Potassium assay in blastomere of two-cell mouse embryo after equilibration and washing from cryoprotector

Gol’dshtein, D. V.; Smolyaninova, E. I.; Pogorelov, A. G.

doi:10.1007/bf02694469

Cited by 2 publications

(1 citation statement)

References 2 publications

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“…The use of our method for making semithin (2-µ) sections of mouse embryo gave unique results for such promising cell technologies as cryopreservation of an early embryo [4,13] or therapeutic cloning [2,7].…”

Section: Group Sodium Potassiummentioning

confidence: 99%

Electron probe microanalysis of cytoplasmic concentrations of elements in a single cell in culture and suspension

Pogorelov

Goldstein

2006

Bull Exp Biol Med

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Electron probe microanalysis is a method for evaluation of concentrations of elements at the subcellular level, effective in studies of an individual cell in culture or suspension. Intracellular concentrations of cytoplasmic ions (K+, Na+, Cl-) most rapidly and markedly reacting to changes is an adequate criterion for evaluating the traumatism of manipulations used in cell technologies. Using electron probe microanalysis it will be possible to develop special procedures (for example, therapeutic cloning) for reprogramming or cryopreservation, when intactness of intracellular balance of element serves as the criterion of cell status.

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Section: Group Sodium Potassiummentioning

confidence: 99%

Electron probe microanalysis of cytoplasmic concentrations of elements in a single cell in culture and suspension

Pogorelov

Goldstein

2006

Bull Exp Biol Med

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High Potassium Concentration during Culturing of Early Mammalian Embryos: Normal or Extreme Situation?

2017

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Analysis of the element composition of oviduct and uterine fluid in mammals showed high potassium concentrations in the early embryo microenvironment in vivo. The results of early embryogenesis of mammals in vitro in the presence of high potassium concentrations are discussed. The data are summarized in accordance with the conditions of experimentally modeled pre-implantation development. Comparative assessment of the quality of embryo development until the blastocyst stage in vitro proved the embryos more successfully developed at potassium concentrations close to those registered in the oviductal fluid.

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Potassium assay in blastomere of two-cell mouse embryo after equilibration and washing from cryoprotector

Cited by 2 publications

References 2 publications

Electron probe microanalysis of cytoplasmic concentrations of elements in a single cell in culture and suspension

Electron probe microanalysis of cytoplasmic concentrations of elements in a single cell in culture and suspension

High Potassium Concentration during Culturing of Early Mammalian Embryos: Normal or Extreme Situation?

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