Potent inhibition of lipopolysaccharide-inducible nitric oxide synthase expression by dibenzylbutyrolactone lignans through inhibition of I-κBα phosphorylation and of p65 nuclear translocation in macrophages

Cho, Min Kyung; Park, Jeong Weon; Jang, Young Pyo; Kim, Young Choong; Kim, Sang Geon

doi:10.1016/s1567-5769(01)00153-9

Cited by 49 publications

(41 citation statements)

References 28 publications

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“…Wang et al also demonstrated that REB displays a bioactive action for attenuating excessive NO generation at inflammatory site [7]. Further studies showed that various components, such as Caffeic acid, diarctigenin, arctigenin, Lappaol F, and trachelogeninin root and leaves of burdock are responsible for suppression NO synthesis [11,25,26]. HPLC analysis and single quadrupole mass spectrometry of root and leaves of burdock showed that amounts of some of these components in root extract is more than leaves extract [1,4,13,14].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Immunomodulatory Properties of Root and Leaves of Arctium Lappa (Burdock) in Vitro

Ahmadabad

Behnamfar

Firizi

et al. 2017

Zahedan J Res Med Sci

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Background: The roots and leaves of Arctium lappa (burdock) have been used for different therapeutic purposes, especially for diseases linked to chronic inflammation. Objectives: The present study was designed to evaluate and compare the immunomodulatory activities of root extract of burdock and leaves extract of burdock in vitro. Methods: In this experimental study, PHA-or LPS-stimulated splenocytes were treated with different concentrations of root or leaves extract of burdock and proliferation of splenocytes measured by MTT assay. The levels of IFN-γ and IL4 in the supernatants of PHAstimulated splenocytes determined using ELISA. We also studied the effects of root and leaves extract of burdock on Nitric Oxide production by LPS-stimulated macrophages using the Griess reagent. The data were analyzed by one-way ANOVA followed by Tukey's post-test using GraphPad Prism software, version 5.0. Results: Our findings showed that both root and leaves extract of burdock have suppressive effects on LPS-stimulated splenocytes proliferation, IL-4 secretion from PHA-stimulated splenocytes, and NO production from LPS-stimulated macrophage and stimulatory effects on PHA-stimulated splenocytes proliferation, and IFN-γ secretion from PHA-stimulated splenocytes. Although both root and leaves extract of burdock had similar immunomodulatory effects in vitro, stronger immunomodulatory effects seen in root extract of burdock. Conclusions: According to our results, we suggest that root of burdock is better option than leaves of burdock in modulation immune responses and inflammations.

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Section: Discussionmentioning

confidence: 99%

Comparison of the Immunomodulatory Properties of Root and Leaves of Arctium Lappa (Burdock) in Vitro

Ahmadabad

Behnamfar

Firizi

et al. 2017

Zahedan J Res Med Sci

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“…Arctigenin, a phenylpropanoid dibenzylbutyrolactone lignan, potently inhibited iNOS expression and NO production through suppression of NF-κB activation and inhibition of I-κBα phosphorylation and p65 nuclear translocation in LPS-activated macrophages (Cho et al, 2002). In addition, arctigenin strongly inhibited the expression of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and IL-6, in LPS-stimulated RAW264.7 cells, THP-1 human monocyte-macrophage and differentiated human macrophage U937 (Cho et al, 2002;Zhao et al, 2009).…”

Section: Anti-inflammatory Effectsmentioning

confidence: 99%

“…In addition, arctigenin strongly inhibited the expression of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and IL-6, in LPS-stimulated RAW264.7 cells, THP-1 human monocyte-macrophage and differentiated human macrophage U937 (Cho et al, 2002;Zhao et al, 2009). Further study showed that arctigenin-induced inhibition of TNF-α production might be mediated by arctigenin's potent inactivation of mitogen-activated protein (MAP) kinases including ERK1/2, p38 kinase and JNK through the inhibition of MAP kinase kinase (MKK) activity, leading to inactivation of activator protein-1 (AP-1) (Cho et al, 2004;Zhao et al, 2009).…”

Section: Anti-inflammatory Effectsmentioning

confidence: 99%

A review of the pharmacological effects of Arctium lappa (burdock)

et al. 2010

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Abstract. Arctium lappa, commonly known as burdock, is being promoted/recommended as a healthy and nutritive food in Chinese societies. Burdock has been used therapeutically in Europe, North America and Asia for hundreds of years. The roots, seeds and leaves of burdock have been investigated in view of its popular uses in Traditional Chinese Medicine (TCM). In this review, the reported therapeutic effects of the active compounds present in the different botanical parts of burdock are summarized. In the root, the active ingredients have been found to "detoxify" blood in TCM term and promote blood circulation to the skin surface, improving the skin quality/texture and curing skin diseases like eczema. Antioxidants and anti-diabetic compounds have also been found in the root. In the seeds, some active compounds possess anti-inflammatory effects, and potent inhibitory effects on the growth of tumors such as the pancreatic carcinoma. In the leaf extract, the active compounds isolated can inhibit the growth of micro-organisms in the oral cavity. The medicinal uses of burdock in treating chronic diseases like cancers, diabetes and AIDS have been reported. However, it is also essential to be aware of the side-effects of burdock including contact dermatitis and other allergic/inflammatory responses that might be evoked by burdock.

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“…1 factor-κ B (NF-κB) (16). Atn can also enhance the chemosensitivity of several cancer cells (Hepg2, HeLa, and K562), to cisplatin by inhibiting the STAT3 signaling pathway at high doses (17).…”

Section: Introductionmentioning

confidence: 99%

Arctigenin inhibits triple-negative breast cancers by targeting CIP2A to reactivate protein phosphatase 2A

et al. 2017

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Abstract.We have shown that a novel STAT3 inhibitor arctigenin (Atn) induces significant cytotoxicity in triple-negative breast cancer (TNBC) cells. This study further delineated molecular mechanisms where by Atn triggered cytotoxicity in TNBC cells. We found Atn can also inhibit metastasis in TNBC cells through cancerous inhibitor of protein phosphatase 2A (CIP2A) pathway. CIP2A is an endogenous inhibitor of protein phosphatase 2A (PP2A), which can increase the migration and invasion of various cancer cells. PP2A is a tumor suppressor, which is functionally defective in various cancers. Atn-induced metastasis inhibition was associated with reactivation of PP2A, downregulation of CIP2A and Akt phosphorylation. Silencing CIP2A enhanced Atn-induced metastasis inhibition and apoptosis in TNBCs. Furthermore, ectopic expression of CIP2A or inhibition of PP2A in TNBC cells abolished the effects of Atn. In conclusion, we found that enhancement of PP2A activity by inhibition of CIP2A, at least in part, promotes the anti-metastasis effect induced by Atn. Our findings disclose the novel therapeutic mechanism of this targeted agent, and suggest the therapeutic potential and feasibility of developing PP2A enhancers as a novel anticancer strategy.

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Potent inhibition of lipopolysaccharide-inducible nitric oxide synthase expression by dibenzylbutyrolactone lignans through inhibition of I-κBα phosphorylation and of p65 nuclear translocation in macrophages

Cited by 49 publications

References 28 publications

Comparison of the Immunomodulatory Properties of Root and Leaves of Arctium Lappa (Burdock) in Vitro

Comparison of the Immunomodulatory Properties of Root and Leaves of Arctium Lappa (Burdock) in Vitro

A review of the pharmacological effects of Arctium lappa (burdock)

Arctigenin inhibits triple-negative breast cancers by targeting CIP2A to reactivate protein phosphatase 2A

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