Potential allergenicity of <i>Medicago sativa</i> investigated by a combined <scp>IgE</scp>‐binding inhibition, proteomics and <i>in silico</i> approach

Yakhlef, Marwa; Giangrieco, Ivana; Ciardiello, Maria Antonietta; Fiume, Immacolata; Mari, Adriano; Souiki, Lynda; Pocsfalvi, Gabriella

doi:10.1002/jsfa.10730

Cited by 11 publications

(15 citation statements)

References 46 publications

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“…However, the analysis of Sola l 3 isoforms as separate components, or as sum of the two detected isoforms added together, do not show correlation with the Ni treatments. The lack of this kind of correlation was con rmed, at least for red tomato, following the analysis of the LTP content performed with immunological inhibition tests consisting in the competition of extract components with the allergenic molecules spotted on the FABER biochip 37 . Conversely, IgE-binding inhibition results obtained with the green tomato suggest a possible Ni effect producing a slight increase in LTP content.…”

Section: Discussionmentioning

confidence: 99%

“…The allergens contained in the samples treated with Ni 300 were investigated by immunological tests and the results were compared with those of controls made of untreated tomato. Two samples of Ni 300treated red tomato and green tomato were analysed with the SPHIAa method 36 on the FABER system 37 .…”

Section: Analysis Of Allergens Content In the Tomato Samples By Ige Inhibition Testsmentioning

confidence: 99%

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Tomato (Solanum Lycopersicum L.) Response to Nickel Stress: Bioavailability, Accumulation and Allergenicity

Roccotiello

Nicosia

Pierdonà

et al. 2021

Preprint

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Vegetables represent a major source of Ni exposure. Environmental contamination and cultural practices can increase Ni amount in tomato posing significant risk for human health. This work assesses the tomato (Solanum lycopersicum L.) response to Ni on the agronomic yield of fruits and the related production of allergens.Two cultivars were grown in pots amended with Ni 0, 30, 60, 120, and 300 mg kg-1, respectively. XRF and ICP-MS analyses highlighted the direct increase of fruit Ni content respect to soil Ni, maintaining a stable biomass. Leaf water content increased at Ni 300 mg kg-1. Total protein content and individual allergenic components were investigated using biochemical (RP-HPLC and N-terminal amino acid sequencing) and immunological (inhibition tests of IgE binding by SPHIAa assay on the FABER® testing system) methodologies. Ni affected the fruit tissue concentration of pathogenesis-related proteins and relevant allergens (LTP, profilin, Bet v 1-like protein and TLP). This study elucidates for the first time that tomato reacts to exogenous Ni uptaking metal while changing its allergenic profiles, with potential double increasing of exposure risks for consumers. This evidence highlighted the importance of adequate choice of low-Ni tomato cultivars and practices to avoid Ni uptake by potentially contaminated matrices.

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Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Allergens Content In the Tomato Samples By Ige Inhibition Testsmentioning

confidence: 99%

Tomato (Solanum Lycopersicum L.) Response to Nickel Stress: Bioavailability, Accumulation and Allergenicity

Roccotiello

Nicosia

Pierdonà

et al. 2021

Preprint

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“…Several mass-spectrometry-based methodologies and strategies can be used to identify proteins in food extracts [ 23 , 65 , 66 , 67 , 68 ]. The simultaneous identification and quantification of traces of allergenic proteins in complex mixtures such as processed foods can also be achieved [ 69 , 70 , 71 ].…”

Section: Classical Analytical Methods Generally Used For Allergen Det...mentioning

confidence: 99%

“…In this review, we have rather focused our attention on the use of a new method, the multiplex allergen microarray-based immunoassay, in the detection of food allergenic proteins. In particular, we have analyzed some studies using an inhibition assay on a multiplex allergen biochip, namely the single point highest inhibition achievable assay (SPHIAa) [ 8 , 23 ]. The use of this method to detect and identify allergens in foods has not yet been reviewed in the available literature, probably because it is a quite recent and little exploited procedure.…”

Section: Introductionmentioning

confidence: 99%

Detection of Allergenic Proteins in Foodstuffs: Advantages of the Innovative Multiplex Allergen Microarray-Based Immunoassay Compared to Conventional Methods

