Potent Cytotoxic Lignans from Justicia procumbens and Their Effects on Nitric Oxide and Tumor Necrosis Factor-α Production in Mouse Macrophages

Day, Shiow‐Hwa; Lin, Ying-Zu; Tsai, Max; Tsao, Lo‐Ti; Ko, Horng‐Huey; Chung, Mei‐Ing; Lee, Jeng-Chang; Wang, Jih-Pyang; Won, Shen‐Jeu; Lin, Chun‐Nan

doi:10.1021/np0101651

Cited by 91 publications

(75 citation statements)

References 16 publications

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“…Justicidin A, an arylnaphthalide lignan isolated from the acanthaceous plant Justicia procumbens (Fukamiya and Lee, 1986), has been shown to have strong antiviral activity (Asano et al, 1996), potent cytotoxic effects (Day et al, 1999(Day et al, , 2002, and enhanced TNF-␣ generation in LPS-stimulated RAW 264.7 cells at lower concentrations (Յ1 M justicidin A) (Day et al, 2002). Surprisingly, we found that at concentrations greater than 5 M, justicidin A significantly inhibited LPS-stimulated TNF-␣ secretion in the present study.…”

contrasting

confidence: 80%

Justicidin A Inhibits the Transport of Tumor Necrosis Factor-α to Cell Surface in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

Tsao¹,

Lin²,

Wang³

2004

Mol Pharmacol

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Exposure of macrophages to lipopolysaccharide (LPS) induces release of tumor necrosis factor-␣ (TNF-␣), which is initially synthesized as a 26-kDa pro-TNF-␣ followed by proteolytic processing to a 17-kDa secreted form. In this study, justicidin A, an arylnaphthalide lignan isolated from Justicia procumbens, was found to inhibit LPS-stimulated TNF-␣ release from RAW 264.7 macrophages in a concentration-and time-dependent manner, and the underlying mechanism was investigated. In the presence of justicidin A, challenge with LPS increased the steady-state level of the 26-kDa membrane-bound form of TNF-␣ protein, whereas justicidin A had little effect on the expression of TNF-␣ mRNA and on the synthesis of pro-TNF-␣ protein. Results of the pulse-chase experiment, revealed that the conversion of pro-TNF-␣ to mature TNF-␣ was inhibited by justicidin A. Moreover, justicidin A suppressed the transport of TNF-␣ to cell surface as analyzed by flow cytometry. The immunofluorescence analysis demonstrated that large amounts of LPS-induced TNF-␣ accumulated primarily within Golgi complex. These results indicate that justicidin A inhibits TNF-␣ release at the step of transport of pro-TNF-␣ to cell surface, and this leads to the accumulation of TNF-␣ in Golgi complex in RAW 264.7 macrophages.

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contrasting

confidence: 80%

Justicidin A Inhibits the Transport of Tumor Necrosis Factor-α to Cell Surface in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

Tsao¹,

Lin²,

Wang³

2004

Mol Pharmacol

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“…Justicidin A was isolated from J. procumbens plants [9]. DiOC 6 (3) was obtained from Molecular Probes (Eugene, OR).…”

Section: Methodsmentioning

confidence: 99%

“…Justicia procumbens (J. procumbens) is a traditional herbal remedy in Taiwan for fever, pain, and cancer [6,7]. Justicidin A, purified from a methanolic extract of J. procumbens, has various biological activities including suppression of tumor cell growth [8,9] and release of TNF-a [10]. The purpose of present study was to determine whether apoptosis is stimulated by justicidin A-treatment of human HCC.…”

Section: Introductionmentioning

confidence: 99%

Caspase‐8 acts as a key upstream executor of mitochondria during justicidin A‐induced apoptosis in human hepatoma cells

Huang

et al. 2006

FEBS Letters

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Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Dw m ), and caused the release of cytochrome c and Smac/DIABLO in Hep 3B and Hep G2 cells. Justicidin A also reduced Bcl-x L and increased Bax and Bak in mitochondria. Caspase-8 inhibitor (Z-IETD) attenuated the justicidin A-induced disruption of Dw m . Growth of Hep 3B implanted in NOD-SCID mice was suppressed significantly by oral justicidin A (20 mg/kg/day). These results indicate that justicidin A-induced apoptosis in these cells proceeds via caspase-8 and is followed by mitochondrial disruption. Supplementary materials are available at http://myweb. ncku.edu.tw/~a725/.

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“…Lignans 30, 31, and 37, isolated from J. ciliata, showed significant citotoxic effects toward a number of cancer cell types (human hepatomacellular carcinoma, human cervical carcinoma, human colorectal adenocarcinoma, human colorectal carcinoma, and human breast cancer) (Day et al, 2002). Lignan 31 also displayed potent cytotoxic effects against T-24, CaSki, SiHa, HT-3, PLC/PRF/5, and 212 cells in vitro (Day et al, 1999).…”

Section: Biological Activity Of Compounds Isolated From Species Of Jumentioning

confidence: 98%

Chemical constituents and biological activities of species of Justicia: a review

Corrêa¹,

Alcântara²

2012

Rev. bras. farmacogn.

125

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Abstract:The Acanthaceae family is an important source of therapeutic drugs, and the ethnopharmacological knowledge of this family requires urgent documentation as several of its species are near extinction. Justicia is the largest genus of Acanthaceae, with approximately 600 species. The present work provides a review addressing the chemistry and pharmacology of the genus Justicia. In addition, the biological activities of compounds isolated from the genus are also covered. The chemical and pharmacological information in the present work may inspire new biomedical applications for the species of Justicia, considering atom economy, the synthesis of environmentally benign products without producing toxic by-products, the use of renewable sources of raw materials, and the search for processes with maximal efficiency of energy.

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Potent Cytotoxic Lignans from Justicia procumbens and Their Effects on Nitric Oxide and Tumor Necrosis Factor-α Production in Mouse Macrophages

Cited by 91 publications

References 16 publications

Justicidin A Inhibits the Transport of Tumor Necrosis Factor-α to Cell Surface in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

Justicidin A Inhibits the Transport of Tumor Necrosis Factor-α to Cell Surface in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

Caspase‐8 acts as a key upstream executor of mitochondria during justicidin A‐induced apoptosis in human hepatoma cells

Chemical constituents and biological activities of species of Justicia: a review

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