Potential antioxidant effects of Narcissus tazetta phenolic compounds against cadmium chloride–induced hepatotoxicity in Swiss albino mice

Abstract

Narcissus tazetta (Amaryllidaceae) is a medicinal plant, widely used for cut owers and potted ornamental plant in Tunisia ora. The current study evaluated the phenolic composition and antioxidant properties of its ower extracts and investigated its potential protective activity against cadmium chloride (CdCl 2 )-induced hepatotoxicity in mice. Adult Swiss mice were exposed either to CdCl 2 (0.16 mg/kg bw) or to Narcissus tazetta extract (100 or mg/kg bw) or to their combination. Cadmium chloride and Narcissus tazetta ower extracts were given by intraperitoneal injection and gavage respectively for eight consecutive days. Results indicated that ethanolic ower extracts contained high levels of total phenolic and avonoid contents along with a strong total antioxidant and 2,2-Diphenyl-1-picrylhydrazyl free radical scavenging activities. High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) analysis identi ed eight phenolic compounds, including rutin, Kaempferol glycosides and chlorogenic acids. The extract also exhibited marked hepato-protective effects against CdCl 2 toxicity by reducing hepatic levels of malondialdehyde, advanced oxidation protein products, hydrogen peroxide, metallothioneins and DNA degradation. Additionally, co-administration of Narcissus tazetta ower extracts lowered the plasma activities of transaminases, gamma glutamyl transpeptidase and lactate dehydrogenase, and increased hepatic levels of reduced glutathione, non-protein thiols, vitamin C, and catalase activity. The hepatoprotective effects of the extract were demonstrated by histopathological improvement of liver disorders. The current study provided ethnopharmacological application of Narcissus tazetta ower extracts against CdCl 2 induced oxidative stress, suggesting its chemoprevention role of its phenolic compounds as a natural antioxidant.

Qualitative and Quantitative Estimation of Total Phenols in Narcissus tazettaL. Bulbs

“…In their study on Narcissus tazetta flowers, [28] found that flavonoids were more prevalent than phenolic components; for every 5 kg of the ethanolic extract, total phenolic was 64.14 mg/ml, while flavonoids were 70.79 mg/ml. In the Narcissus tazetta flower, [29] discovered substantial concentrations of total polyphenols and flavonoids.…”