Potency of Radical Scavenging Activity and Determination of Total Phenolic Content of Five Ethanolic Extract of Rhizome Zingiberaceae Family

Abstract

Several studies show that some plants and fruits could protect human body from free radical danger exposure. Zingiberaceae family has some chemical substances that have antiradical activity, such as phenolic compound and flavonoid. The aim of this study is to determine the correlation of total phenolic compound towards its antiradical activity. Antiradical activity assay was determined by DPPH method (1,1 difenil-2-pikrilhidrazil). The total phenolic compound is determined by spectrofotometry using Folin-Ciocalteu reagent and counted as GAE (Gallic Acid Equivalent). Based on this study, the total phenolic concentration of ethanolic extract of jahe (Zingiber officinale (L.) Rosc), kencur (Kaemferia galanga L.), lengkuas (Languas galanga), lempuyang gajah (Zingiber littorale) and lempuyang pahit (Zingiber americana) is 73.99 mg/g; 23.848 mg/g; 37.841 mg/g; 49.725 mg/g and 40.802 mg/g respectively. The radical scavenging activity (IC50) of jahe (27.38 µg/mL), lengkuas (93,27 µg/mL), lempuyang pahit (105,08 µg/mL), meanwhile lempuyang gajah and kencur show low activity of radical scavenging.

Prophylactic effects of Kaempferia Galanga against Plasmodium berghei: in vivo study

“…Kaempferia galanga contains phenolic compounds and flavonoids, understood as their antioxidant properties. 22,14,23 The phenolic compound of K. galanga possesses moderate and high antioxidant activity. 24,14 Typically, as antioxidants, flavonoids may reduce oxidative stress or increase the antioxidant capacity, 23 , and radical scavenging.…”

“…22,14,23 The phenolic compound of K. galanga possesses moderate and high antioxidant activity. 24,14 Typically, as antioxidants, flavonoids may reduce oxidative stress or increase the antioxidant capacity, 23 , and radical scavenging. 24 Although the total phenolic level and radical scavenging activity were not as high as other Zingiberaceae family.…”

“…24 Although the total phenolic level and radical scavenging activity were not as high as other Zingiberaceae family. 23 Phenolic compounds have also been widely acknowledged as an antimalarial. 25 Antimalarial activity of phenol compounds is accurately inhibiting heme polymerization.…”