Potato (Solanum tuberosum L.) non-specific lipid transfer protein StLTP6 promotes viral infection by inhibiting virus-induced RNA silencing

Shang, Kaijie; Xu, Yang; Cao, Weilin; Xie, Xiaolin; Zhang, Yanru; Zhang, Jingfeng; Li, Hongmei; Zhou, Shumei; Zhu, Xiaoping; Zhu, Changxiang

doi:10.1007/s00425-022-03948-6

Cited by 11 publications

(9 citation statements)

References 58 publications

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“…For instance, NbLTP1 (homolog of ShnsLTPI.19 ) from N. benthamiana contributed to accumulation of bamboo mosaic virus that possibly resulted from the regulation of lipid-binding activity of NbLTP1 by calmodulin binding or phosphorylation ( Chiu et al, 2020 ). In potato, StLTP6 (homolog of ShnsLTPI.20 ) promoted viral infection by potato virus Y and S by inhibiting expression of multiple genes involved in the RNA silencing pathway ( Shang et al, 2022 ). Interestingly, GhnsLTPsA10 from cotton ( G. hirsutum ) negatively mediated insect (aphid and bollworm) resistance, yet positively enhanced resistance to fungi ( Verticillium dahliae and Fusarium oxysporum f. sp.…”

Section: Discussionmentioning

confidence: 99%

“…The StLTP10 gene acts as a positive regulator for resistance of potato ( Solanum tuberosum ) to Phytophthora infestans ( Wang et al, 2021 ). NbLTP1 from Nicotiana benthamiana promotes bamboo mosaic virus accumulation ( Chiu et al, 2020 ) and StLTP6 from potato facilitated viral infection ( Shang et al, 2022 ). GhnsLTPsA10 from cotton ( Gossypium hirsutum ) positively enhanced resistance to fungi, but negatively mediates insect resistance in transgenic Arabidopsis and cotton with gene silencing ( Chen et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Systematic and functional analysis of non-specific lipid transfer protein family genes in sugarcane under Xanthomonas albilineans infection and salicylic acid treatment

Zhao²,

Shi³

et al. 2022

Front. Plant Sci.

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Plant non-specific lipid transfer proteins (nsLTPs) are small basic proteins that play a significant regulatory role in a wide range of physiological processes. To date, no genome-wide survey and expression analysis of this gene family in sugarcane has been performed. In this study we identified the nsLTP gene family in Saccharum spontaneum and carried out expression profiling of nsLTPs in two sugarcane cultivars (Saccharum spp.) that have different resistance to leaf scald caused by Xanthomonas albilineans (Xa) infection. The effect of stress related to exogenous salicylic acid (SA) treatment was also examined. At a genome-wide level, S. spontaneum AP85-441 had 71 SsnsLTP genes including 66 alleles. Tandem (9 gene pairs) and segmental (36 gene pairs) duplication events contributed to SsnsLTP gene family expansion. Five SsnsLTP proteins were predicted to interact with five other proteins. Expression of ShnsLTPI.8/10/Gb.1 genes was significantly upregulated in LCP85-384 (resistant cultivar), but downregulated in ROC20 (susceptible cultivar), suggesting that these genes play a positive regulatory role in response of sugarcane to Xa infection. Conversely, ShnsLTPGa.4/Ge.3 appears to act as a negative regulator in response Xa infection. The majority (16/17) of tested genes were positively induced in LCP85-384 72 h after SA treatment. In both cultivars, but particularly in LCP85-384, ShnsLTPIV.3/VIII.1 genes were upregulated at all time-points, suggesting that the two genes might act as positive regulators under SA stress. Meanwhile, both cultivars showed downregulated ShnsLTPGb.1 gene expression, indicating its potential negative role in SA treatment responses. Notably, the ShnsLTPGb.1 gene had contrasting effects, with positive regulation of gene expression in response to Xa infection and negative regulation induced by SA stress. Together, our results provide valuable information for elucidating the function of ShnsLTP family members under two stressors and identified novel gene sources for development of sugarcane that are tolerant of environmental stimuli.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Systematic and functional analysis of non-specific lipid transfer protein family genes in sugarcane under Xanthomonas albilineans infection and salicylic acid treatment

Zhao²,

Shi³

et al. 2022

Front. Plant Sci.

