POTENSI AKTIVITAS ANTIOKSIDAN METABOLIT SEKUNDER DARI BAKTERI ENDOFIT PADA DAUN Moringa oleifera L

Abstract

[POTENTIAL ANTIOXIDANT ACTIVITY OF SECONDARY METABOLITES FROM ENDOPHYTE BACTERIA ON Moringa oleifera L LEAF] This research aims to know the similarities between secondary compound metabolites produced by endophyte bacterial on host leaves and from Moringa oleifera L (kelor) leaves as well as determine the magnitude of antioxidant activity compounds of secondary metabolites produced by bacterial endophyte. Based on the results of the test compound is secondary metabolite in both samples, for secondary metabolite compounds in the extract of the leaves of M. oleifera L. contains identified steroid compounds, flavonoids, saponins, tannins and phenolic compounds, while for metabolites secondary bacterial endophyte undetectable on the existence of any secondary metabolite compounds.For testing of antioxidants on endophyte bacterial extracts of leaves of M. oleifera L is done using the DPPH method, performed on variations of the concentration of 200, 400, 600, 800, and 1000 ppm with Ascorbic acid as a comparison. The results of the test get an IC50 of M. oleifera L leaf extract at 243.67 ppm, and supernatan extract from endophyte bacterial at 492 ppm. The IC50 values showed antioxidant activity in secondary metabolite compounds derived from extracts of the leaves of M. oleifera L which has an extreme antioxidant category. The amount of antioxidant activity for secondary metabolite compound extracts of bacterial endophyte on leaves of M. oleifera L category is feeble, and to extract the supernatan activity is weak compared to the value of the antioxidant activity of Ascorbic acid.

Moringa oleifera fruit extract as a potential antioxidant against liver injury by 2-Nitropropane induction in obese male mice model: pre-clinical study

“…[2][3][4] M. oleifera fruits extract contains flavonoids as antioxidants. 2,35 The antioxidant activity of flavonoids comes from their ability to donate hydrogen atoms and the ability to chelate metal. 18 M. oleifera fruits extract was also anti-obesity because it can down-regulate leptin and resistin mRNA expression while at the same time up-regulating the expression of adiponectin mRNA.…”

Moringa oleifera fruit extract as a potential antioxidant against liver injury by 2-Nitropropane induction in obese male mice model: pre-clinical study

“…M. oleifera fruits extract contains flavonoids as antioxidants. 2,35 The antioxidant activity of flavonoids comes from their ability to donate hydrogen atoms and the ability to chelate metal. 18 M. oleifera fruits extract was also anti-obesity because it can down-regulate leptin and resistin mRNA expression while at the same time up-regulating the expression of adiponectin mRNA.…”

OPTIMASI FORMULA SEDIAAN GRANUL EFFERVESCENT EKSTRAK DAUN KELOR (Moringa oleifera L.)

“…Berdasarkan hasil uji fitokimia terhadap daun (Moringa oleifera L.) diketahui mengandung berbagai senyawa metabolit sekunder seperti tanin, steroid, triterpenoid, flavonoid, saponin, antrakuinon, dan alkaloid. Hasil berbagai penelitian menunjukkan bahwa senyawa-senyawa tersebut mampu bertindak sebagai antioksidan dan memiliki potensi sebagai obat (Yati et al, 2018).…”