Potato transformation with the HvNHX3 gene and the improvement of transformant salt tolerance

Krivosheeva, A. B.; Varlamova, Tatyana; Yurieva, N. O.; Sobolkova, G. I.; Холодова, В. П.; Belyaev, D. V.

doi:10.1134/s1021443714060119

Cited by 5 publications

(7 citation statements)

References 23 publications

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“…Moreover, K + and Ca 2+ levels were lower in SA than SNA regenerants; however, all the SA regenerants maintained a constant level of these elements both in leaves and roots. Increasing leaf and root internal Na + concentrations have also been reported in tomato [31], sugarcane [25,49], wheat [28,58] and potato [33], as we observed in SNA regenerants. A declining trend was observed in K + , Ca 2+ and Mg 2+ levels in the leaves of SA compared with SNA regenerants.…”

Section: Table 5 In Vitro Performance Assessment Of Adapted and Non-asupporting

confidence: 86%

Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactylifera L.) cultivar Khalas

2020

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Background: Soil salinity causes huge economic losses to agriculture productivity in arid and semiarid areas worldwide. The affected plants face disturbances in osmotic adjustment, nutrient transport, ionic toxicity and reduced photosynthesis. Conventional breeding approaches produce little success in combating various stresses in plants. However, non-conventional approaches, such as in vitro tissue culturing, produce genetic variability in the development of salt-tolerant plants, particularly in woody trees. Results: Embryogenic callus cultures of the date palm cultivar Khalas were subjected to various salt levels ranging from 0 to 300 mM in eight subcultures. The regenerants obtained from the salt-treated cultures were regenerated and evaluated using the same concentration of NaCl with which the calli were treated. All the salt-adapted (SA) regenerants showed improved growth characteristics, physiological performance, ion concentrations and K + /Na + ratios than the salt non-adapted (SNA) regenerants and the control. Regression between the leaf Na + concentration and net photosynthesis revealed an inverse nonlinear correlation in the SNA regenerants. Leaf K + contents and stomatal conductance showed a strong linear relationship in SA regenerants compared with the inverse linear correlation, and a very poor coefficient of determination in SNA regenerants. The genetic fidelity of the selected SA regenerants was also tested using 36 random amplified polymorphic DNA (RAPD) primers, of which 26 produced scorable bands. The primers generated 1-10 bands, with an average of 5.4 bands per RAPD primer; there was no variation between SA regenerants and the negative control. Conclusion: This is the first report of the variants generated from salt-stressed cultures and their potential adaptation to salinity in date palm cv. Khalas. The massive production of salt stress-adapted date palm plants may be much easier using the salt adaptation approach. Such plants can perform better during exposure to salt stress compared to the non-treated date palm plants.

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Section: Table 5 In Vitro Performance Assessment Of Adapted and Non-asupporting

confidence: 86%

Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactylifera L.) cultivar Khalas

2020

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“…This increase in Na + content in plant tissue may be possibly explained by an increase in the expression of genes that encode for V-H + -ATPase and the V-H + -PPase cells [94]. Although not measured in the present work, the increase in these two proton pumps might have energized the Na + (K + )/H + antiport activity at the tonoplast of vacuoles present in potato cells, which promoted the sequestration of high concentration of Na + in vacuoles through the action of NHX transporters [95,96].…”

Section: Impacts Of Soil Salinity On Endogenous Elements Of Potato Plmentioning

confidence: 70%

Synergetic Effects of Zinc, Boron, Silicon, and Zeolite Nanoparticles on Confer Tolerance in Potato Plants Subjected to Salinity

et al. 2019

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Salinity stress is a severe environmental stress that affects plant growth and productivity of potato, a strategic crop moderately sensitive to saline soils. Limited studies are available on the use of combined nano-micronutrients to ameliorate salinity stress in potato plants (Solanum tuberosum L.). Two open field experiments were conducted in salt-affected sandy soil to investigate plant growth, physiology, and yield of potato in response to soil salinity stress under single or combined application of Zn, B, Si, and Zeolite nanoparticles. It was hypothesized that soil application of nanoparticles enhanced plant growth and yield by alleviating the adverse impact of soil salinity. In general, all the nano-treatments applications significantly increased plant height, shoot dry weight, number of stems per plant, leaf relative water content, leaf photosynthetic rate, leaf stomatal conductance, chlorophyll content, and tuber yield, as compared to the untreated control. Furthermore, soil application of these treatments increased the concentration of nutrients (N, P, K, Ca, Zn, and B) in plant tissues, leaf proline, and leaf gibberellic acid hormone (GA3) in addition to contents of protein, carbohydrates, and antioxidant enzymes (polyphenol oxidase (PPO) and peroxidase (POD) in tubers. Compared to other treatments, the combined application of nanoparticles showed the highest plant growth, physiological parameters, endogenous elements (N, P, K, Ca, Zn, and B) and the lowest concentration of leaf abscisic acid (ABA) and transpiration rate. The present findings suggest that soil addition of the aforementioned nanoparticles can be a promising approach to improving crop productivity in salt-affected soils.

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“…The overexpression of the StDREB2 gene in transgenic potato reported playing an important role in salt tolerance, possibly via the regulation of ABA hormone signaling and a mechanism for proline synthesis [165]. In another study, a transgenic potato plant with the IbMYB1 gene produced higher amounts of secondary metabolites containing phenols, flavonoids, and anthocyanins compared with control plants which showed tolerance against salt stress up to 400 mM NaCl [166]. Interestingly, the silencing of the StERF3 gene in potato plants showed higher salt tolerance along with the activation of defense-related genes (PR1, WRKY1, and NPR1) [167].…”

Section: Genetic Engineering Approach To Improve Salt Tolerance In Potatomentioning

confidence: 99%

Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses

Chourasia

Lal

Tiwari

et al. 2021

Life

123

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Among abiotic stresses, salinity is a major global threat to agriculture, causing severe damage to crop production and productivity. Potato (Solanum tuberosum) is regarded as a future food crop by FAO to ensure food security, which is severely affected by salinity. The growth of the potato plant is inhibited under salt stress due to osmotic stress-induced ion toxicity. Salinity-mediated osmotic stress leads to physiological changes in the plant, including nutrient imbalance, impairment in detoxifying reactive oxygen species (ROS), membrane damage, and reduced photosynthetic activities. Several physiological and biochemical phenomena, such as the maintenance of plant water status, transpiration, respiration, water use efficiency, hormonal balance, leaf area, germination, and antioxidants production are adversely affected. The ROS under salinity stress leads to the increased plasma membrane permeability and extravasations of substances, which causes water imbalance and plasmolysis. However, potato plants cope with salinity mediated oxidative stress conditions by enhancing both enzymatic and non-enzymatic antioxidant activities. The osmoprotectants, such as proline, polyols (sorbitol, mannitol, xylitol, lactitol, and maltitol), and quaternary ammonium compound (glycine betaine) are synthesized to overcome the adverse effect of salinity. The salinity response and tolerance include complex and multifaceted mechanisms that are controlled by multiple proteins and their interactions. This review aims to redraw the attention of researchers to explore the current physiological, biochemical and molecular responses and subsequently develop potential mitigation strategies against salt stress in potatoes.

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Potato transformation with the HvNHX3 gene and the improvement of transformant salt tolerance

Cited by 5 publications

References 23 publications

Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactylifera L.) cultivar Khalas

Induced in vitro adaptation for salt tolerance in date palm (Phoenix dactylifera L.) cultivar Khalas

Synergetic Effects of Zinc, Boron, Silicon, and Zeolite Nanoparticles on Confer Tolerance in Potato Plants Subjected to Salinity

Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses

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