Potassium Channel Activation in Vascular Smooth Muscle

Siegel, G.; Emden, J.; Wenzel, Katharina; Mironneau, Jean; Stock, Günter

doi:10.1007/978-1-4615-3362-7_5

Cited by 61 publications

(43 citation statements)

References 18 publications

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“…3 . The coding region of rabK v1.3 was identified from the genomic clone by a combination of Southern blotting and DNA sequencing.…”

Section: Resultsmentioning

confidence: 99%

“…Phylogenetic tree reconstruction shows that rabK v1.3 is most closely related to the K v1. 3 Shaker family (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

“…These channels have been studied most extensively in excitable cells where they participate in diverse cellular functions such as action potential and pacemaker activity (1). Recently it has been appreciated that K v channels play a crucial role in the regulation of vascular smooth muscle contraction and, therefore, peripheral vascular resistance and blood pressure (2)(3)(4). In contrast, there are few reports of K v currents in epithelial cells (eye and type II pneumocytes) (5,6).…”

Section: Introductionmentioning

confidence: 99%

“…In this paper, we report the identification and molecular characterization of a novel K v channel, rabK v1. 3 , that is expressed in kidney medulla and in GRB-PAP1 cells.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning of a glibenclamide-sensitive, voltage-gated potassium channel expressed in rabbit kidney.

et al. 1996

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Shaker genes encode voltage-gated potassium channels (K v ). We have shown previously that genes from Shaker subfamilies K v1.1, 1.2, 1.4 are expressed in rabbit kidney. Recent functional and molecular evidence indicate that the predominant potassium conductance of the kidney medullary cell line GRB-PAP1 is composed of Shaker -like potassium channels. We now report the molecular cloning and functional expression of a new Shaker -related voltage-gated potassium channel, rabK v1.3 , that is expressed in rabbit brain and kidney medulla. The protein, predicted to be 513 amino acids long, is most closely related to the K v1.3 family although it differs significantly from other members of that family at the amino terminus. In Xenopus oocytes, rabK v1.3 cRNA expresses a voltage activated K current with kinetic characteristics similar to other members of the K v1.3 family. However, unlike previously described Shaker channels, it is sensitive to glibenclamide and its single channel conductance saturates. This is the first report of the functional expression of a voltage-gated K channel clone expressed in kidney. We conclude that rabK v1.3 is a novel member of the Shaker superfamily that may play an important role in renal potassium transport. ( J. Clin. Invest. 1996. 97:2525-2533.)

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“…3 . The coding region of rabK v1.3 was identified from the genomic clone by a combination of Southern blotting and DNA sequencing.…”

Section: Resultsmentioning

confidence: 99%

“…Phylogenetic tree reconstruction shows that rabK v1.3 is most closely related to the K v1. 3 Shaker family (Fig. 3).…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In this paper, we report the identification and molecular characterization of a novel K v channel, rabK v1. 3 , that is expressed in kidney medulla and in GRB-PAP1 cells.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning of a glibenclamide-sensitive, voltage-gated potassium channel expressed in rabbit kidney.

et al. 1996

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“…The present results demonstrate that EDHF/H 2 O 2 contributes substantially to the vasodilation during coronary autoregulation in vivo. Regarding the mechanism involved in the K Ca channel opening during coronary autoregulation, cellular acidosis 32 and increase in intracellular Ca 2ϩ concentration after ischemia 33 have been postulated to open K Ca channels as a compensatory mechanism after inhibition of NO synthesis. We have previously demonstrated that subendocardial arteriolar dilation during reactive hyperemia is more sensitive to L-NMMA than subepicardial arteriolar dilation.…”

Section: Role Of Edhf In Myocardial Ischemia During Coronary Autoregumentioning

confidence: 99%

Hydrogen Peroxide, an Endogenous Endothelium-Derived Hyperpolarizing Factor, Plays an Important Role in Coronary Autoregulation In Vivo

et al. 2003

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Background-Recent studies in vitro have demonstrated that endothelium-derived hydrogen peroxide (H 2 O 2 ) is an endothelium-derived hyperpolarizing factor (EDHF) in animals and humans. The aim of this study was to evaluate our hypothesis that endothelium-derived H 2 O 2 is an EDHF in vivo and plays an important role in coronary autoregulation. Methods and Results-To test this hypothesis, we evaluated vasodilator responses of canine (nϭ41) subepicardial small coronary arteries (Ն100 m) and arterioles (Ͻ100 m) with an intravital microscope in response to acetylcholine and to a stepwise reduction in coronary perfusion pressure (from 100 to 30 mm Hg) before and after inhibition of NO synthesis with N G -monomethyl-L-arginine (L-NMMA). After L-NMMA, the coronary vasodilator responses were attenuated primarily in small arteries, whereas combined infusion of L-NMMA plus catalase (an enzyme that selectively dismutates H 2 O 2 into water and oxygen) or tetraethylammonium (TEA, an inhibitor of large-conductance K Ca channels) attenuated the vasodilator responses of coronary arteries of both sizes. Residual arteriolar dilation after L-NMMA plus catalase or TEA was largely attenuated by 8-sulfophenyltheophylline, an adenosine receptor inhibitor. Conclusions-These results suggest that H 2 O 2 is an endogenous EDHF in vivo and plays an important role in coronary autoregulation in cooperation with NO and adenosine.

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