Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities

Lojan, Paul; Senés‐Guerrero, Carolina; Suárez, Juan Pablo; Kromann, Peter; Schüßler, Arthur; Declerck, Stéphane

doi:10.1007/s13199-016-0471-2

Cited by 35 publications

(30 citation statements)

References 49 publications

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“…Acaulospora has been classified as stress‐tolerant (Chagnon, Bradley, Maherali, & Klironomos, ). Indeed, it was frequently reported under harsh climatic conditions in acidic soils (i.e., pH 3.6–4.20 (Morton, )) as well as under high elevation sites such as the Andes region 2500 m asl (Loján et al., ). In our study, this genus was observed in alkaline soils (pH 8) as well as at lower pH (5–6) at 300 m asl, in highly hydrocarbon‐polluted conditions, demonstrating its ability to develop under highly contrasting conditions.…”

Section: Discussionmentioning

confidence: 99%

“…High-throughput 454-pyrosequencing of an ~800 bp rDNA fragment was conducted and analyzed, using a reference "phylogenetic backbone" based on long AMF sequences (i.e., SSU-ITS-LSU 1.5 kb fragment) (Krüger, Krüger, Walker, Stockinger, & Schüßler, 2012;Stockinger et al, 2010) and an evolutionary placement algorithm (EPA), which allows the phylogenetic annotation of sequences to the species level (Senés-Guerrero & Schüßler, 2016a). The AMF community composition in plant roots was evaluated according to the site of collection within the Pond by comparing the relative read abundance (RA) of AMF species (Loján et al, 2017;Senés-Guerrero & Schüßler, 2016a).…”

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confidence: 99%

“…The branches of the phylogenetic backbone tree show the placement of the short sequences by EPA. To allow comparisons, species were annotated with the same species numbers as used in previous studies(Loján et al, 2017;Senés-Guerrero & Schüßler, 2016a;Senés-Guerrero, Torres-Cortés, Pfeiffer, Rojas, & Schüßler, 2014).…”

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confidence: 99%

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Community composition of arbuscular mycorrhizal fungi associated with native plants growing in a petroleum‐polluted soil of the Amazon region of Ecuador

et al. 2018

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Arbuscular mycorrhizal fungi (AMF) are worldwide distributed plant symbionts. However, their occurrence in hydrocarbon-polluted environments is less investigated, although specific communities may be present with possible interest for remediation strategies. Here, we investigated the AMF community composition associated with the roots of diverse plant species naturally recolonizing a weathered crude oil pond in the Amazon region of Ecuador. Next generation 454 GS-Junior sequencing of an 800 bp LSU rRNA gene PCR amplicon was used. PCR amplicons were affiliated to a maximum-likelihood phylogenetic tree computed from 1.5 kb AMF reference sequences. A high throughput phylogenetic annotation approach, using an evolutionary placement algorithm (EPA) allowed the characterization of sequences to the species level. Fifteen species were detected. Acaulospora species were identified as dominant colonizers, with 73% of relative read abundance, Archaeospora (19.6%) and several genera from the Glomeraceae (Rhizophagus, Glomus macrocarpum-like, Sclerocystis, Dominikia and Kamienskia) were also detected. Although, a diverse community belonging to Glomeraceae was revealed, they represented <10% of the relative abundance in the Pond. Seventy five % of the species could not be identified, suggesting possible new species associated with roots of plants under highly hydrocarbon-polluted conditions.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Community composition of arbuscular mycorrhizal fungi associated with native plants growing in a petroleum‐polluted soil of the Amazon region of Ecuador

et al. 2018

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“…The performance of commercial products has been variable (e.g. Loj an et al, 2017) and they are thus little used (Tarbell & Koske, 2007;Faye et al, 2013). More broadly, although inoculation has the potential to increase growth and P uptake, particularly under low soil P availability (e.g.…”

Section: Augmentation With Indigenous Amf May Benefit Cropsmentioning

confidence: 99%

Little evidence that farmers should consider abundance or diversity of arbuscular mycorrhizal fungi when managing crops

