Potential and future perspectives of thraustochytrids in bioremediation

Contreras, Patricio Guzmán; Oviedo, Claudia

doi:10.1007/s13762-022-04740-5

Cited by 9 publications

(3 citation statements)

References 139 publications

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“…Thraustochytrids are fast-growing heterotrophic organisms that can use alternative and low-cost carbon sources for cultivation, for example, lignocellulosic biomass [ 47 ] and waste from pig industries [ 71 ]. This nutritional plasticity allows efficient use of resources and decreases the costs associated with production, and desired characteristics in a strain with commercial potential [ 72 , 73 , 74 , 75 ]. The increase in ARA productivity in the Lng2 U. visurgensis strain mediated by nutritional variation is a key factor to be considered for future studies by optimizing the culture medium.…”

Section: Discussionmentioning

confidence: 99%

Screening and Identification of Coastal Chilean Thraustochytrids for Arachidonic Acid Production: Biotechnological Potential of Ulkenia visurgensis Lng2-Strain

et al. 2023

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Thraustochytrids are unicellular heterotrophic marine protists that have been described as producing a high content of polyunsaturated fatty acids (PUFAs). Among them, arachidonic acid (ARA) stands out as a precursor of several mediators of pivotal importance for the immune system. However, the biotechnological potential of thraustochytrids for ARA production has not been developed. The objective of this study is to isolate and identify native strains from different Chilean coastal environments and evaluate in vitro the effect of culture parameters such as C/N ratio (19 and 33) and temperature (15 °C and 23 °C) on biomass production and arachidonic acid content. A total of nine strains were identified and classified into four genera of the Thraustochitridae family. The Lng2 strain with 99% identity belongs to the species Ulkenia visurgenis and was the most prominent one for ARA production. Temperature had an effect on the PUFA profile but not on the ARA content nor on the biomass yield. Additionally, the C/N ratio has been identified as a key parameter. The ARA productivity increased by 92% (from 0.6 to 8.3 ARA mg/g-DW) and its total biomass by 62.7% (from 1.9 to 5.1 g/L) at a high C/N ratio (33) as compared to the control.

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Section: Discussionmentioning

confidence: 99%

Screening and Identification of Coastal Chilean Thraustochytrids for Arachidonic Acid Production: Biotechnological Potential of Ulkenia visurgensis Lng2-Strain

et al. 2023

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“…However, most interesting of all is the fact that the biomass enriched with LC-PUFAs from THs could be used to be added as raw material at the beginning of a production process 8,9 or also be used as a byproduct in other processes that require enrichment with LC-PUFAs or other metabolites that THs produce (for example, astaxanthin). This is where the use of THs enriched biomass plays an important role in the CE, since it is well known that all processes generate waste, however, when using THs crops with various residual sources (liquid and solid as well), have the option of incorporating this microbial biomass into the processes, thus avoiding having to dispose of the waste or treat it at the cost that this entails.…”

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confidence: 99%