Potassium fluxes across the endoplasmic reticulum and their role in endoplasmic reticulum calcium homeostasis

Kuum, Malle; Veksler, Vladimir; Kaasik, Allen

doi:10.1016/j.ceca.2014.11.004

Cited by 36 publications

(42 citation statements)

References 106 publications

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“…The interaction shown suggests a linear increase in the phenolic content of the three genotypes in response to increased potassium level. Generally, the K + ion regulates the internal balance for the purpose of absorption and translocation of carbohydrates that indirectly affect the formation of the basic structures of phenylpropanoids (Kuum et al, 2015). However, it is not conclusive that the increase of the phenolic compounds content in the plant can always be linear with the increase of the application of K, therefore, it could be interesting to continue investigating the range of application of K, with the possibility of find a quadratic response to relate the level of K and the production of total phenolic compounds.…”

Section: Resultados Y Discusiónmentioning

confidence: 99%

“…Generalmente, el ion K + regula el equilibrio interno con el propósito de absorción y translocación de hidratos de carbono que afectan indirectamente la formación de las estructuras básicas de los fenilpropanoides (Kuum et al, 2015). Sin embargo, no es concluyente que el aumento del contenido de compuestos fenólicos en la planta pueda ser siempre lineal con el incremento de la aplicación de K, por lo tanto, podría ser interesante seguir investigando el rango de aplicación de K, con la posibilidad de encontrar una respuesta cuadrática para relacionar el nivel de K y la producción de compuestos fenólicos totales.…”

Section: Resultados Y Discusiónunclassified

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Efecto del potasio en el contenido fenólico y capacidad antioxidante de Ocimum basilicum L.

Martínez¹,

Salas-Pérez²,

Soto³

et al. 2017

Remexca

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ResumenEn la última década, se han realizado numerosos esfuerzos para incrementar el contenido de compuestos fenólicos y la capacidad antioxidante de plantas que vayan directo al consumo humano, empleando para esto diversos métodos de cultivo; sin embargo, los resultados han sido discrepantes acerca de la calidad de los frutos y el rendimiento obtenido, concluyendo que el alto contenido de compuestos fenólicos y la capacidad antioxidante no ha sido garantía de alto rendimiento de los cultivos. Por lo tanto, el objetivo de este estudio fue determinar un posible aumento del contenido en compuestos fenólicos totales, capacidad antioxidante total y rendimiento de tres genotipos de albahaca (Sweet Lemon, Red Rubin y Cinnamon) producidos bajo diferentes niveles de potasio (7, 9, 11 y 13 mM). Adicionalmente, se evaluó materia seca, altura de planta y número de hojas. El trabajo se llevó a cabo en la Comarca Lagunera de Durango en el año 2014. El análisis de varianza mostró diferencias significativas (p≤ 0.001) por efecto de genotipo y nivel de potasio en casi todas las variables. Se encontró interacción en compuestos fenólicos totales, capacidad antioxidante, rendimiento y altura de la planta. También se encontró correlación (R2) (p≤ 0.05) entre el nivel de potasio y todas AbstractIn the last decade, numerous efforts have been made to increase the content of phenolic compounds and the antioxidant capacity of plants that go directly to human consumption, using for this several methods of cultivation; however, the results have been discrepant about the quality of the fruits and the yield obtained, concluding that the high content of phenolic compounds and the antioxidant capacity has not been a guarantee of high crop yields. Therefore, the objective of this study was to determine a possible increase in total phenolic compounds, total antioxidant capacity and yield of three basil genotypes (Sweet Lemon, Red Rubin and Cinnamon) produced under different levels of potassium (7,9 , 11 and 13 mM). In addition, dry matter, plant height and number of leaves were evaluated. The work was carried out in the Comarca Lagunera of Durango in the year 2014. The analysis of variance showed significant differences (p≤ 0.001) due to genotype and potassium levels in almost all variables. It was found interaction in total phenolic compounds, antioxidant capacity, yield and height of the plant. The correlation (R2) (p≤ 0.05) was also found between the potassium level and all variables. The results indicate that las variables. Los resultados indican que el control de potasio en la solución de nutrientes puede ser una opción viable para mejorar las cualidades nutracéuticas en cuanto al contenido de compuestos fenólicos totales y la capacidad antioxidante sin afectar el rendimiento de albahaca.Palabras clave: Ocimum basilicum L., antioxidants, concentración de potasio, rendimiento.