2018

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Contents Summary 1092 I. Introduction 1093 II. Investigating activity of AMF in agroecosystems 1093 III. Crop benefit from AMF: agronomic and mycorrhizal literature differ 1094 IV. Flawed methodology leads to benefits of mycorrhizas being overstated 1094 V. Rigorous methodology suggests low colonisation by AMF can sometimes reduce crop yield 1095 VI. Predicting when mycorrhizas matter for crop yield 1096 VII. Crop genotype 1099 VIII. Fungal genotype 1100 IX. Complex interactions between the mycorrhizal fungal and soil microbial communities 1102 X. Phosphorus-efficient agroecosystems 1102 XI. Conclusions 1103 Acknowledgements 1104 References 1104 SUMMARY: Arbuscular mycorrhizal fungi (AMF) are ubiquitous in agroecosystems and often stated to be critical for crop yield and agroecosystem sustainability. However, should farmers modify management to enhance the abundance and diversity of AMF? We address this question with a focus on field experiments that manipulated colonisation by indigenous AMF and report crop yield, or investigated community structure and diversity of AMF. We find that the literature presents an overly optimistic view of the importance of AMF in crop yield due, in part, to flawed methodology in field experiments. A small body of rigorous research only sometimes reports a positive impact of high colonisation on crop yield, even under phosphorus limitation. We suggest that studies vary due to the interaction of environment and genotype (crop and mycorrhizal fungal). We also find that the literature can be overly pessimistic about the impact of some common agricultural practices on mycorrhizal fungal communities and that interactions between AMF and soil microbes are complex and poorly understood. We provide a template for future field experiments and a list of research priorities, including phosphorus-efficient agroecosystems. However, we conclude that management of AMF by farmers will not be warranted until benefits are demonstrated at the field scale under prescribed agronomic management.

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“…The positive effect of AM on the growth of potato, such as increased tuber yield and total biomass, has been reported both in greenhouse (Davies et al, 2005) and in field scale (Douds et al, 2007;Hijri, 2015). However, the beneficial effect of AM on potatoes may be decreased by many factors, such as low AM abundance caused by poor inoculum establishment (Lojá n et al, 2017). The research of the interactive effect of biochar and AM on the potato growth is still limited.…”

Section: Introductionmentioning

confidence: 99%

Nutrient uptake and growth of potato: Arbuscular mycorrhiza symbiosis interacts with quality and quantity of amended biochars

Yang

Ravnskov

Andersen

2020

J. Plant Nutr. Soil Sci.

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Aims: The aim of this study was to explore interactive effects between quality (types) and quantity (application rates) of biochar as well as of arbuscular mycorrhiza (AM) symbiosis on the growth of potato plants. Methods: A low P sandy loam soil was amended with 0%, 1.5%, or 2.5% (w/w) of either of 4 types of biochar, which were produced from wheat straw pellets (WSP) or miscanthus straw pellets (MSP) pyrolyzed at temperatures of either 550°C or 700°C. Potato plants grown in pots containing the soils or soil biochar mixture were inoculated with or without AM fungus (AMF), Rhizophagus irregularis. The experiment was carried out under fully irrigated semi‐field conditions and plants were harvested 101 days after planting. Results: Application of high temperature biochar decreased growth, biomass and tuber yield of potato plants, while the low temperature biochar had a similar effect on yield as plants grown without biochar amendment. Total biomass of potato plants were decreased with the increasing rate of biochar. Arbuscular mycorrhizal fungus inoculation stimulated the growth of potato plants in all organs, increased tuber biomass significantly in 1.5% MSP700 amended plants, and to a lesser degree for WSP700, MSP550, and WSP550. In addition, plant biomass gain was linearly related to N, P, and K uptake, the ratio of P to N in the leaf of plants indicated that all treatments were mainly P‐limited. A multiple linear regression using P uptake and biochar rate as independent variables explained 91% of the variation in total biomass. The single effect of AMF inoculation, type and rate of biochar affected plant N, P and K uptake similarly. While AMF inoculation significantly increased P uptake in potato plants grown in soil with WSP700 or MSP700 despite of the rate of biochar. In general, application of biochar significantly increased AMF root colonization of potato plants. Conclusions: The application of MSP550 at 1.5% combined with AMF stimulated growth of potato the most. Furthermore, the results indicated that the interactive effect of AMF inoculation, biochar type and application rate on potato growth to a large extent could be explained by effects on plant nutrient uptake.

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Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities

Cited by 35 publications

References 49 publications

Community composition of arbuscular mycorrhizal fungi associated with native plants growing in a petroleum‐polluted soil of the Amazon region of Ecuador

Community composition of arbuscular mycorrhizal fungi associated with native plants growing in a petroleum‐polluted soil of the Amazon region of Ecuador

Little evidence that farmers should consider abundance or diversity of arbuscular mycorrhizal fungi when managing crops

Nutrient uptake and growth of potato: Arbuscular mycorrhiza symbiosis interacts with quality and quantity of amended biochars

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