Potencial germinativo e morfoanatomia foliar de plântulas de pinhão-manso originadas de germoplasma criopreservado

Silva, Rafael de Carvalho; Camillo, Julcéia; Luis, Zanderluce Gomes; Scherwinski‐Pereira, Jonny Everson

doi:10.1590/s0100-204x2011000800008

Cited by 8 publications

(10 citation statements)

References 21 publications

(23 reference statements)

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“…seeds, the humidity degree of 12.5 at 4.2% assured an adequate physiological performance of the seeds under liquid nitrogen. For orthodox seeds, contents with humidity below 10% are recommended; however, the favorable humidity interval for freezing differs according to the species (Silva et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

“…On this regard, some authors state that after the cryopreservation, abnormal germination may occur, or even death due to internal injuries, which may be related to the characteristics of certain seeds, such as size, water content and chemical composition (Goldfarb et al, 2010;Silva et al, 2011). However, sucrose was promising regarding the reduction of the injury caused to the seeds by the ultra-low temperatures, maintaining the emergency percentage of seedlings on the same levels than the control treatment.…”

Section: Resultsmentioning

confidence: 99%

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Seed cryopreservation of Passiflora species

et al. 2016

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-The aim of this study was to evaluate the cryopreservation of seeds of Passiflora species, using different cryoprotectants. The completely randomized design was used with four treatments (T1 -DMSO at 7%; T2 -0.3 M sucrose; T3 -storage in NL 2 without cryoprotectants; T4 -control) and five replications of 50 seeds. The seeds were stored for 120 hours in NL 2 (-196 °C), and thawing was conducted in a water bath (37 °C) for 20 minutes, proceeding with the germination and vigor tests. The data were submitted to ANOVA and Tukey's test (5% of probability) using the SISVAR program. In P. mucronata, P. suberosa and P. edulis seeds, the responses obtained were favorable to cryopreservation without the use of cryoprotectants in regards to P. micropetala, there was a considerable loss of germination potential after storage in NL 2 . Given these circunstances, cryopreservation can be used for long-term conservation of Passiflora mucronata, P. suberous and P. edulis, without the use of cryoprotectants.Index terms: passion fruit, storage, germination.Criopreservação de sementes de espécies de Passiflora RESUMO: Objetivou-se avaliar a criopreservação de sementes de espécies de Passiflora, utilizando-se diferentes crioprotetores. Foi utilizado o Delineamento Inteiramente Casualizado, com quatro tratamentos (T 1 -DMSO a 7%; T 2 -sacarose a 0,3 M; T 3 -armazenamento em NL 2 sem crioprotetor; T 4 -controle) e cinco repetições de 50 sementes. As sementes foram armazenadas durante 120 horas em NL 2 (-196 °C), realizou-se o descongelamento em banho-maria (37 °C) durante 20 minutos, e prosseguiram-se os testes de germinação e vigor. Os dados foram submetidos à análise de variância e teste de Tukey (5% probabilidade) pelo programa SISVAR. Em sementes de P. mucronata, P. suberosa e P. edulis foram obtidas respostas favoráveis à criopreservação sem o uso de crioprotetores, e em P. micropetala houve perda considerável do potencial germinativo após armazenamento em NL 2 . Diante disso, a criopreservação pode ser utilizada para conservação em longo prazo de sementes de Passiflora mucronata, P. suberosa e P. edulis, sem o uso de crioprotetores.Termos para indexação: maracujá, armazenamento, germinação.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Seed cryopreservation of Passiflora species

et al. 2016

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“…De acordo com SANTOS (2013), a desidratação pode provocar modificações deletérias, afetando o metabolismo celular. Há um intervalo de umidade aceitável a ser atingido pelos explantes antes do congelamento, porém, a remoção da umidade abaixo de limite tolerável afeta diretamente a estrutura molecular e a integridade das organelas (SILVA et al, 2011;SERSHEN et al, 2012), o que se reflete em menor regeneração.…”

Section: Resultsunclassified

“…A desidratação pode ser obtida pela evaporação da água, promovida pela exposição do material à sílica gel ou câmara de fluxo laminar ou pelo uso de crioprotetores que provocam a desidratação osmótica do material (SANTOS, 2004). A desidratação pode provocar modificações deletérias afetando o metabolismo celular, havendo um intervalo de umidade aceitável a ser atingido pelos explantes antes do congelamento, porém, a remoção da umidade abaixo de limite tolerável afeta diretamente a estrutura molecular e a integridade das organelas (SILVA et al, 2011;SERSHEN et al, 2012), o que se reflete em menor regeneração após a etapa do descongelamento.…”

Section: Introductionunclassified

Encapsulamento, crioproteção e desidratação na capacidade regenerativa de ápices caulinares de Genipa americana

et al. 2015

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“…Accordingly, Astronium urundeuva (urunday) seeds maintained their viability when cryopreserved with 6% m c water content (Medeiros et al, 1992). Similarly, Jatropha curcas L. (Barbados nut) seeds maintained their vigour index when stored in liquid nitrogen or in vapour (Goldfarb et al, 2010) and their high viability when cryopreserved with 8% m c water content (Silva et al, 2011). Tabebuia chrysotricha (golden trumpet tree) seeds showed higher germination when cryopreserved with 4% m c water content (Tresena et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation as an alternative for conservation of Anacardium humile achene (Monkey nut)

Barbosa¹,

Sales²,

Silva³

et al. 2019

Aust J Crop Sci

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The present study aimed to evaluate the possibility of using cryopreservation method for conservation of cajuzinho-do-cerrado achene (Anacardium humile). To accomplish this aim, the physiological quality of cryopreserved achenes was compared with the quality of samples stored under other conditions. The quality of cryopreserved achenes with different water contents was also evaluated to establish a water-content limit for seed-viability maintenance. Achenes with physiological maturity were stored in liquid nitrogen at -196 °C with the following water contents: 28 (initial content) 14, 12, 8 and 5% moisture content (m c ). Subsequently, the achenes were subjected to 3 thawing methods: slow/gradual, fast and microwave thawing. Achenes were also subjected to different six conservation methods: cryopreservation in liquid nitrogen (LN) at -196 °C, cryopreservation using 10% cryoprotectants (Dimethyl sulfoxide (DMSO) or Glycerol), 10 °C biochemical oxygen demand (BOD) incubator, -80 °C ultra-low freezer and room temperature (25 °C ±3), with testing after storage in these environments for 5 and 10 months. The treatment effect on achene, physiological quality was assessed by seed germination (percentage) and vigour (germination speed index, electrical conductivity and root and shoot length). The results showed that reduction in the water content considerably reduced the germinating potential of Anacardium humile achenes for cryopreservation. Conversely, the thawing methods had no effect on the physiological quality of cryopreserved achenes; thereby, allowing the choice of the most practical thawing method (microwave). We also concluded that cryopreservation in liquid nitrogen may be used as a viable alternative for conservation of Anacardium humile achene.

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Potencial germinativo e morfoanatomia foliar de plântulas de pinhão-manso originadas de germoplasma criopreservado

Cited by 8 publications

References 21 publications

Seed cryopreservation of Passiflora species

Seed cryopreservation of Passiflora species

Encapsulamento, crioproteção e desidratação na capacidade regenerativa de ápices caulinares de Genipa americana

Cryopreservation as an alternative for conservation of Anacardium humile achene (Monkey nut)

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