Potencial antimicrobiano de resíduos agroindustriais sobre Listeria monocytogenes

Abstract

Antimicrobial potential of agroindustrial residues on Listeria monocytogenesThe phenolic compounds and glucosinolates have been investigated for their antimicrobial activity against several pathogenic microorganisms, including Listeria monocytogenes, a dangerous contaminant of food. This study has as main objective valuate agroindustrial residues with activity against Listeria monocytogenes, well as evaluate the chemical composition, aimed at their application in food industry. In this work we analyzed 23 agroindustrial residues. The extracts of these residues were prepared from five solvents (hexane, chloroform, ethyl acetate, ethanol:water (80:20 v/v) and water), which were used in evaluating the antimicrobial potential against Listeria monocytogenes by the agar diffusion test. The extracts that tested positive were selected for analysis of Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), flow cytometry and chemical composition by GC-MS. L. monocytogenes showed sensibility only to aqueous extracts of stalk of broccoli and peel pumpkin and chloroform extract of papaya seed showing MIC values of 102.4 mg / mL,> 102.4 mg / mL and 6.4 mg / mL, respectively. The flow cytometry analysis showed changes in cell morphology, front of to tested extracts. The main action of the extracts was as a function the inhibition of growth of microorganisms and reducing its population. The extracts showed a peculiar chemical composition. In extracts of broccoli stalks and peel pumpkin were found some phenolic acids and organic, while the extract in papaya seed found the single compound with antimicrobial potential was benzyl-isothiocyanate. Given the difficulties in preventing food contamination by L. monocytogenes, the use of natural antimicrobial compounds derived from agroindustrial residues shows promise and can be an alternative to aid in safety of food.

Avaliação da atividade antimicrobiana de resíduos agroindustriais sobre patógenos de doenças orais e sistêmicas

“…Esses resíduos exibem, além da atividade antioxidante e antimicrobiana (antibacterianas, antifúngicas, antiprotozoários), também apresentam ações antivirais e antiparasitárias contra patógenos orais e sistêmicos (Seligman, 2004;Cavalcanti et al, 2008;Corrêa, 2011;Jeon et al, 2011;Camargo, 2014) e, ainda, propriedades antialérgicas, antiaterogênicas, anti-inflamatórias, antitrombóticas e cardioprotetoras, dentre outras (Balasundram et al, 2006;Dimitrios, 2006).…”