POTENSI ISOLAT Bacillus sp. ENG-4 YANG BERASOSIASI DENGAN SPONS Aplysina sp. PENGHASIL SENYAWA ANTIMIKROB ASAL PULAU ENGGANO

Wibowo, Risky Hadi; Sipriyadi, Sipriyadi; Darwis, Welly; Kamilah, Santi Nurul; Pertiwi, Hizkia Puspa; Pertiwi, Reza

doi:10.31186/jenggano.5.1.1-10

Cited by 22 publications

(5 citation statements)

References 4 publications

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“…The results from previous screening showed that 16 isolates of bacteria were isolated [14]. Four bacterial isolates could produced antifungal compounds.…”

Section: Screening Of Bacteria Producing Anti-fungalmentioning

confidence: 99%

“…collected from Enggano Island against Candida albicans. Based on research conducted by [14] that Aplysina sp. has antibacterial and antifungal compounds tested against pathogenic bacteria and yeast.…”

Section: Introductionmentioning

confidence: 99%

“…Sponge is a group of multicellular animals, has characteristic porous body, and invertebrate organisms are useful in producing bioactive compounds. Bacteria associated with sponges can be an alternative source of new bioactive compounds, especially antimicrobial [14].…”

Section: Introductionmentioning

confidence: 99%

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Antifungal Activity of Bacteria Associated Aplysina sp. Sponge Collected from Enggano Island, North Bengkulu, Indonesia Against Candida albicans

Wibowo¹,

Sipriyadi²,

Darwis³

et al. 2021

Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 202

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Sponges are the subject of interesting antibiotic development studies because the sponges form associations with various microbes and are rich in bioactive compounds. Bacteria associated with sponges are able to produce bioactive compounds, which have the potential to be antimicrobial such as, antibacterial, antifungal and antiviral. Antifungal compounds are bioactive compounds that have the ability to inhibit the growth of pathogenic fungi of Candida albicans. This fungal is an opportunistic pathogen fungi that can cause candidiasis. This study aimed to examine the antifungal activity of bacteria associated Aplysina sp. sponge from Enggano Island, North Bengkulu, Indonesia against Candida albicans. Antagonistic tests were carried out in three stages, by using isolates, pellets and supernatants respectively. The antagonistic assay results showed that four isolates were able to inhibit the growth of the fungus Candida albicans in Vitro, i.e APD3, APD10, APD11 and APD15. The highest inhibitory activity was resulted by APD10 isolate with a clear zone area of 14.9 mm in culture, 14,0 mm in pellets and 15.1 mm in supernatants. The morphological, Gram staining and biochemical characterization showed that the four isolates had a close relationship with the genus of Bacillus.

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“…The results from previous screening showed that 16 isolates of bacteria were isolated [14]. Four bacterial isolates could produced antifungal compounds.…”

Section: Screening Of Bacteria Producing Anti-fungalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antifungal Activity of Bacteria Associated Aplysina sp. Sponge Collected from Enggano Island, North Bengkulu, Indonesia Against Candida albicans

Wibowo¹,

Sipriyadi²,

Darwis³

et al. 2021

Proceedings of the International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 202

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“…Aplysina sp. sponge samples were collected previous research have been done from the coastal waters of Dua Island, Enggano Island located in North Bengkulu Regency (Wibowo et al, 2020). The samples obtained were immediately carried out in the preparation.…”

Section: Aplysina Sp Sponges Collectionmentioning

confidence: 99%

Antibacterial Activities of Pseudomonas orientalis APD 16 Isolate Sponge-Associated Aplysina sp. from Enggano Island Against Escherichia coli and Staphylococcus aureus

Wibowo¹

2020

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Sponge is one of the invertebrates from the Porifera phylum. Sponge bodies have structural complexity with different cell layers. The sponge has many pores (ostium) on the surface of their body as a filter feeder. Sponge is recognized as organisms that have the potential because they can produce metabolites. Secondary metabolites produced by sponges are the result of the association of sponges with bacteria. The sponge used in this study is the Aplysina sp. sponge collected from Enggano island, Bengkulu Province. Aplysina sp. sponge is known to contain metabolites with antibacterial, antifungal and cytotoxic activity on cancer cells. This study aims to identify of potential isolate associated with Aplysina sp. sponge collected from Enggano island. Isolation of bacteria from Aplysina sp. sponge using Sea Water Complete (SWC) media. The isolate was screened by antagonistic test, morphological characters, Gram-staining, biochemical test and molecular identification. Based on the antagonistic test, APD 16 isolate could inhibit Escherichia coli and Staphylococcus aureus in Vitro. APD 16 isolate was identified molecularly using of 16S rRNAgenes analysis and it genetically close with Pseudomonas orientalis.

