Potato E3 ubiquitin ligase PUB27 negatively regulates drought tolerance by mediating stomatal movement

Tang, Xun; Ghimire, Shantwana; Liu, Weigang; Fu, Xue; Zhang, Huanhuan; Zhang, Ning; Si, Hongyu

doi:10.1016/j.plaphy.2020.07.026

Cited by 9 publications

(8 citation statements)

References 33 publications

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“…For instance, PUB11, PUB22 and PUB23 negatively regulate drought tolerance by degrading receptor-like protein kinases and ABA receptors in Arabidopsis [56,57]. Moreover, PUB27 in potatoes negatively regulates drought tolerance by mediating stomatal movement [58].…”

Section: Discussionmentioning

confidence: 99%

Comparative physiological and coexpression network analyses reveal the potential drought tolerance mechanism of peanut

et al. 2022

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Background Drought stress has negative effects on plant growth and productivity. In this study, a comprehensive analysis of physiological responses and gene expression was performed. The responses and expressions were compared between drought-tolerant (DT) and drought-sensitive (DS) peanut varieties to investigate the regulatory mechanisms and hub genes involved in the impact of drought stress on culture. Results The drought-tolerant variety had robust antioxidative capacities with higher total antioxidant capacity and flavonoid contents, and it enhanced osmotic adjustment substance accumulation to adapt to drought conditions. KEGG analysis of differentially expressed genes demonstrated that photosynthesis was strongly affected by drought stress, especially in the drought-sensitive variety, which was consistent with the more severe suppression of photosynthesis. The hub genes in the key modules related to the drought response, including genes encoding protein kinase, E3 ubiquitin-protein ligase, potassium transporter, pentatricopeptide repeat-containing protein, and aspartic proteinase, were identified through a comprehensive combined analysis of genes and physiological traits using weighted gene co-expression network analysis. There were notably differentially expressed genes between the two varieties, suggesting the positive roles of these genes in peanut drought tolerance. Conclusion A comprehensive analysis of physiological traits and relevant genes was conducted on peanuts with different drought tolerances. The findings revealed diverse drought-response mechanisms and identified candidate genes for further research.

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Section: Discussionmentioning

confidence: 99%

Comparative physiological and coexpression network analyses reveal the potential drought tolerance mechanism of peanut

et al. 2022

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“…In previous studies, many PUBs have been reported to be involved in various abiotic stress responses. Among them, class IV PUBs, which possess C‐terminal ARM repeats, have been known to be key participants in abiotic stress response (Mao et al, 2022, Trenner et al, 2022, Cho et al, 2006, Cho et al, 2008, Bergler and Hoth, 2011, Liu et al, 2011, Hye Jo et al, 2016, Zhang et al, 2017, Zhao et al, 2017, Wang et al, 2019, Tang et al, 2020, Ahn et al, 2022, Lv et al, 2022, Saini et al, 2023). Although several PUBs containing a leucine‐rich domain in the C‐terminus and members of the class IV PUBs have been functionally characterized in Arabidopsis (Zhang et al, 2017, Zhao et al, 2017, Wang et al, 2019, Tang et al, 2020), not much has been elucidated in other species.…”

Section: Resultsmentioning

confidence: 99%

“…Among them, class IV PUBs, which possess C‐terminal ARM repeats, have been known to be key participants in abiotic stress response (Mao et al, 2022, Trenner et al, 2022, Cho et al, 2006, Cho et al, 2008, Bergler and Hoth, 2011, Liu et al, 2011, Hye Jo et al, 2016, Zhang et al, 2017, Zhao et al, 2017, Wang et al, 2019, Tang et al, 2020, Ahn et al, 2022, Lv et al, 2022, Saini et al, 2023). Although several PUBs containing a leucine‐rich domain in the C‐terminus and members of the class IV PUBs have been functionally characterized in Arabidopsis (Zhang et al, 2017, Zhao et al, 2017, Wang et al, 2019, Tang et al, 2020), not much has been elucidated in other species. In this regard, we speculated whether leucine‐rich domain‐containing PUBs may also have a functional role in abiotic stress response in Capsicum annuum .…”

Section: Resultsmentioning

confidence: 99%

“…Ubiquitination is an important issue in plant stress response, and various PUBs have been reported to be associated with abiotic stress responses in plants (Cho et al, 2008, Bergler and Hoth, 2011, Liu et al, 2011, Seo et al, 2012, Hye Jo et al, 2016, Wang et al, 2016, Zhang et al, 2017, Zhao et al, 2017, Wang et al, 2019, Tang et al, 2020, Chen et al, 2021, Wang et al, 2022). Functional analyses of PUBs have mainly been performed in Arabidopsis, while their characterization in pepper remains limited.…”

Section: Discussionmentioning

confidence: 99%

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Pepper U‐Box E3 ubiquitin ligase 24, CaPUB24, negatively regulates drought stress response

