Potato disease caused by combined infection with potato spindle tuber viroid and potato virus Y necrotic strain

Volkov, Yu. G.; Kakareka, N. N.; Pleshakova, T. I.; Kozlovskaya, Z. N.

doi:10.3103/s1068367407030081

Cited by 3 publications

(1 citation statement)

References 4 publications

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“…ВВКК распространился в посадках картофеля в Центральном, Северо-Западном и Дальневосточном округах России� -скои� Федерации (Kastalyeva et al, 1992;Owens et al, 2009). ВВКК был обнаружен более чем в 50% меристемнои� культуры in vitro (Kastalyeva et al, 1992), а также у некоторых сортов картофеля в Приморском крае на Дальнем Востоке (Romanova et al, 2007). R. A.…”

Section: диагностика ввкк методом отпцр (пцр с обратной транскрипцией...unclassified

<i>Solanum nigrum</i> L. is a potato spindle tuber viroid reservoir

Мироненко

Хютти

Kyrova

et al. 2022

Trudy po prikladnoy botanike, genetike i selektsii

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Background. Among the weeds of the Solanaceae family, a potential viroid infection reservoir, black nightshade (Solanum nigrum L.), is ubiquitous in Russia.Materials and methods. Molecular diagnostics of the viroid was performed using RT-PCR with the P3/P4 primers specific for PSTVd and 6Pospi F/R specific for the Pospiviroidae family of viroids. The amplification products were cloned and sequenced, and the nucleotide sequences were deposited in the GenBank database.Results. RT-PCR showed that 80–100% of plants were infected with PSTVd. The cDNA amplicons of two plants from Nizhny Novgorod (Sn1 and Sn2) and one plant from Tula Province (Sn3) were cloned (3 clones each) and sequenced. An analysis of the nucleotide variability in the clones revealed significant genetic differences between the PSTVd strains, independent of the geographical origin. Mutations U120C and U192C, compared with the reference strain VP35 (LC523658), were found in all 9 clones. The Sn1 isolate was close to the “potato” VP35 isolate and differed from it only by three mutations. The Sn2 isolate was represented by three identical clones and differed from VP35 by nine mutations, while Sn3 had 22 mutations in the region of 49–310 nucleotide positions in total for three clones. After artificial infection of healthy S. nigrum plants in a greenhouse, the presence of PSTVd in infected plants was confirmed and it was proved that the viroid was transmitted by seeds to plants of the next generation.Conclusion. For the first time in externally healthy plants of S. nigrum, widely distributed in the potato fields of Russia (Nizhny Novgorod and Tula Province), the potato spindle tuber viroid, PSTVd, was detected. It was proved that the viroid was preserved in the seeds of S. nigrum and transmitted to their progeny. Genetic heterogeneity of PSTVd strains from S. nigrum plants was found. Two mutations, U120C and U192C, were common for all tested clones of PSTVd strains.

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Section: диагностика ввкк методом отпцр (пцр с обратной транскрипцией...unclassified

<i>Solanum nigrum</i> L. is a potato spindle tuber viroid reservoir

Мироненко

Хютти

Kyrova

et al. 2022

Trudy po prikladnoy botanike, genetike i selektsii

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Genetic diversity and pathogenicity of potato spindle tuber viroid and chrysanthemum stunt viroid isolates in Russia

et al. 2021

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Possibilities of Obtaining and Controlling Virus-Free Material in the Process of Selection and Seed Production of Main Crops

Kakareka

Volkov

Tolkach

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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It is known that after being infected with phytoviruses plants do not recover. Breeding virus-resistant cultivars also does not give reliable results due to the high variability of pathogens and their diversity. And since it is impossible to obtain stable forms then one of the goals of protection a virus-free material and preventing its re-infection. To improve the health of vegetative propagated plant species, you can use the method of tissue culture of the apical meristem. In some cases true seeds can be healed by thermal or chemical treatment. The data on viruses infecting the main agricultural crops of the Russian Far East are presented; the features of their distribution and interaction with plants are described. Possible measures are given to prevent of phytoviruses spreading and re-infection of virus-free plants in the process of breeding and seed production, in gardening and landscape design.

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Potato disease caused by combined infection with potato spindle tuber viroid and potato virus Y necrotic strain

Cited by 3 publications

References 4 publications

<i>Solanum nigrum</i> L. is a potato spindle tuber viroid reservoir

<i>Solanum nigrum</i> L. is a potato spindle tuber viroid reservoir

Genetic diversity and pathogenicity of potato spindle tuber viroid and chrysanthemum stunt viroid isolates in Russia

Possibilities of Obtaining and Controlling Virus-Free Material in the Process of Selection and Seed Production of Main Crops

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