2007
DOI: 10.1371/journal.pcbi.0030057
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Posttranscriptional Expression Regulation: What Determines Translation Rates?

Abstract: Recent analyses indicate that differences in protein concentrations are only 20%–40% attributable to variable mRNA levels, underlining the importance of posttranscriptional regulation. Generally, protein concentrations depend on the translation rate (which is proportional to the translational activity, TA) and the degradation rate. By integrating 12 publicly available large-scale datasets and additional database information of the yeast Saccharomyces cerevisiae, we systematically analyzed five factors contribu… Show more

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Cited by 169 publications
(174 citation statements)
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“…Whilst quantitative PCR generally supported the luminescence data, the magnitude of expression was far greater at the transcript level, suggesting that there may be a translational bottleneck that inhibits full realization of potential toxin production from an increased stx gene transcript copy number (Díaz et al, 2011;Niebauer et al, 2004). The abundance and availability of tRNAs, amino acids and ribosomes have been suggested as limiting factors in protein production (Brockmann et al, 2007). Moreover, convergence of the induced single and double lysogen luminescence levels observed in the presence of a recovery step further supports the idea of a translational ceiling, as the double lysogen luminescence is unable to increase appreciably over that of the unrecovered culture, whereas single lysogen expression rises to approach double lysogen levels.…”
Section: Discussionmentioning
confidence: 88%
“…Whilst quantitative PCR generally supported the luminescence data, the magnitude of expression was far greater at the transcript level, suggesting that there may be a translational bottleneck that inhibits full realization of potential toxin production from an increased stx gene transcript copy number (Díaz et al, 2011;Niebauer et al, 2004). The abundance and availability of tRNAs, amino acids and ribosomes have been suggested as limiting factors in protein production (Brockmann et al, 2007). Moreover, convergence of the induced single and double lysogen luminescence levels observed in the presence of a recovery step further supports the idea of a translational ceiling, as the double lysogen luminescence is unable to increase appreciably over that of the unrecovered culture, whereas single lysogen expression rises to approach double lysogen levels.…”
Section: Discussionmentioning
confidence: 88%
“…Generally, mRNA concentrations are broadly employed as a surrogate for protein expression. However, various studies evaluating mRNA and protein expression on a global scale point toward their partial correspondence (53). Approximately, it has been assessed that only 20 -40% of protein expression is determined by their analogous mRNA concentrations (54,55).…”
Section: Discussionmentioning
confidence: 99%
“…As proteins are the actual cellular players for most genes, one would expect to have a positive correlation between expression levels of genes and concentrations of proteins they make. However, it is not uncommon to see only a partial correlation between gene expression and the amount of functional protein in a given region (Brockmann et al 2007). This is because of the following: First, numerous posttranscriptional regulation mechanisms such as mRNA processing, export, localization, and translation could affect the overall translational efficiency (Gingold and Pilpel 2011;Mata et al 2005).…”
Section: Discussionmentioning
confidence: 99%