Bae,

Cho,

Choi

et al. 2024

Physiologia Plantarum

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Under stress conditions, plants modulate their internal states and initiate various defence mechanisms to survive. The ubiquitin‐proteasome system is one of the critical modules in these mechanisms, and Plant U‐Box proteins play an important role in this process as E3 ubiquitin ligases. Here, we isolated the Plant U‐box 24 gene CaPUB24 (Capsicum annuum Plant U‐Box 24) from pepper and characterized its functions in response to drought stress. We found that, compared to the other CaPUBs in the same group, the expression of CaPUB24 was significantly induced by drought stress. We also found that CaPUB24 was localized to the nucleus and cytoplasm and had E3 ubiquitin ligase activity. To investigate the biological role of CaPUB24 in response to drought stress further, we generated CaPUB24‐silenced pepper plants and CaPUB24‐overexpressing Arabidopsis transgenic plants. CaPUB24‐silenced pepper plants exhibited enhanced drought tolerance compared to the control plants due to reduced transpirational water loss and increased abscisic acid (ABA) sensitivity. In contrast, CaPUB24‐overexpressing Arabidopsis transgenic plants exhibited reduced drought tolerance and ABA‐insensitive phenotypes. Our findings suggest that CaPUB24 negatively modulates drought stress response in an ABA‐dependent manner.

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“…Soltu09G016850 was clustered with AtPUB46 and AtPUB48 , up-regulated under salt and drought stress in DM, indicating Soltu09G016850 may also be related to abiotic stress responses in potato. Tang et al [ 63 ] found that StPUB27 may negatively regulate the tolerance of transgenic potato to drought by mediating stomatal conductance. According to the blastn analysis, the StPUB27 found by Tang et al was Soltu03G028100 , which only differentially expressed in DM, but not differentially expressed in tetraploid potato under abiotic stress.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of the U-box E3 ligases gene family in potato (Solanum tuberosum L.) and overexpress StPUB25 enhance drought tolerance in transgenic Arabidopsis

Liu,

Wang,

et al. 2024

BMC Genomics

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Background Plant U-box (PUB) E3 ubiquitin ligases have vital effects on various biological processes. Therefore, a comprehensive and systematic identification of the members of the U-box gene family in potato will help to understand the evolution and function of U-box E3 ubiquitin ligases in plants. Results This work identified altogether 74 PUBs in the potato (StPUBs) and examined their gene structures, chromosomal distributions, and conserved motifs. There were seventy-four StPUB genes on ten chromosomes with diverse densities. As revealed by phylogenetic analysis on PUBs within potato, Arabidopsis, tomato (Solanum lycopersicum), cabbage (Brassica oleracea), rice (Oryza sativa), and corn (Zea mays), were clustered into eight subclasses (C1-C8). According to synteny analysis, there were 40 orthologous StPUB genes to Arabidopsis, 58 to tomato, 28 to cabbage, 7 to rice, and 8 to corn. In addition, RNA-seq data downloaded from PGSC were utilized to reveal StPUBs’ abiotic stress responses and tissue-specific expression in the doubled-monoploid potato (DM). Inaddition, we performed RNA-seq on the ‘Atlantic’ (drought-sensitive cultivar, DS) and the ‘Qingshu NO.9’ (drought-tolerant cultivar, DT) in early flowering, full-blooming, along with flower-falling stages to detect genes that might be involved in response to drought stress. Finally, quantitative real-time PCR (qPCR) was carried out to analyze three candidate genes for their expression levels within 100 mM NaCl- and 10% PEG 6000 (w/v)-treated potato plantlets for a 24-h period. Furthermore, we analyzed the drought tolerance of StPUB25 transgenic plants and found that overexpression of StPUB25 significantly increased peroxidase (POD) activity, reduced ROS (reactive oxygen species) and MDA (malondialdehyde) accumulation compared with wild-type (WT) plants, and enhancing drought tolerance of the transgenic plants. Conclusion In this study, three candidate genes related to drought tolerance in potato were excavated, and the function of StPUB25 under drought stress was verified. These results should provide valuable information to understand the potato StPUB gene family and investigate the molecular mechanisms of StPUBs regulating potato drought tolerance.

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Potato E3 ubiquitin ligase PUB27 negatively regulates drought tolerance by mediating stomatal movement

Cited by 9 publications

References 33 publications

Comparative physiological and coexpression network analyses reveal the potential drought tolerance mechanism of peanut

Comparative physiological and coexpression network analyses reveal the potential drought tolerance mechanism of peanut

Pepper U‐Box E3 ubiquitin ligase 24, CaPUB24, negatively regulates drought stress response

Genome-wide analysis of the U-box E3 ligases gene family in potato (Solanum tuberosum L.) and overexpress StPUB25 enhance drought tolerance in transgenic Arabidopsis

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