Potato R1 resistance gene confers resistance against Phytophthora infestans in transgenic tomato plants

Abstract

Tomato is challenged by several pathogens which cause loss of production. One such pathogen is the oomycete Phytophthora infestans which is able to attack all the aerial parts of the plant. Although a wide range of resistance sources are available, genetic control of this disease is not yet successful. Pyramiding R-genes through genetic transformation could be a straightforward way to produce tomato and potato lines carrying durable resistance to P. infestans. In this work the R1 potato gene was transferred into tomato lines. The tomato transgenic lines were analyzed by using q-RT-PCR and progeny segregation to determine the gene copy number. To test the hypothesis that R1 represents a specifically regulated R-gene, transgenic tomato plants were inoculated with P. infestans isolate 88133 and IPO. All the plants containing the R1 gene were resistant to the late blight isolate IPO-0 and susceptible to isolate 88133. These results provide evidence for specific activation of the R1 gene during pathogen challenge. Furthermore, evidence for enhancement of PR-1 gene expression during P. infestans resistance response was obtained.

Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection

“…It differs from their N-terminal which should be a leucine zipper domain (LZ) or a Toll/interleukin-1-receptor homology region (TIR). The potato R1 gene belonging to the LZ/NBS/LRR class has been cloned and its functionality has been verified in transgenic tomato [14]. The resistance conferred by R genes is very efficient, but also specific to a particular strain and might be avoided by a mutant or a new strain.…”

Plant Roots Release Small Extracellular Vesicles with Antifungal Activity

“…A high proportion of the identified proteins (23 out of 100) are known to participate in plant defense responses ( Table 2 ). Several of them are involved in recognition and signal transduction, such as three receptor-like serine/threonine-protein kinases and two putative late blight resistance proteins (R1A-10 and R1A-3) [ 24 , 25 ]. The presence of these receptors, alongside with calmodulin 5 and NDR1/HIN1-like protein, associated to biotic stress signaling, may indicate that root-released EVs could participate in pathogen perception and activation of the immune response that restricts pathogen growth.…”

A New Active Substance Derived from Lyzed Willaertia magna C2c Maky Cells to Fight Grapevine Downy Mildew

“…PR1 proteins belong to the most important group of PR proteins induced by a biotic or abiotic stress. They have shown antifungal activity, especially against oomycetes such as Phytophthora infestans on tomato plants [ 35 ], and Peronospora species. Their mode of action is related to their sterol-binding activity [ 36 ] and suppression of cell death-dependent disease symptoms [ 37 ].…”