Potential and whole-genome sequence-based mechanism of elongated-prismatic magnetite magnetosome formation in Acidithiobacillus ferrooxidans BYM

Magnetosome yield characteristics modeling of acidithiobacillus ferrooxidans in airlift bioreactor using response surface methodology

“…34 Furthermore, the sequences with high identity to the genes related to magnetosome chain formation such as mamK and mamJ were not found in A. ferrooxidans . 35 Therefore, the findings revealed that the magnetosomes are dispersed very well in cell of A. ferrooxidans BYM were supported by the results from physiological characteristics, cell magnetism, and genomic analysis genome.…”

Phenomic and transcriptomic analyses reveal the sequential synthesis of Fe 3 O 4 nanoparticles in Acidithiobacillus ferrooxidans BYM

“…For intermittent sterilization, the sublimed sulfur needs to be heated to 100°C at atmospheric pressure, boiled for 30 min, and repeated three times after cooling. The 0K medium contained 1.2g/L (NH 4 ) 2 SO 4 , 0.1 g/L KCl, 0.5 g/L K 2 HPO 4 , 0.5 g/L MgSO 4 ·7H 2 O, 2.94 g/L C 6 H 12 O 7 , and 0.01 g/L Ca(NO 3 ) 2 ( 23 ). The 0K-FeSO 4 ·7H 2 O and 0K-S media were adjusted to pH 1.75 and 4.00 under sterile conditions, respectively.…”

“…The first part of A. ferrooxidans BYM cells was fixed overnight with glutaraldehyde (2.5%, vol/vol) at 4°C, fixed with 1% (wt/vol) osmium tetroxide for 1 h, dehydrated through a graded ethanol series (70%, 80%, 90%, and 100%), and embedded in Epon 812 resin. UC7 ultramicrotome (Co. Leica Microsystems GmbH, Germany) was used to obtain ultrathin sections stained with both uranyl acetate and lead citrate and observed by the HITACHI H-7650 TEM (HITACHI, Tokyo, Japan) at a 120-kV accelerating voltage ( 23 ). Based on the TEM images, the number and size of Fe 3 O 4 nanoparticles of all cells in the vision (at least 100 cells) were counted under different magnifications.…”

“…As one of the hot topics in microbiology, the mechanistic insights into the magnetosome synthesis are based mainly on the MTB and rarely on non-MTB. The A. ferrooxidans BYM genome (3.2 Mb) contains a circular chromosome with 58.54% GC content, 3,260 ORF numbers, three CRISPRs, and a plasmid of 56.44% GC content and 54 ORF numbers ( 23 ). Although previous study showed that the several genes of A. ferrooxidans shared relatively high similarity with magnetosome genes from MTB, they do not belong to MAI, and no region similar to MAI is found in the genome of A. ferrooxidans ( 23 ).…”

“…The A. ferrooxidans BYM genome (3.2 Mb) contains a circular chromosome with 58.54% GC content, 3,260 ORF numbers, three CRISPRs, and a plasmid of 56.44% GC content and 54 ORF numbers ( 23 ). Although previous study showed that the several genes of A. ferrooxidans shared relatively high similarity with magnetosome genes from MTB, they do not belong to MAI, and no region similar to MAI is found in the genome of A. ferrooxidans ( 23 ). A. ferrooxidans Fe 3 O 4 nanoparticles are irregularly dispersed in the cells, which is different from the chain arrangement of MTB ( 10 ).…”