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“…In accordance with the method of our laboratory (Shang et al, 2022), the Agrobacterium was suspended in osmotic buffer (10 mM MgCl 2 , 10 mM MES [pH 5.6] and 100 μM acetosyringone), and the OD600 of single bacteria was adjusted to 0.5 before infiltration. A needleless syringe was used to penetrate the paraxial side of the four-week-old N. benthamina leaves.…”

Section: Agrobacterium Infiltration and Virus Inoculationmentioning

confidence: 99%

“…In accordance with the method of our laboratory (Shang et al, 2022), 0.5 g of infiltrated leaves of N. benthamiana was fully ground in a liquid nitrogen mortar, 1 ml of Trizol (Invitrogen, Shanghai, China) was added to shake and mix, chloroform was used for extraction, 75% ethanol was used for rinsing after centrifugal separation, and ddH 2 O was used for dissolution to obtain an RNA solution. Reverse transcription of cDNA was performed using a reverse transcription kit (Vazyme, Nanjing, China, R333).…”

Section: Rna Extraction and Qrt-pcr Assaymentioning

confidence: 99%

Tomato chlorosis virus p22 interacts with NbBAG5 to inhibit autophagy and regulate virus infection

Shang

Xiao

Zhang

et al. 2022

Preprint

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Tomato chlorosis virus (ToCV) is a member of the genus Crinivirus in the family Closteroviridae. It has a wide host range and wide distribution, causing serious harm to the vegetable industry. The autophagy pathway plays an important role in plant resistance to virus infection. Viruses and plant hosts coevolve in defence and antidefence processes around autophagy. In this study, ToCV p22 protein was selected to verify the interaction between ToCV p22 and NbBAG5. Through overexpression and downregulation of NbBAG5, it was found that NbBAG5 could negatively regulate ToCV infection. NbBAG5 was found to be localised in mitochondria and can change the original localisation of ToCV p22, which is colocalised in mitochondria. Further study found that NbBAG5 inhibited the expression of mitophagy-related genes and the number of autophagosomes, thereby regulating viral infection by affecting mitophagy. In summary, this study demonstrated that ToCV p22 affects autophagy by interacting with NbBAG5, established the association between virus infection, BAG proteins family and autophagy pathway and explained the molecular mechanism by which ToCV p22 interacts with NbBAG5 to inhibit autophagy to regulate viral infection.

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“…LTPs were first observed in spinach ( Spinacea oleracea L.) leaves, maize ( Zea mays L.) coleoptiles, and barley ( Hordeum vulgare L.) aleurone (Wei & Zhong, 2014 ). It was reported that the LTP genes were highly adaptable to stress in maize (Noonan et al, 2017 ), rice ( Oryza sativa L.) (Lin et al, 2017 ), cotton ( Gossypium hirsutum L.) (Zhang, Zhao, et al, 2022 ), wheat ( Triticum aestivum L.) (Fang et al, 2020 ), potato (Shang et al, 2022 ), Arabidopsis ( Arabidopsis thaliana [L.] Henyh.) (Zou et al, 2013 ), and other crops.…”

Section: Introductionmentioning

confidence: 99%

Identification of potato Lipid transfer protein gene family and expression verification of drought genes StLTP1 and StLTP7

Wang

Song

Lin³

et al. 2023

Plant Direct

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Lipid transfer proteins (LTPs) are widely distributed in plants and play an important role in the response to stress. Potato (Solanum tuberosum L.) is sensitive to a lack of water, and drought stress is one of the limiting factors for its yield. Therefore, mining candidate functional genes for drought stress and creating new types of potato germplasm for drought resistance is an effective way to solve this problem. There are few reports on the LTP family in potato. In this study, 39 members of the potato LTP family were identified. They were located on seven chromosomes, and the amino acid sequences encoded ranged from 101 to 345 aa. All 39 family members contained introns and had exons that ranged from one to four. Conserved motif analysis of potato LTP transcription factors showed that 34 transcription factors contained Motif 2 and Motif 4, suggesting that they were conserved motifs of potato LTP.Compared with the LTP genes of homologous crops, the potato and tomato (Solanum lycopersicum L.) LTPs were the mostly closely related. The StLTP1 and StLTP7 genes were screened by quantitative reverse transcription PCR combined with potato transcriptome data to study their expression in tissues and the characteristics of their responses to drought stress. The results showed that StLTP1 and StLTP7 were upregulated in the roots, stems, and leaves after PEG 6000 stress. Taken together, our study provides comprehensive information on the potato LTP family that will help to develop a framework for further functional studies.expression analysis, genome-wide identification, LTP, potato) is one of the most important food crops and economic crops in China, and the amounts of its area and yield rank first in the world. Inner Mongolia is one of the primary bases for the production of potato seed and commercial potatoes in China. The annual rainfall is approximately 200-350 mm, and the region is arid and semiarid (du et al., 2013;Zhang, Li, et al., 2022). Drought stress is Dan Wang and Jian Song contributed equally to this work.

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Potato (Solanum tuberosum L.) non-specific lipid transfer protein StLTP6 promotes viral infection by inhibiting virus-induced RNA silencing

Cited by 11 publications

References 58 publications

Systematic and functional analysis of non-specific lipid transfer protein family genes in sugarcane under Xanthomonas albilineans infection and salicylic acid treatment

Systematic and functional analysis of non-specific lipid transfer protein family genes in sugarcane under Xanthomonas albilineans infection and salicylic acid treatment

Tomato chlorosis virus p22 interacts with NbBAG5 to inhibit autophagy and regulate virus infection

Identification of potato Lipid transfer protein gene family and expression verification of drought genes StLTP1 and StLTP7

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