Potential Application of Intestinal Organoids in Intestinal Diseases

Liu, Wenxiu; Wang, Qian; Bai, Yanrui; Xiao, Han; Li, Zhunduo; Wang, Yan; Wang, Qi; Yang, Jing; Sun, Hui

doi:10.1007/s12015-023-10651-w

Cited by 2 publications

(1 citation statement)

References 130 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cultivating intestinal organoids requires a small amount of tissue, and both endoscopic biopsy and surgical specimens can be used as tissue sources. Moreover, the mucosa of both inflammatory and non-inflammatory sites in the intestine can form intestinal organoids in vitro ( Liu et al, 2023 ). These results indicate that gut-like organs hold great promise as reliable instruments for studying IBD pathogenesis and drug screening.…”

Section: Application Of Organoids In Ibdmentioning

confidence: 99%

The application of organoids in colorectal diseases

Liu,

Wang,

Luan

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

Intestinal organoids are a three-dimensional cell culture model derived from colon or pluripotent stem cells. Intestinal organoids constructed in vitro strongly mimic the colon epithelium in cell composition, tissue architecture, and specific functions, replicating the colon epithelium in an in vitro culture environment. As an emerging biomedical technology, organoid technology has unique advantages over traditional two-dimensional culture in preserving parental gene expression and mutation, cell function, and biological characteristics. It has shown great potential in the research and treatment of colorectal diseases. Organoid technology has been widely applied in research on colorectal topics, including intestinal tumors, inflammatory bowel disease, infectious diarrhea, and intestinal injury regeneration. This review focuses on the application of organoid technology in colorectal diseases, including the basic principles and preparation methods of organoids, and explores the pathogenesis of and personalized treatment plans for various colorectal diseases to provide a valuable reference for organoid technology development and application.

show abstract

Section: Application Of Organoids In Ibdmentioning

confidence: 99%

The application of organoids in colorectal diseases

Liu,

Wang,

Luan

et al. 2024

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

A Human Biomimetic Intestinal Mucosa Model to Study Gastrointestinal Development and Disease

Dei,

Calitz,

Korsten

et al. 2024

Preprint

View full text Add to dashboard Cite

The intestinal mucosa plays a vital role in nutrient absorption, drug metabolism, and pathogen defence. Advances in single-cell technologies have highlighted the specialised roles of various cell types that execute these diverse functions. Aside from intestinal epithelial cells, fibroblasts play an essential role in regulating the extracellular matrix and controlling pro- inflammatory signalling, and antigen-presenting cells (macrophages and dendritic cells) maintain intestinal homeostasis and immune responses. The incorporation of such cellular complexity within the existingin vitromodels of the human intestine is currently challenging. To address this, we developed a human intestinal model that accurately mimics the mucosal cellular environment comprising intestinal epithelial cells, intestinal fibroblasts, and antigen presenting cells. This model includes co-cultures of adult and foetal cells, facilitating studies on barrier function, inflammation, and viral infections. It replicates extracellular matrix deposition, Paneth cell differentiation, immune interactions, and can be used to model host- pathogen interactions. Our advanced co-culture model improves the physiological relevance ofin vitrostudies, enabling the exploration of epithelial-mesenchymal-immune crosstalk and its role in intestinal health and disease.

show abstract

Potential Application of Intestinal Organoids in Intestinal Diseases

Cited by 2 publications

References 130 publications

The application of organoids in colorectal diseases

The application of organoids in colorectal diseases

A Human Biomimetic Intestinal Mucosa Model to Study Gastrointestinal Development and Disease

Contact Info

Product

Resources

About