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“…Berdasarkan pernyataan tersebut bakteri yang bersimbiosis dengan Ptilocaulis marquezii dapat dijadikan sebagai sumber alternatif baru sebagai antibakteri dan juga ekstrak yang berasal dari Ptilocaulis marquezii seperti Aplysina sp. yang memiliki bakteri simbion berupa bacillus dan memiliki aktivitas antibakteri E.coli dan S. aureus (Wibowo et al, 2020).…”

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Uji Ekstrak Spons Laut Jenis Ptilocaulis marquezii dari Perairan Kendit sebagai Potensi Antibakteri Escherichia coli

et al. 2023

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Guna mengetahui potensi bioaktivitas senyawa yang terkandung serta mengetahui uji ekstrak spons Ptilocaulis marquezii terhadap pertumbuhan bakteri Eschercia coli. Penelitian dilakukan secara eksperimen berupa uji ekstrak berupa pembuatan ekstrak spons Ptilocaulis marquezii dengan maserasi serta evaporasi dengan rotary vacuum evaporator pada suhu 40°C hingga ekstrak terbentuk. Dilakukan pengujian dengan metode Difusi cakram atau Kirby-Bauer test. Pengujian fitokimia guna mengetahui kandungan senyawa aktif. Hasil yang diperoleh didapatkan ekstrak dengan konsentrasi 40% yang memiliki kemampuan sedang. Pada uji fitokimia mengandung alkoloid dan triptenoid. Ekstrak spons Ptilocaulis marquezii pada konsentrasi 40% dengan zona hambat sebesar 9.5 mm dalam kategori sedang dan pada konsentrasi lainnya yaitu 10%, 20%, 60% dan 80% berkategori lemah. Senyawa alkaloid menghambat pertumbuhan Escherichia Coli dengan merusak susunan peptidoglycan. Pada senyawa triterpenoid merusak membran plasma pada bakteri Escherichia coli. Knowing the potential bioactivity of the compounds contained and knowing the test of the extract of the Ptilocaulis marquezii sponge of Eschercia coli bacteria. The research was carried out experimentally in the form of extract testing in the form of making Ptilocaulis marquezii sponge extract by maceration and evaporation with a rotary vacuum evaporator at a temperature of 40°C until the extract was formed. The test was carried out using the disc diffusion method or the Kirby-Bauer test. Phytochemical testing to determine the content of active compounds. The results obtained were extracts with a concentration of 40% which had moderate abilities. The phytochemical test contains alkaloids and triptenoids. Ptilocaulis marquezii sponge extract at a concentration of 40% with an inhibition zone of 9.5 mm in the medium category and at other concentrations of 10%, 20%, 60% and 80% in the weak category. Alkaloids inhibit the growth of Escherichia Coli by destroying the peptidoglycan structure. The triterpenoid compounds damage the plasma membrane of Escherichia coli bacteria.

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POTENSI ISOLAT Bacillus sp. ENG-4 YANG BERASOSIASI DENGAN SPONS Aplysina sp. PENGHASIL SENYAWA ANTIMIKROB ASAL PULAU ENGGANO

Cited by 22 publications

References 4 publications

Antifungal Activity of Bacteria Associated Aplysina sp. Sponge Collected from Enggano Island, North Bengkulu, Indonesia Against Candida albicans

Antifungal Activity of Bacteria Associated Aplysina sp. Sponge Collected from Enggano Island, North Bengkulu, Indonesia Against Candida albicans

Antibacterial Activities of Pseudomonas orientalis APD 16 Isolate Sponge-Associated Aplysina sp. from Enggano Island Against Escherichia coli and Staphylococcus aureus

Uji Ekstrak Spons Laut Jenis Ptilocaulis marquezii dari Perairan Kendit sebagai Potensi Antibakteri Escherichia coli

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