, 2001). Furthermore, previous in vitro data indicated that the peptide significantly enhanced glutamate efflux in cortical slices (Ferraro et al, 2000), glutamate‐mediated parabrachial nucleus‐ventrobasal thalamus transmission (Saleh, 1997), and the excitatory effects of microiontophoretically applied glutamate on preoptic‐anterior hypothalamic neurons (Baldino and Wolfson, 1985). Taken together, these findings suggest that NT may play a relevant role in reinforcing the effects exerted by glutamate on a variety of central nervous system functions.…”

mentioning

confidence: 94%

Neurotensin enhances glutamate excitotoxicity in mesencephalic neurons in primary culture

Antonelli

Tomasini

Finetti

et al. 2002

J of Neuroscience Research

View full text Add to dashboard Cite

The tridecapeptide neurotensin has been demonstrated to increase glutamate release in discrete rat brain regions, leading to the hypothesis of a possible involvement of the peptide in neurodegenerative pathologies. The role of neurotensin in modulating glutamate excitotoxicity and the possible neuroprotective action of the neurotensin receptor antagonist SR48692 were investigated in primary cultures of mesencephalic neurons by measuring [(3)H]dopamine uptake and tyrosine hydroxylase immunocytochemistry 24 hr after glutamate treatment. The exposure to glutamate (30 and 100 microM, 10 min) decreased [(3)H]dopamine uptake into mesencephalic neurons. Neurotensin (10 and 100 nM), added before glutamate (30 microM) exposure, significantly enhanced the glutamate-induced reduction of [(3)H]dopamine uptake. In addition, the peptide (10 nM) also significantly enhanced the effect of 100 microM glutamate. The effects of neurotensin were counteracted by the neurotensin receptor antagonist SR48692 (100 nM) and by the protein kinase C inhibitor calphostin C. The exposure to 100 microM, but not 30 microM, glutamate significantly reduced the number of tyrosine hydroxylase-immunoreactive cells, and neurotensin (10 nM) significantly enhanced this effect. SR48692 (100 nM) prevented the neurotensin-induced action. These findings support the view of a possible pathophysiological role of neurotensin in mesencephalic dopamine neuronal function. Furthermore, selective neurotensin antagonists in combination with conventional drug treatments could provide a novel therapeutic approach for the treatment of neurodegenerative disorders, such as Parkinson's disease.

show abstract

Postsynaptic actions of neurotensin on preoptic-anterior hypothalamic neurons in vitro

Cited by 33 publications

References 36 publications

Effect of neurotensin on neurons in the periaqueductal gray: an in vitro study

Effect of neurotensin on neurons in the periaqueductal gray: an in vitro study

Effects of Preoptic Microinjection of Neurotensin on Luteinizing Hormone Secretion in Unanesthetized Ovariectomized Rats with or without Estrogen Priming

Neurotensin enhances glutamate excitotoxicity in mesencephalic neurons in primary culture

Contact Info

Product

Resources

About