Potassium regulates the growth and toxin biosynthesis of Microcystis aeruginosa

He, Yan; Ma, Jing; Vanderwall, Joseph; Wei, Yanyan; Liu, Mengzi; Zhang, Zhaoxue; Li, Guo; He, Qiang; Li, Hong

doi:10.1016/j.envpol.2020.115576

Cited by 21 publications

(3 citation statements)

References 53 publications

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“…The synthesis of total MC-LR was significantly inhibited when exposed to 2-hydroxychalcone over a 48 h cultivation. Moreover, the positive correlation between MC-LR biosynthesis and photosynthetic activity in cyanobacteria has been proposed. , The inhibition of photosynthesis by 2-hydroxychalcone greatly reduced the production of ATP and negatively regulated the MC-LR biosynthesis, which jointly led to the reduced MC concentration in the solution. The release of MCs from M. aeruginosa could always be stimulated to resist oxidative damage under allelopathy algicides, despite inhibited cell growth. , As compared to these general algicides, 2-hydroxychalcone not only inhibited M. aeruginosa growth, but also decreased the MC production and release amounts to restrict secondary pollution.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the positive correlation between MC-LR biosynthesis and photosynthetic activity in cyanobacteria has been proposed. 56,57 The inhibition of photosynthesis by 2-hydroxychalcone greatly reduced the production of ATP and negatively regulated the MC-LR biosynthesis, which jointly led to the reduced MC concentration in the solution. The release of MCs from M. aeruginosa could always be stimulated to resist oxidative damage under allelopathy algicides, despite inhibited cell growth.…”

Section: T H I S C O N T E N T Imentioning

confidence: 99%

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2-Hydroxychalcone as a Novel Natural Photosynthesis Inhibitor against Bloom-Forming Cyanobacteria

Zhang

Yang

Huang

et al. 2022

J. Agric. Food Chem.

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The control of harmful cyanobacterial blooms has been becoming a global challenge. The development of eco-friendly algicides with strong specificity is urgently needed. The photosynthetic apparatus is a promising target site for algicides to minimize the possible harmful effects on animals and humans. In this study, biologically derived 2-hydroxychalcone efficiently inhibited the growth of bloom-forming M. aeruginosa by selectively interfering with photosynthesis. 2-Hydroxychalcone targeting Photosystem II (PSII) inhibited electron transfer between the primary and secondary electron acceptors (QA and QB) and the binding of plastoquinone (PQ) molecules to the QB binding pocket at the acceptor side of PSII, as revealed by polyphasic chlorophyll (Chl) a fluorescence induction and QA – reoxidation kinetics. Molecular docking for 2-hydroxychalcone to D1 protein and the proteomic responses of M. aeruginosa suggested that 2-hydroxychalcone formed a stable monodentate ligand with the nonheme iron in D1 protein, provoking significant modulation of PSII proteins. The unique binding mode of 2-hydroxychalcone with PSII differentiated it from classical PSII inhibitors. Furthermore, 2-hydroxychalcone down-regulated the expression of microcystin (MC) synthesis-related genes to restrain MC synthesis and release. These results indicated the potential application of 2-hydroxychalcone as an algicide or a template scaffold for designing novel derivatives with superior algicidal activity.

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Section: Resultsmentioning

confidence: 99%

Section: T H I S C O N T E N T Imentioning

confidence: 99%

2-Hydroxychalcone as a Novel Natural Photosynthesis Inhibitor against Bloom-Forming Cyanobacteria

Zhang

Yang

Huang

et al. 2022

J. Agric. Food Chem.

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“…First, according to our results, the protein encoded by mcyE , which regulates microcystin biosynthesis, was upregulated following CLA exposure, which would promote the biosynthesis of the microcystin (Table S5). Additionally, a large number of ABC transport proteins were upregulated, several of which are associated with microcystin transport and could enhance the release of cellular microcystins . Aspartic acid is involved in the production of microcystin-LR and showed nonsignificant change after single exposure but was significantly upregulated after repeated exposure (Table S7), indicating the induction of microcystin release, representing a potential risk in the aquatic environment that warrants further investigation .…”

Section: Discussionmentioning

confidence: 99%

Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa Versus Single Exposure through Photosynthesis, Oxidative Stress, and Energy Metabolism Shift

Mao,

Ye,

Yang

et al. 2024

Environ. Sci. Technol.

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Antibiotics are being increasingly detected in aquatic environments, and their potential ecological risk is of great concern. However, most antibiotic toxicity studies involve single-exposure experiments. Herein, we studied the effects and mechanisms of repeated versus single clarithromycin (CLA) exposure on Microcystis aeruginosa. The 96 h effective concentration of CLA was 13.37 μg/L upon single exposure but it reduced to 6.90 μg/L upon repeated exposure. Single-exposure CLA inhibited algal photosynthesis by disrupting energy absorption, dissipation and trapping, reaction center activation, and electron transport, thereby inducing oxidative stress and ultrastructural damage. In addition, CLA upregulated glycolysis, pyruvate metabolism, and the tricarboxylic acid cycle. Repeated exposure caused stronger inhibition of algal growth via altering photosynthetic pigments, reaction center subunits biosynthesis, and electron transport, thereby inducing more substantial oxidative damage. Furthermore, repeated exposure reduced carbohydrate utilization by blocking the pentose phosphate pathway, consequently altering the characteristics of extracellular polymeric substances and eventually impairing the defense mechanisms of M. aeruginosa. Risk quotients calculated from repeated exposure were higher than 1, indicating significant ecological risks. This study elucidated the strong influence of repeated antibiotic exposure on algae, providing new insight into antibiotic risk assessment.

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New insight on Fe-bioavailability: Bio-uptake, utilization and induce in optimizing methane production in anaerobic digestion

Zhang

et al. 2022

Chemical Engineering Journal

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Potassium regulates the growth and toxin biosynthesis of Microcystis aeruginosa

Cited by 21 publications

References 53 publications

2-Hydroxychalcone as a Novel Natural Photosynthesis Inhibitor against Bloom-Forming Cyanobacteria

2-Hydroxychalcone as a Novel Natural Photosynthesis Inhibitor against Bloom-Forming Cyanobacteria

Repeated Exposure Enhanced Toxicity of Clarithromycin on Microcystis aeruginosa Versus Single Exposure through Photosynthesis, Oxidative Stress, and Energy Metabolism Shift

New insight on Fe-bioavailability: Bio-uptake, utilization and induce in optimizing methane production in anaerobic digestion

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