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Section: Resultados Y Discusiónmentioning

confidence: 99%

Section: Resultados Y Discusiónunclassified

Efecto del potasio en el contenido fenólico y capacidad antioxidante de Ocimum basilicum L.

Martínez¹,

Salas-Pérez²,

Soto³

et al. 2017

Remexca

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“…These data demonstrate an intracellular residence of SK2 channels at the ER membrane of neuronal HT-22 cells, with potential importance for the CyPPA-mediated protective effects against ER stress. 13 To test this hypothesis in our model of ER stress, we applied quinine and propranolol, two known blockers of the ER membrane located K + /H + exchanger, 14 and identified significant inhibition of the protective effects of SK2 channel activation in ER stress-induced cell death (Figure 7g, Supplementary Figure S9). …”

Section: Resultsmentioning

confidence: 99%

“…11,12 Several ER-located potassium channels are supposed to be important for [Ca 2+ ] ER uptake and release by maintenance of the countercurrent required for electroneutrality. 13 For instance, small-conductance calcium-activated potassium (SK) channels have recently been detected in the sarco-/ endoplasmic reticulum of cardiomyocytes and neurons, respectively, where they may regulate [Ca 2+ ] ER uptake.…”

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confidence: 99%

Activation of SK2 channels preserves ER Ca2+ homeostasis and protects against ER stress-induced cell death

et al. 2015

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Alteration of endoplasmic reticulum (ER) Ca2+ homeostasis leads to excessive cytosolic Ca 2+ accumulation and delayed neuronal cell death in acute and chronic neurodegenerative disorders. While our recent studies established a protective role for SK channels against excessive intracellular Ca 2+ accumulation, their functional role in the ER has not been elucidated yet. We show here that SK2 channels are present in ER membranes of neuronal HT-22 cells, and that positive pharmacological modulation of SK2 channels with CyPPA protects against cell death induced by the ER stressors brefeldin A and tunicamycin. Calcium imaging of HT-22 neurons revealed that elevated cytosolic Ca 2+ levels and decreased ER Ca 2+ load during sustained ER stress could be largely prevented by SK2 channel activation. Interestingly, SK2 channel activation reduced the amount of the unfolded protein response transcription factor ATF4, but further enhanced the induction of CHOP. Using siRNA approaches we confirmed a detrimental role for ATF4 in ER stress, whereas CHOP regulation was dispensable for both, brefeldin A toxicity and CyPPA-mediated protection. Cell death induced by blocking Ca 2+ influx into the ER with the SERCA inhibitor thapsigargin was not prevented by CyPPA. Blocking the K + efflux via K + /H + exchangers with quinine inhibited CyPPA-mediated neuroprotection, suggesting an essential role of proton uptake and K + release in the SK channel-mediated neuroprotection. Our data demonstrate that ER SK2 channel activation preserves ER Ca 2+ uptake and retention which determines cell survival in conditions where sustained ER stress contributes to progressive neuronal death.

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“…Accurate measurement of cations like Li + , Na + , and K + is highly essential in various real environments such as environmental, industrial, clinical, and biological samples [1,6]. According to the literature, different analytical methods such as atomic absorption/emission spectrometry (AAS/AES) [7,8] inductively coupled plasma (ICP) [9], molecular/atomic fluorescence spectrometry (MFE/AFS) [10], ion-exchange chromatography [11] as well as electrochemical techniques like potentiometry using ionselective electrodes [12,13], voltammetry [14] and electro analysis [15] [16] have been reported.…”

Section: Introductionmentioning

confidence: 99%

Atomic Emission Spectroelectrochemistryas A Sensitive Technique for Trace and Ultra-Trace Determination of Metal Species

Doroodmand¹,

FatemehGhasemi²

2017

IJAEMS

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Potassium fluxes across the endoplasmic reticulum and their role in endoplasmic reticulum calcium homeostasis

Cited by 36 publications

References 106 publications

Efecto del potasio en el contenido fenólico y capacidad antioxidante de Ocimum basilicum L.

Efecto del potasio en el contenido fenólico y capacidad antioxidante de Ocimum basilicum L.

Activation of SK2 channels preserves ER Ca2+ homeostasis and protects against ER stress-induced cell death

Atomic Emission Spectroelectrochemistryas A Sensitive Technique for Trace and Ultra-Trace Determination of Metal Species

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