Tuppo

Giangrieco

Tamburrini

et al. 2022

Foods

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Several factors can affect the allergen content and profile of a specific food, including processing procedures often leading to a decrease in allergenicity, although no change, or even an increase, have also been reported. Evaluation of the effectiveness of a processing procedure requires the availability of reliable methodologies to assess the variation in molecules able to induce allergic reactions in the analyzed food. Conventional and innovative strategies and methodologies can be exploited to identify allergenic proteins in foodstuffs. However, depending on the specific purposes, different methods can be used. In this review, we have critically reviewed the advantages of an innovative method, the multiplex allergen microarray-based immunoassay, in the detection of allergens in foodstuffs. In particular, we have analyzed some studies reporting the exploitation of an IgE-binding inhibition assay on multiplex allergen biochips, which has not yet been reviewed in the available literature. Unlike the others, this methodology enables the identification of many allergenic proteins, some of which are still unknown, which are recognized by IgE from allergic patients, with a single test. The examined literature suggests that the inhibition test associated with the multiplex allergen immunoassay is a promising methodology exploitable for the detection of IgE-binding proteins in food samples.

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“…Signal intensities are then used to calculate the arbitrary units, FIU (FABER International Units), correlated with the level of IgE bound to each allergen spot. To obtain information on the content of possible allergens in the strawberry samples, the SPHIAa (Pasquariello et al, 2012;Tuppo et al, 2019) (Yakhlef et al, 2021) was performed on the FABER platform. Sera of allergic patients for which the IgE profile was already known was used as providers of IgE recognizing specific allergenic proteins spotted on the FABER biochip.…”

Section: Testing Systemmentioning

confidence: 99%

Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting

Stanly

Kim

Antonucci

et al. 2021

Front. Bioeng. Biotechnol.

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Background: Nanometer-sized membrane-surrounded vesicles from different parts of plants including fruits are gaining increasing attention due to their anti-inflammatory and anticancer effects demonstrated by in vitro and in vivo studies, and as nanovectors for molecular delivery of exogenous substances. These nanomaterials are very complex and contain a diverse arsenal of bioactive molecules, such as nucleic acids, proteins, and lipids. Our knowledge about the transport of allergens in vesicles isolated from plant food is limited today.Methods: Here, to investigate the allergenicity of strawberry-derived microvesicles (MVs), nanovesicles (NVs), and subpopulations of NV, we have set up a multidisciplinary approach. The strategy combines proteomics-based protein identification, immunological investigations, bioinformatics, and data mining to gain biological insights useful to evaluate the presence of potential allergens and the immunoglobulin E (IgE) inhibitory activity of vesicle preparations.Results: Immunological test showed that several proteins of strawberry-derived vesicles compete for IgE binding with allergens spotted on the FABER biochip. This includes the known strawberry allergens Fra a 1, Fra a 3, and Fra a 4, and also other IgE-binding proteins not yet described as allergens in this food, such as gibberellin-regulated proteins, 2S albumin, pectate lyase, and trypsin inhibitors. Proteomics identified homologous sequences of the three strawberry allergens and their isoforms in total protein extract (TPE) but only Fra a 1 and Fra a 4 in the vesicle samples. Label-free quantitative proteomic analysis revealed no significant enrichment of these proteins in strawberry vesicles with respect to TPE.Conclusion: Immunological tests and bioinformatics analysis of proteomics data sets revealed that MVs and NVs isolated from strawberries can carry functional allergens their isoforms as well as proteins potentially allergenic based on their structural features. This should be considered when these new nanomaterials are used for human nutraceutical or biomedical applications.

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Potential allergenicity of Medicago sativa investigated by a combined IgE‐binding inhibition, proteomics and in silico approach

Cited by 11 publications

References 46 publications

Tomato (Solanum Lycopersicum L.) Response to Nickel Stress: Bioavailability, Accumulation and Allergenicity

Tomato (Solanum Lycopersicum L.) Response to Nickel Stress: Bioavailability, Accumulation and Allergenicity

Detection of Allergenic Proteins in Foodstuffs: Advantages of the Innovative Multiplex Allergen Microarray-Based Immunoassay Compared to Conventional Methods

Crosstalk Between the Immune System and Plant-Derived Nanovesicles: A Study of Allergen Transporting

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