Potato Leaf Roll Virus 3' Genome Organization: Sequence of the Coat Protein Gene and Identification of a Viral Subgenomic RNA

Smith, O. P.; Harris, Kerry F.

doi:10.1094/phyto-80-609

Cited by 20 publications

(17 citation statements)

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“…Previous size estimates have been in the region of 2-5 kb (Smith & Harris, 1990), although Tacke et al (1990) obtained a value of 2.3 kb by locating the 5'-terminal nucleotide 40 residues upstream of the coat protein initiation codon. However, our results using a Scottish isolate of PLRV suggest a larger molecular size and a 5' origin further upstream.…”

Section: Introductionmentioning

confidence: 99%

The location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mRNA

Miller¹,

Mayo²

1991

Journal of General Virology

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Section: Introductionmentioning

confidence: 99%

The location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mRNA

Miller¹,

Mayo²

1991

Journal of General Virology

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“…This RNA is not encapsidated into viral particles (Smith & Harris, 1990) and contains the gene for the coat protein (CP, Tacke et al, 1989), which is separated by an amber stop codon from the ORF for a 56K protein (Fig. I a).…”

mentioning

confidence: 99%

The Potato Leafroll Luteovirus 17K Protein is a Single-stranded Nucleic Acid-binding Protein

Tacke

Prüfer

Schmitz

et al. 1991

Journal of General Virology

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“…The ORF 3'-proximal to the NY-RPV coat protein ORF is proposed to be expressed by readthrough of the coat protein gene termination codon, as with a similar ORF of Vic-PAV and, indeed, with luteoviruses in general. A conserved 14 nucleotide sequence surrounding the luteovirus 22K coat protein gene termination codon has been identified previously, and a role for this sequence in the expression of the readthrough ORF postulated (Smith & Harris, 1990;Veidt et al, 1988). An examination of this region from NY-RPV indicates that this conserved region is actually the 12 nucleotide sequence AAAUAGGUAGAC, with the coat protein gene termination codon indicated by the underlined nucleotides.…”

Section: Comparison With Other Luteovirusesmentioning

confidence: 99%

Nucleotide sequence analysis and genomic organization of the NY-RPV isolate of barley yellow dwarf virus

Vincent

Lister

1991

Journal of General Virology

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cDNA clones representing the ssRNA genome of the NY-RPV isolate of barley yellow dwarf luteovirus (BYDV) were sequenced and 5600 nucleotides of the genome were determined. The deduced genome organization has limited similarity to that of another BYDV isolate, Vic-PAV, but is identical to that of beet western yellows (BWYV) and potato leafroll (PLRV) luteoviruses. NY-RPV has six major positive-sense open reading frames (ORFs) and, by comparison with RNA-dependent RNA polymerase and nucleic acid helicase consensus sequence motifs, it is postulated that NY-RPV ORF2 and ORF3 encode the viral replicase, which is expressed by a translational frameshift mechanism. The region of the NY-RPV genome containing the 22K coat protein ORF, the apparently associated internal apparent VPg ORF and the ORF immediately 3'-proximal (ORF6) to the coat protein ORF are organized as reported for other luteoviruses. Evidence is presented showing that ORF6 is expressed by readthrough of the coat protein gene termination codon, and that this protein is associated with the intact virus as a 65K protein. Although NY-RPV infects graminaceous rather than dicotyledonous plants, the taxonomic relationships between BYDV isolates and other luteoviruses deduced from the genome organization and sequence data strongly suggest that NY-RPV is distinct from the PAV-like isolates of BYDV and is more closely related to BWYV and PLRV.

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Potato Leaf Roll Virus 3' Genome Organization: Sequence of the Coat Protein Gene and Identification of a Viral Subgenomic RNA

Cited by 20 publications

References 0 publications

The location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mRNA

The location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mRNA

The Potato Leafroll Luteovirus 17K Protein is a Single-stranded Nucleic Acid-binding Protein

Nucleotide sequence analysis and genomic organization of the NY-RPV isolate of barley yellow dwarf virus

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