Potential Anticancer Activity of Myricetin in Human T24 Bladder Cancer Cells Both In Vitro and In Vivo

Abstract

Myricetin, a naturally occurring phytochemical, has potent anticancer-promoting activity and contributes to the chemopreventive potential of several foods. In this preliminary study, we evaluate the chemopreventive potential of myricetin against bladder cancer and its mechanism of action. The results of a MTT assay showed that myricetin was able to inhibit the viability and proliferation of T24 cells in a dose- and time-dependent manner. It also promoted cell cycle arrest at G2/M in a dose-dependent manner and induced apoptosis detected by flow cytometry and DNA fragmentation analysis. Treatment with myricetin led to G2/M cell cycle arrest in T24 cells by downregulation of Cyclin B1 and cyclin-dependent kinase cdc2. Myricetin-induced apoptosis correlates with the modulation of Bcl-2 family proteins and activation of the caspase-3. Myricetin also inhibited the phosphorylation of Akt, whereas the phosphorylation of p38 MAPK was enhanced. Myricetin had a significantly reduced T24 cell migration that was accompanied by a decreasing MMP-9 expression in vitro. Furthermore, myricetin treatment significantly inhibited the tumor growth on T24 bladder cancer xenografts model. These findings suggest that myricetin has potential anticancer activity and could be an important chemoprevention agent for bladder cancer.

Myricetin inhibits advanced glycation end product (AGE)-induced migration of retinal pericytes through phosphorylation of ERK1/2, FAK-1, and paxillin in vitro and in vivo

“…Furthermore, myricetin inhibits matrix metalloproteinase-2 protein expression and enzymatic activity in colorectal carcinoma cells [45]. Although myricetin induces apoptosis in cancer cells, the precise impact and related molecular mechanism of myricetin on migration and invasion are still unclear [39]. As anticipated, myricetin strongly inhibited retinal pericyte migration in our study (Fig.…”

“…6A), a flavonoid commonly found in edible berries, significantly reduces cancer cell migration [22,39]. We investigated the ability of myricetin or PD98059 to modulate the phosphorylation of the MEK-1 downstream signaling proteins ERK1/2, FAK-1, and paxillin.…”

Collected Research on Phytonutrients: Flavonoids

“…The effect of myricetin in the suppression of tumor growth was also exhibited in T24 (refers to specific gene cells) bladder cancer cells (Sun et al, 2012) as well as pancreatic cancer cells (Phillips et al, 2011). It also induced cell death by apoptosis of the carcinogenic cells in both types of cancer, as well as blocking cell-signaling pathways and altering enzyme activities to prevent tumor growth.…”

Flavones and Flavonols may have Clinical Potential as CK2 Inhibitors in Cancer Therapy

“…Here are citations for the xenograft studies: apigenin, 65-81 luteolin, 111-122 kaempferol, 123 fisetin, [124][125][126] quercetin, 127-145 myrcetin. 146 There are at least 53 published studies in which flavones or flavonols have decreased the growth of human xenografts in nude mice.…”