Posttranscriptional gene silencing in nuclei

Hoffer, Paul; Ivashuta, Sergey; Pontes, Olga; Vitins, Alexa P.; Pikaard, Craig S.; Mroczka, Andrew; Wagner, Nicholas; Voelker, Toni A.

doi:10.1073/pnas.1009805108

Cited by 83 publications

(73 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result is contrary to the earlier belief that RNA silencing is a cytoplasmic event and intron does not trigger RNA degradation, which has been shown, for example, by using viral vector in plants [42] or by dsRNA injection to C. elegans cells [27], although irregular nuclear processing of primary transcripts associated with PTGS/RNAi has been reported previously [43]. The FAD2-A1 intron-induced RNA silencing led to the understanding that RNA degradation can take place in the nucleus [44]. Although whether RNA degradation in the nucleus is inducible for other genes or in other plants has not been known, this phenomenon is intriguing because the involvement of nuclear events has been assumed for amplification of RNA silencing via transitivity [45] or intron-mediated suppression of RNA silencing [46].…”

Section: Mechanisms and Diverse Pathways Of Rna Silencingcontrasting

confidence: 53%

Gene Duplication and RNA Silencing in Soybean

Kasai¹,

Tsuchiya²,

Kanazawa³

2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

View full text Add to dashboard Cite

Section: Mechanisms and Diverse Pathways Of Rna Silencingcontrasting

confidence: 53%

Gene Duplication and RNA Silencing in Soybean

Kasai¹,

Tsuchiya²,

Kanazawa³

2013

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen Relationships

View full text Add to dashboard Cite

“…DCL4, which is primarily involved in the processing of perfectly complementary dsRNAs such as those comprising ihpRNA, resides exclusively in the nucleus (Kumakura et al 2009;Hoffer et al 2011;Jouannet et al 2012). In addition, intron sequences alone have been sufficient to trigger IR-PTGS, suggesting that not only ihpRNA processing but the entire process of IR-PTGS can be carried out in the nucleus as well (Hoffer et al 2011). Consistent with nuclear positioning of the RNAi machinery, extensive IR-PTGS in our transgenic plants considerably altered levels of endogenous sRNA produced in the nucleus.…”

Section: Discussionmentioning

confidence: 99%

Distinctive profiles of small RNA couple inverted repeat-induced post-transcriptional gene silencing with endogenous RNA silencing pathways in Arabidopsis

Wróblewski

Matvienko

Piskurewicz

et al. 2014

RNA

View full text Add to dashboard Cite

The experimental induction of RNA silencing in plants often involves expression of transgenes encoding inverted repeat (IR) sequences to produce abundant dsRNAs that are processed into small RNAs (sRNAs). These sRNAs are key mediators of posttranscriptional gene silencing (PTGS) and determine its specificity. Despite its application in agriculture and broad utility in plant research, the mechanism of IR-PTGS is incompletely understood. We generated four sets of 60 Arabidopsis plants, each containing IR transgenes expressing different configurations of uidA and CHALCONE SYNTHASE (At-CHS) gene fragments. Levels of PTGS were found to depend on the orientation and position of the fragment in the IR construct. Deep sequencing and mapping of sRNAs to corresponding transgene-derived and endogenous transcripts identified distinctive patterns of differential sRNA accumulation that revealed similarities among sRNAs associated with IR-PTGS and endogenous sRNAs linked to uncapped mRNA decay. Detailed analyses of poly-A cleavage products from At-CHS mRNA confirmed this hypothesis. We also found unexpected associations between sRNA accumulation and the presence of predicted open reading frames in the trigger sequence. In addition, strong IR-PTGS affected the prevalence of endogenous sRNAs, which has implications for the use of PTGS for experimental or applied purposes.

show abstract

“…Specific RISC members may also function in the nucleus. There is nuclear association of Ago1 and Ago2 with the progesterone receptor gene (3), Dicer is associated with nuclear ribosomal DNA chromatin, and posttranscriptional silencing can occur in nuclei (17). To date, however, NR coregulators have not been directly implicated in RISC activity or miRNA biogenesis and processing.…”

mentioning

confidence: 99%

RNA-induced silencing complex (RISC) Proteins PACT, TRBP, and Dicer are SRA binding nuclear receptor coregulators

Redfern

Colley

Beveridge

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

103

View full text Add to dashboard Cite

The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including protein kinase RNA activator (PACT), transactivation response RNA binding protein (TRBP), and Dicer, that process pre-microRNAs into mature microRNAs (miRNAs) that target specific mRNA species for regulation. There is increasing evidence for important functional interactions between the miRNA and nuclear receptor (NR) signaling networks, with recent data showing that estrogen, acting through the estrogen receptor, can modulate initial aspects of nuclear miRNA processing. Here, we show that the cytoplasmic RISC proteins PACT, TRBP, and Dicer are steroid receptor RNA activator (SRA) binding NR coregulators that target steroid-responsive promoters and regulate NR activity and downstream gene expression. Furthermore, each of the RISC proteins, together with Argonaute 2, associates with SRA and specific pre-microRNAs in both the nucleus and cytoplasm, providing evidence for links between NR-mediated transcription and some of the factors involved in miRNA processing.noncoding RNA | androgen action | prostate cancer M icroRNAs (miRNAs) are 22-nt noncoding RNAs that regulate gene expression by associating with the multiprotein RNA-induced silencing complex (RISC) and guiding RISC to silence specific mRNA species by mRNA degradation or translational inhibition (1). miRNAs are derived from large primary RNA transcripts, which are processed in the nucleus by Drosha/ Pasha into ∼70-nt precursor (pre-miRNA) duplexes. These duplexes are exported to the cytoplasm, and their conversion to mature 22-nt miRNAs is mediated by a transactivation response (TAR) RNA binding protein (TRBP)-Dicer complex (1), which provides a platform for RISC assembly and aids recruitment of Argonaute 2 (Ago2), the catalytic enzyme of RISC required for miRNA processing (2, 3). Protein kinase RNA (PKR) activator (PACT) is also integral to the RISC complex and associates with Dicer (4). TRBP and PACT are critical for efficient miRNA processing, because their depletion results in decreased mature miRNA levels and target gene silencing (2, 4, 5).Ligand-activated signaling by nuclear receptors (NRs) plays a key role in the promotion of human tumorigenesis. NR coregulators are a group of >300 molecules that act to fine tune ligand-induced gene transcription by acting as either coactivators [e.g., steroid receptor coactivator-1 (SRC-1)] or corepressors [e.g., nuclear receptor corepressor1 (NCoR1)] (6). One of the NR coregulators, steroid receptor RNA activator (SRA), is unique, because its RNA transcript functions to coactivate NR activity (7-9).Several links between miRNA pathways and NR action have been reported. miRNAs can regulate NR signaling at different levels, including targeting NRs [e.g., estrogen receptor-α (ERα)] (10, 11) or NR coregulator transcripts (e.g., amplified in breast cancer 1) (12). Conversely, NRs regulate an array of miRNAs in endocrine tissues (13). For example, ERα can regulate miRNA-21 expression at the transcriptional level (14) and the...

show abstract

Posttranscriptional gene silencing in nuclei

Cited by 83 publications

References 36 publications

Gene Duplication and RNA Silencing in Soybean

Gene Duplication and RNA Silencing in Soybean

Distinctive profiles of small RNA couple inverted repeat-induced post-transcriptional gene silencing with endogenous RNA silencing pathways in Arabidopsis

RNA-induced silencing complex (RISC) Proteins PACT, TRBP, and Dicer are SRA binding nuclear receptor coregulators

Contact Info

Product

Resources

About