Potato virus X displaying the E7 peptide derived from human papillomavirus type 16: a novel position for epitope presentation

Vaculik, Petr; Plchová, Helena; Moravec, Tomáš; Hoffmeisterová, Hana; Čeřovská, Noemi; Šmahel, Michal

doi:10.1007/s11240-014-0634-x

Cited by 7 publications

(4 citation statements)

References 40 publications

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“…In this paper, we extend our previous work on transient expression in plants using plant virus-based vectors (Cerovska et al 2008(Cerovska et al , 2013Vaculik et al 2015). Here, we focus on both transient expression and co-expression of two proteins in BY-2 cells, which had earlier been described in whole plants (Chen et al 2011;Huang et al 2010;Montague et al 2011;Sainsbury et al 2009).…”

Section: Introductionmentioning

confidence: 85%

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

et al. 2020

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Key message This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. Abstract Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.

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Section: Introductionmentioning

confidence: 85%

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

et al. 2020

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“…Vaculik et al (2015a) identified four promising internal transgene insertion positions within the surface loops of the PVX CP. The tested epitope insertion mutant was infectious and produced particles only after amino acid 23 (Vaculik et al, 2015b). However this position still belongs to the N-terminal intrinsically disordered domains of Potexviruses (Solovyev and Makarov, 2016) and is therefore not essential for particle assembly.…”

Section: Master Of Puppets: Strategies For the Creation Of Modified Pmentioning

confidence: 99%

Small, Smaller, Nano: New Applications for Potato Virus X in Nanotechnology

2019

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Nanotechnology is an expanding interdisciplinary field concerning the development and application of nanostructured materials derived from inorganic compounds or organic polymers and peptides. Among these latter materials, proteinaceous plant virus nanoparticles have emerged as a key platform for the introduction of tailored functionalities by genetic engineering and conjugation chemistry. Tobacco mosaic virus and Cowpea mosaic virus have already been developed for bioimaging, vaccination and electronics applications, but the flexible and filamentous Potato virus X (PVX) has received comparatively little attention. The filamentous structure of PVX particles allows them to carry large payloads, which are advantageous for applications such as biomedical imaging in which multi-functional scaffolds with a high aspect ratio are required. In this context, PVX achieves superior tumor homing and retention properties compared to spherical nanoparticles. Because PVX is a protein-based nanoparticle, its unique functional properties are combined with enhanced biocompatibility, making it much more suitable for biomedical applications than synthetic nanomaterials. Moreover, PVX nanoparticles have very low toxicity in vivo , and superior pharmacokinetic profiles. This review focuses on the production of PVX nanoparticles engineered using chemical and/or biological techniques, and describes current and future opportunities and challenges for the application of PVX nanoparticles in medicine, diagnostics, materials science, and biocatalysis.

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“…Studies performed by fusing peptides (variable in length and amino acid composition) to a N-terminally truncated CP (a natural PVX mutant lacking the first 21 amino acids) have indicated that also isoelectric point, Ser/Thr and Trp content in the heterologous sequence may affect viral fitness ( Lico et al, 2006 ). Recently, the insertion of a heterologous sequence between amino acids 23 and 24 at the N-terminus of the CP has also been reported ( Vaculik et al, 2015 ). Nonetheless, up to now no CP domains other than the N-terminus have been identified that can harbor fusion peptides/proteins.…”

Section: Pvx Nanoparticles For Subunit Vaccine Deliverymentioning

confidence: 99%

The Two-Faced Potato Virus X: From Plant Pathogen to Smart Nanoparticle

2015

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Potato virus X (PVX) is a single-stranded RNA plant virus, historically investigated in light of the detrimental effects on potato, the world’s fourth most important food commodity. The study of the interactions with cells, and more generally with the plant, both locally and systemically, significantly contributed to unveil the mechanisms underlying gene silencing, fundamental not only in plant virology but also in the study of gene expression regulation. Unraveling the molecular events of PVX infection paved the way for the development of different viral expression vectors and consequential applications in functional genomics and in the biosynthesis of heterologous proteins in plants. Apart from that, the ease of manipulation and the knowledge of the virus structure (particle dimensions, shape and physicochemical features) are inspiring novel applications, mainly focused on nanobiotechnology. This review will lead the reader in this area, spanning from fundamental to applied research, embracing fields from plant pathology to vaccine and drug-targeted delivery, imaging and material sciences. Due to the versatile moods, PVX holds promise to become an interesting nanomaterial, in view to create the widest possible arsenal of new “bio-inspired” devices to face evolving issues in biomedicine and beyond.

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Potato virus X displaying the E7 peptide derived from human papillomavirus type 16: a novel position for epitope presentation

Cited by 7 publications

References 40 publications

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

Small, Smaller, Nano: New Applications for Potato Virus X in Nanotechnology

The Two-Faced Potato Virus X: From Plant Pathogen to Smart Nanoparticle

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