Potency of Nucleopolyhedrovirus Genotypes for European and Asian Gypsy Moth (Lepidoptera: Lymantriidae)

Podgwaite, J. D.; Martemyanov, V. V.; Slavicek, James M.; Bakhvalov, S. A.; Pavlushin, Sergey V.; Hayes-Plazolles, Nancy; Zerillo, R. T.

doi:10.18474/0749-8004-48.4.332

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…In contrast, Podgwaite and coworkers (Bakhvalov et al, 2005;Podgwaite et al, 2006) found little difference between the activities of Gypchek and Virin ENSh against Western Siberian strains of gypsy moth. The same group also reported that an LdMNPV isolate from a Western Siberian host population killed Western Siberian gypsy moth larvae with LC 50 values that were equal to or lower than LC 50 s obtained with plaque isolates derived from Gypchek (Podgwaite et al, 2013).…”

Section: Discussionmentioning

confidence: 89%

Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strains

Harrison

Rowley

Keena

2016

Journal of Invertebrate Pathology

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Isolates of the baculovirus species Lymantria dispar multiple nucleopolyhedrovirus have been formulated and applied to suppress outbreaks of the gypsy moth, L. dispar. To evaluate the genetic diversity in this species at the genomic level, the genomes of three isolates from Massachusetts, USA (LdMNPV-Ab-a624), Spain (LdMNPV-3054), and Japan (LdMNPV-3041) were sequenced and compared with four previously determined LdMNPV genome sequences. The LdMNPV genome sequences were collinear and contained the same homologous repeats (hrs) and clusters of baculovirus repeat orf (bro) gene family members in the same relative positions in their genomes, although sequence identities in these regions were low. Of 146 non-bro ORFs annotated in the genome of the representative isolate LdMNPV 5-6, 135 ORFs were found in every other LdMNPV genome, including the 37 core genes of Baculoviridae and other genes conserved in genus Alphabaculovirus. Phylogenetic inference with an alignment of the core gene nucleotide sequences grouped isolates 3041 (Japan) and 2161 (Korea) separately from a cluster containing isolates from Europe, North America, and Russia. To examine phenotypic diversity, bioassays were carried out with a selection of isolates against neonate larvae from three European gypsy moth (Lymantria dispar dispar) and three Asian gypsy moth (Lymantria dispar asiatica and Lymantria dispar japonica) colonies. LdMNPV isolates 2161 (Korea), 3029 (Russia), and 3041 (Japan) exhibited a greater degree of pathogenicity against all L. dispar strains than LdMNPV from a sample of Gypchek. This study provides additional information on the genetic diversity of LdMNPV isolates and their activity against the Asian gypsy moth, a potential invasive pest of North American trees and forests.

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Section: Discussionmentioning

confidence: 89%

Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strains

Harrison

Rowley

Keena

2016

Journal of Invertebrate Pathology

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“…Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) is a highly pathogenic, host-specific Alphabaculovirus that has been used as the basis of biological insecticides for the control of the gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) [ 28 , 29 ]. To date, many different geographic strains of LdMNPV have been genetically characterized [ 30 , 31 , 32 ] and biological activity against gypsy moth larvae has been quantified [ 10 , 33 , 34 , 35 , 36 ]. It has been found that strains may differ significantly in terms of virulence.…”

Section: Introductionmentioning

confidence: 99%

A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in Lymantria Dispar L.

Akhanaev

Belousova

Lebedeva

et al. 2020

Insects

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Baculoviruses can persist in insect host organisms after infection and may be vertically transmitted to the next generation, in which they may be reactivated. The goal of the present study was to compare the efficiency of the vertical transmission of high- and low-virulence strains and the subsequent reactivation of Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) in the offspring of Lymantria dispar L. adults who survived after viral infection. As a result of parental infection, the fecundity of survived females, pupae weight, and fertility were significantly different compared to the untreated insects. However, differences in these parameters between high- and low-virulence strains were not observed. The prevalence of virus strains in the offspring measured by quantitative polymerase chain reaction also did not differ. When the larvae reached the fourth instar, they were starved to activate the vertically transmitted virus. The frequency of virus activation in the experiment was not dependent on the virulence of the virus strains. These results are helpful for understanding the strategy of virus survival in nature and for the selection of the most effective strains with transgenerational effects in the years following pest treatment.

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“…Here we compare genomes of closely related genetic variants of Lymantria dispar multiple NPV (LdMNPV) that differ in virulence. Methods and Algorithms: Five viral genotypes, differing in virulence, were isolated from the standard strain of NPV in the biopesticide "Gypchek" [2]. Complete genomes of these five genotypes were sequenced using Illumina technology by paired end sequencing of fragment genomic libraries, and assembled by SPAdes 3.9.0 [3].…”

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confidence: 99%

Comparative genome analysis of related Lymantria dispar nucleopolyhedrovirus isolates differing in virulence

2018

Импозиум «Биоразнообразие: Геномика И Эволюция»

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Motivation and Aim: Nucleopolyhedroviruses (NPVs, Baculoviridae) are specific viruses of insects that are used for pest control. High variation in virus virulence depends upon genetic factors [1]. Here we compare genomes of closely related genetic variants of Lymantria dispar multiple NPV (LdMNPV) that differ in virulence. Methods and Algorithms: Five viral genotypes, differing in virulence, were isolated from the standard strain of NPV in the biopesticide "Gypchek" [2]. Complete genomes of these five genotypes were sequenced using Illumina technology by paired end sequencing of fragment genomic libraries, and assembled by SPAdes 3.9.0 [3]. Initial assemblies consisted of 1-3 scaffolds with a total assembly length of 159-174 kbp. Genome coverage was in range of 200x-850x. Closing of scaffold gaps was performed by Sanger sequencing of amplicons. To determine genetic relatedness among studied LdMNPV variants a phylogenetic tree was reconstructed based on a set of core loci. Comparative analysis of candidate genes was performed to reveal genetic determinates of variation in virulence. Results: Phylogenetic analyses indicated recent divergence of LdMNPV-studied isolates from a common ancestor. We found a number of nonsynonymous nucleotide substitutions and indels in many genes. However, we could not find a locus that could be considered as the main effector of observed variation in virulence. This fact indicated a complex genetic nature of virulence variation among closely related virus isolates. Conclusion: Variation in virulence among related LdMNPV isolates can be explained by the complex effect of different loci. Acknowledgements: the study was supported by . References

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Potency of Nucleopolyhedrovirus Genotypes for European and Asian Gypsy Moth (Lepidoptera: Lymantriidae)

Cited by 5 publications

References 13 publications

Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strains

Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strains

A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in Lymantria Dispar L.

Comparative genome analysis of related Lymantria dispar nucleopolyhedrovirus isolates differing in virulence

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